Real-time quantitative RT-PCR shows variable, assay-dependent sensitivity to formalin fixation: implications for direct comparison of transcript levels in paraffin-embedded tissues [0.03%]
实时定量RT-PCR显示福尔马林固定的影响不同且与所采用的测定方法有关：对石蜡包埋组织中转录本丰度直接比较的意义
Ina Koch,Julia Slotta-Huspenina,Regina Hollweck et al.
Ina Koch et al.
Real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) is a versatile tool for precise quantification of gene expression. Formalin-fixed and paraffin-embedded (FFPE) tissue is well suited for qRT-PCR, if RNA extrac...
Cervical tissue collection methods for RNA preservation: comparison of snap-frozen, ethanol-fixed, and RNAlater-fixation [0.03%]
宫颈取样方法对RNA保存的影响：立即将样本冷冻、乙醇固定和RNAlater固定的比较研究
Sophia S Wang,Mark E Sherman,Janet S Rader et al.
Sophia S Wang et al.
Promising molecular techniques may allow for testing of novel and complex hypotheses such as defining gene expression profiles in specific cells, tumors, or their microenvironments. For most large cancer epidemiologic and population-based s...
BRAF mutation analysis in fine needle aspiration (FNA) cytology of the thyroid [0.03%]
甲状腺细针穿刺细胞学中BRAF基因突变分析
Long Jin,Thomas J Sebo,Nobuki Nakamura et al.
Long Jin et al.
BRAF mutations have been detected in 30% to 80% of papillary thyroid carcinomas (PTC). Several detection methods for BRAF mutation have been reported, but a direct comparison between different assay methods has not been previously reported....
HER-2 gene amplification in Paget disease of the nipple and extramammary site: a chromogenic in situ hybridization study [0.03%]
切点Paget病HER2基因扩增的染色原位杂交检测研究
Michelle K Bianco,Mohammad A Vasef
Michelle K Bianco
Patients with human epidermal growth factor receptor 2 (HER-2) overexpressing breast carcinomas have a more aggressive clinical behavior and their tumors are often hormone receptor negative. However, the recently introduced anti-HER-2 antib...
Her-2/neu in barrett esophagus: a comparative study between histology, immunohistochemistry, and fluorescence in situ hybridization [0.03%]
巴雷特食管中HER-2/NEU的表达：组织化学、免疫组化和原位杂交比较研究
Elisa Rossi,Vincenzo Villanacci,Gabrio Bassotti et al.
Elisa Rossi et al.
Her-2/neu is a protooncogene frequently overexpressed in breast cancer, recently found to be also overexpressed in carcinoma arising on Barrett esophagus (BE). Immunohistochemistry and fluorescence in situ hybridization (FISH) are conventio...
A novel fixative improves opportunities of nucleic acids and proteomic analysis in human archive's tissues [0.03%]
一种新型固定剂改善了人类档案组织中核酸和蛋白质分析的机会
Giorgio Stanta,Stefano Pozzi Mucelli,Francesca Petrera et al.
Giorgio Stanta et al.
All tissues from biopsy or surgery origin are fixed and paraffin embedded as a routine procedure in the hospital departments of pathology. The traditional method of tissue preservation is the fixation in formalin, followed by paraffin embed...
Hui-yong Jiang,San-quan Zhang,Tong Zhao
Hui-yong Jiang
We invented a new method to make microarrays using nuclei extracted from paraffin-embedded tissues or cultured cells. A blank recipient paraffin block with 10 x 10 cores was constructed and sectioned to make the mold for the cell arrays. Th...
A simple and sensitive method for detecting major mutations within the tyrosine kinase domain of the epidermal growth factor receptor gene in non-small-cell lung carcinoma [0.03%]
一种检测非小细胞肺癌患者表皮生长因子受体酪氨酸激酶区主要突变的简便而灵敏的方法
Hiroaki Ohnishi,Kouki Ohtsuka,Akiko Ooide et al.
Hiroaki Ohnishi et al.
The detection of mutations in the epidermal growth factor receptor (EGFR) gene impacts therapeutic decision-making for non-small-cell lung carcinoma (NSCLC). Although direct sequencing has been most frequently used to detect EGFR mutations,...
Sarcoglycanopathies: a multiplex molecular analysis for the most common mutations [0.03%]
串联分析检测糖苷聚糖病常见突变
Telma L F Gouveia,Julia F O Paim,Rita C Pavanello et al.
Telma L F Gouveia et al.
Sarcoglycanopathies (SGpathies) are highly frequent among severely affected limb-girdle muscular dystrophy patients. On the basis of the findings of 5 common mutations in the 4 sarcoglycan (SG) genes in the Brazilian population, we standard...
Molecular techniques for identifying HCC origin and biology after orthotopic liver transplantation [0.03%]
分子技术在肝移植后识别肝细胞癌的起源及生物学特性中的应用
Valeria R Mas,Daniel G Maluf,Catherine I Dumur et al.
Valeria R Mas et al.
Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. Liver transplantation represents the potentially curative treatment for small HCC. Recurrence after surgical resection and liver transplantation remains one of the...