Zoltán Villányi,István Gyurján,Viktor Stéger et al.
Zoltán Villányi et al.
The authors have developed a simple, cost-saving experimental design, plaque-based competitive hybridization (PBCH), for genome-wide identification of genes differentially expressed in different tissues. PBCH offers advantages in comparison...
Comparative Study
Journal of biomolecular screening. 2008 Jan;13(1):80-4. DOI:10.1177/1087057107310876 2008
Small-scale production of Burkholderia cepacia ATCC21808 lipase adapted to high-throughput screening [0.03%]
Burkholderia cepacia ATCC21808脂肪酶突变体的微量生产及其高通量筛选适应性研究
Sophie Puech-Guenot,Vincent Lafaquière,David Guieysse et al.
Sophie Puech-Guenot et al.
The screening of variant libraries of recombinant Burkholderia cepacia ATCC21808 lipase generated in Escherichia coli is limited by expression difficulties that are mainly due to the formation of inclusion bodies. To circumvent these diffic...
Development of a fission yeast-based high-throughput screen to identify chemical regulators of cAMP phosphodiesterases [0.03%]
基于裂殖酵母的高通量筛选体系用于识别环磷酸腺苷磷酸二酯酶化学调节因子的发展研究
F Douglas Ivey,Lili Wang,Didem Demirbas et al.
F Douglas Ivey et al.
Cyclic nucleotide phosphodiesterases (PDEs) comprise a superfamily of enzymes that serve as drug targets in many human diseases. There is a continuing need to identify high-specificity inhibitors that affect individual PDE families or even ...
Pharmacological profiling of chemokine receptor-directed compounds using high-content screening [0.03%]
基于高内涵筛选的化学趋化因子受体导向化合物的药理学研究
Dorothea Haasen,Susanne Merk,Peter Seither et al.
Dorothea Haasen et al.
High-content screening, typically defined as automated fluorescence microscopy combined with image analysis, is now well established as a means to study test compound effects in cellular disease-modeling systems. In this work, the authors e...
Cellular phenotype recognition for high-content RNA interference genome-wide screening [0.03%]
高内涵RNA干扰全基因组筛选中的细胞表型识别
Jun Wang,Xiaobo Zhou,Pamela L Bradley et al.
Jun Wang et al.
Genome-wide, cell-based screens using high-content screening (HCS) techniques and automated fluorescence microscopy generate thousands of high-content images that contain an enormous wealth of cell biological information. Such screens are k...
Comparison of miniaturized time-resolved fluorescence resonance energy transfer and enzyme-coupled luciferase high-throughput screening assays to discover inhibitors of Rho-kinase II (ROCK-II) [0.03%]
微型时间分辨荧光共振能量转移与酶耦合型萤火虫高通量筛选法发现Rho激酶Ⅱ(ROCKⅡ)抑制剂的比较研究
Thomas Schröter,Dmitriy Minond,Amiee Weiser et al.
Thomas Schröter et al.
Kinases are important drug discovery targets for a wide variety of therapeutic indications; consequently, the measurement of kinase activity remains a common high-throughput screening (HTS) application. Recently, enzyme-coupled luciferase-k...
Comparative Study
Journal of biomolecular screening. 2008 Jan;13(1):17-28. DOI:10.1177/1087057107310806 2008
Time-resolved fluorescence-based assay for the determination of alkaline phosphatase activity and application to the screening of its inhibitors [0.03%]
一种测定碱性磷酸酶活性及其抑制剂筛选的时域荧光分析法研究
Petra Schrenkhammer,Ina C Rosnizeck,Axel Duerkop et al.
Petra Schrenkhammer et al.
A single-step end point method is presented for determination of the activity of the enzyme alkaline phosphatase (ALP) using the effect of enhancement of fluorescence of the easily accessible europium(III)-tetracycline 3:1 complex (Eu(3)TC)...
Quantitative whole-cell cytochrome P450 measurement suitable for high-throughput application [0.03%]
适合高通量应用的定量全细胞细胞色素P450测定法
Wayne A Johnston,Weiliang Huang,James J De Voss et al.
Wayne A Johnston et al.
The recombinant expression of cytochrome P450 enzymes involved in drug metabolism is of interest to the pharmaceutical and biotechnological industries due to the versatile catalytic properties of these enzymes. Accurate quantification of cy...
Median absolute deviation to improve hit selection for genome-scale RNAi screens [0.03%]
基于基因组尺度的RNAi筛选改进命中选择的中位数绝对偏差法
Namjin Chung,Xiaohua Douglas Zhang,Anthony Kreamer et al.
Namjin Chung et al.
High-throughput screening (HTS) of large-scale RNA interference (RNAi) libraries has become an increasingly popular method of functional genomics in recent years. Cell-based assays used for RNAi screening often produce small dynamic ranges ...
Development of homogeneous 384-well high-throughput screening assays for Abeta1-40 and Abeta1-42 using AlphaScreen technology [0.03%]
一种用于α屏技术的阿贝塔1-40和阿贝塔1-42同质384孔高通量筛选模型的发展方法
Philip G Szekeres,Kaitlin Leong,Theresa A Day et al.
Philip G Szekeres et al.
Amyloid beta (Abeta) peptides are the major constituent of amyloid plaques, one of the hallmark pathologies of Alzheimer's disease. Accurate and precise quantitation of these peptides in biological fluids is a critical component of Alzheime...
Comparative Study
Journal of biomolecular screening. 2008 Feb;13(2):101-11. DOI:10.1177/1087057107312778 2008