Pitfalls in the detection of t(11;22) translocation by fluorescence in situ hybridization and RT-PCR: a single-blinded study [0.03%]
荧光原位杂交和RT-PCR检测t(11;22)易位的陷阱：一项单盲研究
Nicolaus Friedrichs,Lydia Kriegl,Christopher Poremba et al.
Nicolaus Friedrichs et al.
The t(11;22) translocation is a diagnostic hallmark of various small round-cell tumors. This study correlates the performance of fluorescence in situ hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR) in the d...
Methylation of p16INK4a is a non-rare event in cervical intraepithelial neoplasia [0.03%]
p16INK4a甲基化在宫颈上皮内瘤变中较为常见
Suki Kang,Jungsik Kim,Hong Bae Kim et al.
Suki Kang et al.
The cell cycle inhibitor, p16INK4a may be a useful surrogate biomarker of cervical intraepithelial neoplasia (CIN); however, there is currently no consensus of p16INK4a genetic alterations throughout the multiple step process of CIN. Our go...
JC virus chromogenic in situ hybridization in brain biopsies from patients with and without PML [0.03%]
进行和未进行PML脑活检的JC病毒染色原位杂交检测结果
Gary W Procop,Rose C Beck,James D Pettay et al.
Gary W Procop et al.
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the JC polyoma virus. Electron microscopy and immunohistochemistry are the traditional methods of confirming the presence of...
Assessment of microsatellite instability in very small microdissected samples and in tumor samples that are contaminated with normal DNA [0.03%]
评估微卫星不稳定性的方法在取样极少的标本以及肿瘤组织中正常DNA含量较高的情况下依然可靠
Cynthia L Trusky,Antonia R Sepulveda,Jennifer L Hunt
Cynthia L Trusky
Microsatellite instability (MSI) testing is important for the management of young patients with colonic adenocarcinoma. Biopsies can be small and can be contaminated by normal cells. It is not known how sample size or contamination by non-n...
Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts [0.03%]
二维ΔΔCt计算法作为BCR-ABL P210转录本定量PCR数据分析的替代方法的验证研究
Alaina Arocho,Beiyun Chen,Marc Ladanyi et al.
Alaina Arocho et al.
Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder that is characterized by the presence of a reciprocal translocation between chromosomes 9 and 22 and results in the formation of the Philadelphia (Ph1) chromosome an...
Quantitative analysis of herpesvirus load in the lymph nodes of patients with histiocytic necrotizing lymphadenitis using a real-time PCR assay [0.03%]
采用实时定量PCR检测方法分析肉芽肿性淋巴结炎患者淋巴结内疱疹病毒载量
Nagako Maeda,Yoriko Yamashita,Hiroshi Kimura et al.
Nagako Maeda et al.
The cause of histiocytic necrotizing lymphadenitis (HNL) has been ascribed to viral infection, but its pathogenesis still remains unknown. Real-time PCR assays are useful not only for their sensitivity of detection but also for the quantita...
Improved DOP-PCR-based representational whole-genome amplification using quantitative real-time PCR [0.03%]
基于定量实时PCR的改进型DOP-PCR全基因组代表扩增法
Liliána Z Fehér,Margit Balázs,János Z Kelemen et al.
Liliána Z Fehér et al.
In many cases, only a minute amount of partially degraded genomic DNA can be extracted from archived clinical samples. Diverse whole-genome amplification methods are applied to provide sufficient amount of DNA for comparative genome hybridi...
Laser capture microdissection of epithelial cancers guided by antibodies against fibroblast activation protein and endosialin [0.03%]
靶向成纤维细胞活化蛋白和内糖萼素的激光捕获微切割上皮癌
Christian Rupp,Helmut Dolznig,Christina Puri et al.
Christian Rupp et al.
Transcriptional profiling of cancer biopsies is used extensively to identify expression signatures for specific cancer types, diagnostic and prognostic subgroups, and novel molecular targets for therapy. To broaden these applications, sever...
Mucinous colorectal cancers with chromosomal instability: a biologically distinct and aggressive subtype [0.03%]
染色体不稳定型黏液性结直肠癌：一种具有侵袭性的生物学亚型
Yoshihiro Kazama,Toshiaki Watanabe,Takamitsu Kanazawa et al.
Yoshihiro Kazama et al.
Colorectal cancers can progress through 2 pathways of genomic instability: microsatellite instability (MSI) and chromosomal instability (CSI). We investigated the influence of CSI and MSI on clinicopathological features and survival of 35 p...
CDH1 gene promoter hypermethylation in gastric cancer: relationship to Goseki grading, microsatellite instability status, and EBV invasion [0.03%]
胃癌中CDH1基因启动子高甲基化与Gotsek级别、微卫星不稳定状态及EB病毒侵袭的关系
Monika Zazula,Ana Maria Ferreira,Jacek P Czopek et al.
Monika Zazula et al.
Hypermethylation of the CDH1 promoter region seems to be the most common epigenetic mechanism in this gene silencing in gastric cancer. In this study, CDH1 promoter hypermethylation was observed in 54.8% (46/84) of the analyzed sporadic gas...