Using the protein chip to screen agonists and antagonists of the androgen receptor [0.03%]
应用蛋白芯片筛选雄激素受体的激动剂和拮抗剂
Yong Zhou,Ailin Liu,Wei Wang et al.
Yong Zhou et al.
Based on its important physical and pathological function, the androgen receptor (AR) is regarded as a significant drug target. In this report, the authors describe a novel strategy of protein chip technology to screen agonists and antagoni...
Rapid assay of trace immunoglobulin M by a new immunonanogold resonance scattering spectral probe [0.03%]
一种新的免疫纳米金共振散射光谱探针快速测定微量免疫球蛋白M
Zhiliang Jiang,Lili Wei,Mingjing Zou et al.
Zhiliang Jiang et al.
Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to for...
Hoan Vu,Ngoc B Pham,Ronald J Quinn
Hoan Vu
The authors describe first a proof-of-concept experiment to show direct affinity screening using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) is a rapid and informative approach for natu...
Characterization of 5(6)-carboxy-2,'7'-dichlorofluorescein transport by MRP2 and utilization of this substrate as a fluorescent surrogate for LTC4 [0.03%]
5(6)-羧基-2',7'-二氯荧光素通过MRP2的转运以及将其底物作为LTC4的荧光替代物的应用特征化研究
Krisztina Heredi-Szabo,Emese Kis,Eva Molnar et al.
Krisztina Heredi-Szabo et al.
MRP2 (ABCC2) is an efflux transporter expressed on the apical membrane of polarized cells. This protein has a major role in the biliary elimination of toxic compounds from the liver. As MRP2 transports many endogenous compounds, including L...
High-throughput screening for daunorubicin-mediated drug resistance identifies mometasone furoate as a novel ABCB1-reversal agent [0.03%]
高效筛选出丹诺霉素介导的耐药药物并鉴定出莫米松糠酸酯为新型ABCB1逆转剂
Stuart S Winter,Debbie M Lovato,Hadya M Khawaja et al.
Stuart S Winter et al.
The overexpression of P-glycoprotein, encoded by the ATP Binding Cassette B1 (ABCB1) gene, contributes to multidrug resistance (MDR) and is considered one of the major obstacles to successful cancer chemotherapy. The authors previously deve...
A high-throughput hybridoma selection method using fluorometric microvolume assay technology [0.03%]
一种使用荧光微量检测技术的高通量杂交瘤筛选方法
Rozanne Lee,Mylinh Tran,Mark Nocerini et al.
Rozanne Lee et al.
Monoclonal antibodies (mAb) are not only useful reagents but also represent a promising type of therapeutics due to their high affinity and exquisite specificity for their antigens. A critical step in mAb generation is to identify antigen-s...
Label-free primary screening and affinity ranking of fragment libraries using parallel analysis of protein panels [0.03%]
基于蛋白质面板的平行分析的无标记片段库初筛及亲和力排序方法
Markku D Hämäläinen,Andrei Zhukov,Maria Ivarsson et al.
Markku D Hämäläinen et al.
The authors present fragment screening data obtained using a label-free parallel analysis approach where the binding of fragment library compounds to 4 different target proteins can be screened simultaneously using surface plasmon resonance...
Identification of inhibitors for MDM2 ubiquitin ligase activity from natural product extracts by a novel high-throughput electrochemiluminescent screen [0.03%]
一种从天然产物提取物中鉴定MDM2泛素连接酶抑制剂的新型高通量电化学发光筛选方法
Christy A Sasiela,David H Stewart,Jirouta Kitagaki et al.
Christy A Sasiela et al.
High-throughput screening technologies have revolutionized the manner in which potential therapeutics are identified. Although they are the source of lead compounds for ~65% of anticancer and antimicrobial drugs approved by the Food and Dru...
Use of FLIPR membrane potential dyes for validation of high-throughput screening with the FLIPR and microARCS technologies: identification of ion channel modulators acting on the GABA(A) receptor [0.03%]
采用FLIPR膜电位染料对离子通道调制剂进行高通量筛选的验证：作用于GABAA受体上的离子通道调制剂的鉴定
Christoph Joesch,Emelie Guevarra,Serge P Parel et al.
Christoph Joesch et al.
Fluorometric imaging plate reader (FLIPR) membrane potential dyes (FMP-Red-Dye and FMP-Blue-Dye) were evaluated for the detection of compounds acting either as positive allosteric modulators or agonists on the GABA(A) receptor (GABA(A)R). A...
Systematic and simultaneous gene profiling of 84 drug-metabolizing genes in primary human hepatocytes [0.03%]
人原代肝细胞中84种药物代谢基因的系统化及同步基因检测
Baitang Ning,Stacey Dial,Yanyang Sun et al.
Baitang Ning et al.
Drug-metabolizing enzymes are an important battery of proteins that are involved in drug metabolism, xenobiotic detoxification, and drug-induced toxicity. Systematic, efficient, and simultaneous evaluation of drug-metabolizing gene expressi...