Lorenz M Mayr,Peter Fuerst
Lorenz M Mayr
High-throughput screening (HTS) is a well-established process in lead discovery for pharma and biotech companies and is now also being set up for basic and applied research in academia and some research hospitals. Since its first advent in ...
A homogeneous enzyme fragment complementation-based beta-arrestin translocation assay for high-throughput screening of G-protein-coupled receptors [0.03%]
基于同源酶片段互补的β- arrestin重定位检测方法在G蛋白偶联受体高通量筛选中的应用研究
Xiaoning Zhao,Adrie Jones,Keith R Olson et al.
Xiaoning Zhao et al.
G-protein-coupled receptors (GPCRs) represent one of the largest gene families in the human genome and have long been regarded as valuable targets for small-molecule drugs. The authors describe a new functional assay that directly monitors ...
Profiling protein tyrosine phosphorylation: a quantitative 45-plex peptide-based immunoassay [0.03%]
蛋白质酪氨酸磷酸化谱分析：一种定量的45-肽类免疫测定法
Timothy K Nadler,Christine Rauh-Adelmann,Cheryl Murphy et al.
Timothy K Nadler et al.
Cellular homeostasis and responses to stimuli are mediated by complex signaling network events dominated by changes in protein phosphorylation states. Understanding information flow in the network is essential for correlating signaling chan...
A novel method for determination of the affinity of protein: protein interactions in homogeneous assays [0.03%]
一种测定均相体系中蛋白质-蛋白质亲和力的新型方法研究
Philip Newton,Paula Harrison,Stephen Clulow
Philip Newton
Nonradioactive homogeneous assays are widely used to screen for inhibitors of biomolecular interactions. To ensure optimal sensitivity for the detection of competitive inhibitors, reagent concentrations should be fixed at or below the K(D) ...
A high-throughput screening assay for small molecules that disrupt yeast cell integrity [0.03%]
用于筛选可破坏酵母细胞完整性的化合物的高通量培养基筛查模型及阳性结果的确认
Damian J Krysan,Louis Didone
Damian J Krysan
Lead compounds for antifungal drug development are urgently needed because invasive fungal infections are an important cause of morbidity and mortality in immunocompromised patients. Here, a high-throughput screening assay for small molecul...
High-throughput screening-based identification of paramyxovirus inhibitors [0.03%]
基于高通量筛选的副粘病毒抑制剂的鉴定
Jeong-Joong Yoon,Dhruv Chawla,Tanja Paal et al.
Jeong-Joong Yoon et al.
Several members of the paramyxovirus family constitute major human pathogens that, collectively, are responsible for major morbidity and mortality worldwide. In an effort to develop novel therapeutics against measles virus (MV), a prominent...
HTRF-based assay for microsomal prostaglandin E2 synthase-1 activity [0.03%]
基于HTRF的微粒体前列腺素E2合成酶-1活性检测体系
Eric R Goedken,Andrew I Gagnon,Gary T Overmeyer et al.
Eric R Goedken et al.
Microsomal prostaglandin E2 synthase-1 (mPGES-1) catalyzes the formation of prostaglandin E2 (PGE2) from the endoperoxide prostaglandin H2 (PGH2). Expression of this enzyme is induced during the inflammatory response, and mouse knockout exp...
A TR3/Nur77 peptide-based high-throughput fluorescence polarization screen for small molecule Bcl-B inhibitors [0.03%]
基于TR3/Nur77肽的高通量荧光偏振筛选的小分子Bcl-B抑制剂筛查研究
Kenneth W Yip,Paulo H C Godoi,Dayong Zhai et al.
Kenneth W Yip et al.
Nuclear receptor TR3/Nur77/NR4A1 binds several antiapoptotic Bcl-2-family proteins (Bcl-B, Bcl-2, Bfl-1) in a non-BH3-dependent manner. A 9-amino-acid peptide derived from full-length TR3 with polyarginine tail (TR3-r8) recapitulates TR3's ...
Automated patch-clamp technique: increased throughput in functional characterization and in pharmacological screening of small-conductance Ca2+ release-activated Ca2+ channels [0.03%]
全自动膜片钳技术在小导电容钙离子释放激活钙离子通道的功能表征和药理筛选中的通量增加
Rikke L Schrøder,Søren Friis,Morten Sunesen et al.
Rikke L Schrøder et al.
The suitability of an automated patch clamp for the characterization and pharmacological screening of calcium release-activated calcium (CRAC) channels endogenously expressed in RBL-2H3 cells was explored with the QPatch system. CRAC curren...
Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates [0.03%]
用于多孔板中固体支持物反向转染的siRNA测定多重化的实验流程
Holger Erfle,Beate Neumann,Phill Rogers et al.
Holger Erfle et al.
Solid-phase reverse transfection on cell microarrays is a high-throughput method for the parallel transfection of mammalian cells. However, the cells transfected in this way have been restricted so far to microscopy-based analyses. Analysis...