Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch [0.03%]
体外进化核糖开关和适配子特异高效结合四环素的结构基础研究
Hong Xiao,Thomas E Edwards,Adrian R Ferré-D&#x;Amaré
Hong Xiao
The tetracycline aptamer is an in vitro selected RNA that binds to the antibiotic with the highest known affinity of an artificial RNA for a small molecule (Kd approximately 0.8 nM). It is one of few aptamers known to be capable of modulati...
Oksana M Subach,Illia S Gundorov,Masami Yoshimura et al.
Oksana M Subach et al.
We used a red chromophore formation pathway, in which the anionic red chromophore is formed from the neutral blue intermediate, to suggest a rational design strategy to develop blue fluorescent proteins with a tyrosine-based chromophore. Th...
Cross-strand split tetra-Cys motifs as structure sensors in a beta-sheet protein [0.03%]
跨链的四半胱氨酸基元可作为β折叠蛋白质中的结构传感器
Beena Krishnan,Lila M Gierasch
Beena Krishnan
We have designed "split tetra-Cys motifs" that bind the biarsenical fluorescein dye 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein (FlAsH) across strands of a model beta-rich protein. Our strategy was to divide the linear FlAsH binding tetr...
A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding [0.03%]
一种革兰氏阳性菌的σ因子全长度结构及其不能结合DNA的独特构象
Edmund C Schwartz,Alexander Shekhtman,Kaushik Dutta et al.
Edmund C Schwartz et al.
The sigma factors are the key regulators of bacterial transcription initiation. Through direct read-out of promoter DNA sequence, they recruit the core RNA polymerase to sites of initiation, thereby dictating the RNA polymerase promoter-spe...
Distinct structural elements dictate the specificity of the type III pentaketide synthase from Neurospora crassa [0.03%]
结构元件决定了嗜青霉菌型III五聚茋合酶的特异性
Sheryl B Rubin-Pitel,Houjin Zhang,Trang Vu et al.
Sheryl B Rubin-Pitel et al.
The fungal type III polyketide synthase 2'-oxoalkylresorcylic acid synthase (ORAS) primes with a range of acyl-Coenzyme A thioesters (C4-C20) and extends using malonyl-Coenzyme A to produce pyrones, resorcinols, and resorcylic acids. To gai...
Covalent and noncovalent intermediates of an NAD utilizing enzyme, human CD38 [0.03%]
利用NAD的酶人CD38共价和非共价中间体
Qun Liu,Irina A Kriksunov,Hong Jiang et al.
Qun Liu et al.
Enzymatic utilization of nicotinamide adenine dinucleotide (NAD) has increasingly been shown to have fundamental roles in gene regulation, signal transduction, and protein modification. Many of the processes require the cleavage of the nico...
Characterization of the alnumycin gene cluster reveals unusual gene products for pyran ring formation and dioxan biosynthesis [0.03%]
alnumycin基因簇的特征揭示了用于吡喃环形成和二氧杂环生物合成的非常规基因产物
Terhi Oja,Kaisa Palmu,Hanna Lehmussola et al.
Terhi Oja et al.
Alnumycin is closely related to the benzoisochromanequinone (BIQ) polyketides such as actinorhodin. Exceptional structural features include differences in aglycone tailoring that result in the unique alnumycin chromophore and the existence ...
Structure-activity relationship studies of the two-component lantibiotic haloduracin [0.03%]
卤杆菌素的结构活性关系研究
Lisa E Cooper,Amanda L McClerren,Anita Chary et al.
Lisa E Cooper et al.
The lantibiotic haloduracin consists of two posttranslationally processed peptides, Halalpha and Halbeta, which act in synergy to provide bactericidal activity. An in vitro haloduracin production system was used to examine the biological im...
Superagonistic fluorinated vitamin D3 analogs stabilize helix 12 of the vitamin D receptor [0.03%]
超强活性的氟化维生素D3类似物可稳定维生素D受体的第12螺旋
Guy Eelen,Noelia Valle,Yoshiteru Sato et al.
Guy Eelen et al.
Side chain fluorination is often used to make analogs of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] resistant to degradation by 24-hydroxylase. The fluorinated nonsteroidal analogs CD578, WU515, and WY1113 have an increased prodifferentiating a...
Platinum drug adduct formation in the nucleosome core alters nucleosome mobility but not positioning [0.03%]
铂类药物加合物的核小体核心改变核小体运动但不影响定位
Bin Wu,Curt A Davey
Bin Wu
Nucleosome positioning and reorganization regulate DNA site exposure in chromatin. Platinum anticancer agents form DNA adducts that disrupt nuclear activities, triggering apoptosis. Mechanistic insight would aid in the development of improv...