A high-throughput screen for endothelial lipase using HDL as substrate [0.03%]
以HDL作为底物的内皮细胞脂酶高效筛选体系
Paul M Keller,Timothy Rust,Dennis J Murphy et al.
Paul M Keller et al.
Endothelial lipase (EL) is a 482-amino-acid protein from the triglyceride lipase gene family that uses a Ser-His-Asp triad for catalysis. Its expression in endothelial cells and preference for phospholipids rather than triglycerides are uni...
A direct immunoassay assessment of streptavidin- and N-hydroxysuccinimide-modified biochips in validation of serological TNFalpha responses in hemophagocytic lymphohistiocytosis [0.03%]
评估血噬性淋巴组织细胞增生症患者血清中肿瘤坏死因子α的直接免疫测定法及N-羟基琥珀酰亚胺和链霉亲和素修饰生物芯片的验证方法
Weidong Du,Xueling Ma,E Marion Schneider
Weidong Du
The authors report 2 biochip platforms on gold manufactured by either nanoscale biotinylated self-assembled architectures to streptavidin surface or proteins containing free NH(2) groups to N-hydroxysuccinimide (NHS)-activated surfaces and ...
Evaluating PI3 kinase isoforms using Transcreener ADP assays [0.03%]
用Transcreener ADP检测试剂盒评估PI3激酶同种型
Tony A Klink,Karen M Kleman-Leyer,Andrew Kopp et al.
Tony A Klink et al.
Development of drugs targeting lipid kinases has been delayed by the lack of robust screening assays. Methods are needed that can accommodate the presentation of different acceptor substrates in the optimal lipid environment. The Transcreen...
Multiplexed assays by high-content imaging for assessment of GPCR activity [0.03%]
基于高内涵成像的多联检测试剂盒用于G蛋白偶联受体活性检测
D A Ross,S Lee,V Reiser et al.
D A Ross et al.
G-protein-coupled receptors (GPCR) participate in many disease pathways and represent the largest family of therapeutic targets. Thus, great investments are made to discover drugs modulating GPCR-mediated events. Among functional assays for...
Marjo Simonen,Yvonne Ibig-Rehm,Gabriele Hofmann et al.
Marjo Simonen et al.
The mevalonate pathway leads to synthesis of cholesterol and isoprenoid lipids. Prenyltransferases attach the isoprenoid lipids to the C-terminus of several small guanosine triphosphate-binding proteins. The prenyl groups are essential for ...
Klaas J Wierenga,Kent Lai,Peter Buchwald et al.
Klaas J Wierenga et al.
Inherited deficiency of galactose-1-phosphate uridyltransferase (GALT) can result in a potentially lethal disorder called classic galactosemia. Although the neonatal lethality associated with this disease can be prevented through early diag...
Screening for antiviral inhibitors of the HIV integrase-LEDGF/p75 interaction using the AlphaScreen luminescent proximity assay [0.03%]
用于筛选HIV整合酶-LEDGF/p75相互作用抗病毒抑制剂的AlphaScreen邻近连接发光测定法
Yan Hou,Debra E McGuinness,Andrew J Prongay et al.
Yan Hou et al.
Small-molecule inhibitors of HIV integrase (HIV IN) have emerged as a promising new class of antivirals for the treatment of HIV/AIDS. The compounds currently approved or in clinical development specifically target HIV DNA integration and w...
Screening for antiviral inhibitors of the HIV integrase-LEDGF/p75 interaction using the AlphaScreen luminescent proximity assay [0.03%]
应用AlphaScreen发光接近均相技术筛选HIV整合酶-LEDGF/p75相互作用的抗病毒抑制剂
Yan Hou,Debra E McGuinness,Andrew J Prongay et al.
Yan Hou et al.
Small-molecule inhibitors of HIV integrase (HIV IN) have emerged as a promising new class of antivirals for the treatment of HIV/AIDS. The compounds currently approved or in clinical development specifically target HIV DNA integration and w...
Integrating experimental and analytic approaches to improve data quality in genome-wide RNAi screens [0.03%]
结合实验和分析方法提高基因组范围RNAi筛选的数据质量
Xiaohua Douglas Zhang,Amy S Espeseth,Eric N Johnson et al.
Xiaohua Douglas Zhang et al.
RNA interference (RNAi) not only plays an important role in drug discovery but can also be developed directly into drugs. RNAi high-throughput screening (HTS) biotechnology allows us to conduct genome-wide RNAi research. A central challenge...
Integrating experimental and analytic approaches to improve data quality in genome-wide RNAi screens [0.03%]
整合实验和分析方法以提高基因组范围RNAi筛选的数据质量
Xiaohua Douglas Zhang,Amy S Espeseth,Eric N Johnson et al.
Xiaohua Douglas Zhang et al.
RNA interference (RNAi) not only plays an important role in drug discovery but can also be developed directly into drugs. RNAi high-throughput screening (HTS) biotechnology allows us to conduct genome-wide RNAi research. A central challenge...