A high-throughput liposome substrate assay with automated lipid extraction process for PI 3-kinase [0.03%]
一种用于PI 3-激酶的高通量脂质体底物测定法及其自动化过程中的脂质提取方法
Trupti Lingaraj,John Donovan,Zhi Li et al.
Trupti Lingaraj et al.
The signaling pathways involving lipid kinase class I phosphatidylinositol 3-kinases (PI 3-kinases) regulate cell growth, proliferation, and survival. Class I PI 3-kinases catalyze the conversion of PI (4,5)P(2) to PI (3,4,5)P(3), which act...
Genome-wide oligonucleotide microarray analysis of gene-expression profiles of Taiwanese patients with anaplastic astrocytoma and glioblastoma multiforme [0.03%]
台湾国家胶质细胞瘤及星形细胞瘤患者基因表现分析
Chia-Lin Chang
Chia-Lin Chang
This study investigated the relationship between gene expression and disease based on the expression profiles of tissue-specific genes, with the aim of discovering the candidate genes associated with disease risk as diagnostic markers. The ...
Identification of small molecule inhibitors of beta-amyloid cytotoxicity through a cell-based high-throughput screening platform [0.03%]
通过基于细胞的大规模筛选平台鉴定能够抑制β淀粉样蛋白毒性的小分子抑制剂
K I Seyb,E R Schuman,J Ni et al.
K I Seyb et al.
Calpain activation is hypothesized to be an early occurrence in the sequence of events resulting in neurodegeneration, as well as in the signaling pathways linking extracellular accumulation of beta-amyloid (Abeta) peptides and intracellula...
Grace K Y Chan,Bart A Lutterbach,Bo-Sheng Pan et al.
Grace K Y Chan et al.
Historically, only relatively low-throughput or expensive methods have been available to measure cell migration. Hepatocyte growth factor (HGF) is a ligand for the tyrosine kinase receptor Met that, in addition to mediating proliferation an...
Compound profiling using a panel of steroid hormone receptor cell-based assays [0.03%]
基于类固醇激素受体的细胞系实验评估化合物谱型
Jennifer M Wilkinson,Steven Hayes,David Thompson et al.
Jennifer M Wilkinson et al.
A major focus in the current discovery of drugs targeting nuclear receptors (NRs) is identifying drugs with reduced side effects by improving selectivity, not only from other receptors but also by selective modulation of the NR of interest....
An analysis of normalization methods for Drosophila RNAi genomic screens and development of a robust validation scheme [0.03%]
果蝇RNA干扰基因组筛选的归一化方法分析及验证体系的构建
Amy M Wiles,Dashnamoorthy Ravi,Selvaraj Bhavani et al.
Amy M Wiles et al.
Genome-wide RNA interference (RNAi) screening allows investigation of the role of individual genes in a process of choice. Most RNAi screens identify a large number of genes with a continuous gradient in the assessed phenotype. Screeners mu...
Analysis of interactions of signaling proteins with phage-displayed ligands by fluorescence correlation spectroscopy [0.03%]
运用荧光相关光谱分析信号蛋白与噬菌体展示配体相互作用
Helmi R M Schlaman,Kristiane Schmidt,Dorien Ottenhof et al.
Helmi R M Schlaman et al.
Fluorescent correlation spectroscopy (FCS) was used to measure binding affinities of ligands to ligates that are expressed by phage-display technology. Using this method we have quantified the binding of the 14-3-3 signaling protein to arti...
New antitrichomonal drug-like chemicals selected by bond (edge)-based TOMOCOMD-CARDD descriptors [0.03%]
基于bond（边缘）的TOMOCOMD-CARDD描述符选择的新抗滴虫药类化合物
Alfredo Meneses-Marcel,Oscar M Rivera-Borroto,Yovani Marrero-Ponce et al.
Alfredo Meneses-Marcel et al.
Bond-based quadratic indices, new TOMOCOMD-CARDD molecular descriptors, and linear discriminant analysis (LDA) were used to discover novel lead trichomonacidals. The obtained LDA-based quantitative structure-activity relationships (QSAR) mo...
Antiretroviral therapy-induced functional modification of IgG4 and IgM responses in HIV-1-infected individuals screened by an allosteric biosensor [0.03%]
allosteric生物传感器筛选出的HIV-1感染者抗逆转录病毒治疗引起的IgG4和IgM反应的功能改变
Rosa María Ferraz,Miguel Angel Martínez,Rafael Cubarsi et al.
Rosa María Ferraz et al.
We have explored the effect of antiretroviral drugs on the antiviral immune response in human immunodeficiency virus-1 (HIV-1)-infected patients by using an enzymatic immunosensor that detects epitope-modifying anti-gp41 antibodies. By this...
Novel dual-reporter preclinical screen for antiastrocytoma agents identifies cytostatic and cytotoxic compounds [0.03%]
针对抗胶质瘤药物的新颖双报告预临床筛选鉴定细胞毒性及非毒性化合物
Jessica J Hawes,John D Nerva,Karlyne M Reilly
Jessica J Hawes
Astrocytoma/glioblastoma is the most common malignant form of brain cancer and is often unresponsive to current pharmacological therapies and surgical interventions. Despite several potential therapeutic agents against astrocytoma and gliob...