Rapid alpha-1-antitrypsin M-variant genotyping by primer-induced restriction analysis [0.03%]
通过引物诱导的限制性分析快速进行α-1抗胰蛋白酶M变异型基因分型
Mohsen Mohammadian Yajloo,Abbas Sahebghadam Lotfi,Seyed Alireza Mesbah Namin et al.
Mohsen Mohammadian Yajloo et al.
The 4 normal alleles of M1, M2, M3, and M4 are the most common gene products of the human alpha-1-antitrypsin (hAAT). Two single substitutions in M1 are responsible for M3 and M4, whereas 2 substitutions in M1 produce M2. Polymerase chain r...
Validation of rapid polymerase chain reaction-based detection of all length polymorphisms in the UGT 1A1 gene promoter [0.03%]
UGT1A1基因启动子区域所有长度多态性的快速聚合酶链反应检测的验证
Chih-Kang Huang,Alina Dulau,Chiao-Ju Su-Rick et al.
Chih-Kang Huang et al.
UDP glucuronosyltransferase (UGT) 1A1 gene promoter polymorphism can affect the expression level of the UGT 1A1 enzyme. The polymorphism consists of an insertion of a TA nucleotide sequence into a (TA)6TAA sequence in the gene promoter resu...
Correcting false gene expression measurements from degraded RNA using RTQ-PCR [0.03%]
利用实时定量RT-PCR校正降解RNA的假基因表达测量值
Matthias Port,Hans Ulrich Schmelz,Tanja Stassen et al.
Matthias Port et al.
This paper describes a method allowing correcting false gene expression measured on highly degraded RNA using real-time quantitative reverse transcription-polymerase chain reaction (RTQ-PCR). RNA was isolated from different models (in vitro...
Enhanced detection of TP53 mutations using a GC-clamp in denaturing high performance liquid chromatography [0.03%]
利用变性高效液相色谱中的GC夹板提高TP53基因突变的检测率
Timothy C Greiner
Timothy C Greiner
Although denaturing gradient gel electrophoresis (DGGE) is a highly effective technique for screening for TP53 mutations, the use of denaturing high performance liquid chromatography (DHPLC) is a growing methodology. This report describes a...
Epstein-Barr virus-associated primary lymphoepitheliomalike carcinoma of the esophagus [0.03%]
与Epstein-Barr病毒有关的原发性食管淋巴上皮瘤样癌
José Rafael Valbuena,Yasmina Retamal,Carolina Bernal et al.
José Rafael Valbuena et al.
Primary lymphoepitheliomalike carcinomas (LELC) of the esophagus are uncommon, with only 29 previously reported cases in the literature. Primary LELC of the esophagus is associated with Epstein-Barr virus (EBV). We herein report a 52-year-o...
Detection of opportunistic infections by low-density microarrays: a diagnostic approach for granulomatous lymphadenitis [0.03%]
低密度微阵列检测机会性感染：肉芽肿性淋巴结炎的诊断方法
Margarete Odenthal,Sina Koenig,Patrick Farbrother et al.
Margarete Odenthal et al.
In molecular diagnosis of infectious diseases often more than 1 pathogen has to be considered. As a consequence, a series of labor-intensive and time-consuming polymerase chain reaction (PCR) approaches specific for different putative patho...
Molecular detection of SS18-SSX fusion gene transcripts by cRNA in situ hybridization in synovial sarcoma using formalin-fixed, paraffin-embedded tumor tissue specimens [0.03%]
应用cRNA原位杂交技术检测福尔马林固定石蜡包埋滑膜肉瘤标本中SS18-SSX融合基因转录物
Shuichi Kanemitsu,Masanori Hisaoka,Shohei Shimajiri et al.
Shuichi Kanemitsu et al.
SS18-SSX fusion genes resulting from a chromosomal translocation t(X;18)(p11.2;q11.2) are a genetic hallmark of synovial sarcoma. Although such cytogenetic or molecular aberrations have mostly been detected by fluorescence in situ hybridiza...
Disturbed expression of the apoptosis regulators XIAP, XAF1, and Smac/DIABLO in gastric adenocarcinomas [0.03%]
胃腺癌中凋亡调节因子XIAP、XAF1和Smac/DIABLO的表达异常
Tomotaka Shibata,Csaba Mahotka,Nils Wethkamp et al.
Tomotaka Shibata et al.
Dysregulation of apoptosis plays an important role in carcinogenesis and tumor progression. Whereas x-linked inhibitor of apoptosis (XIAP) is a potent inhibitor of apoptosis, its antagonists second mitochondria-derived activator of caspases...
Tissue pretreatment with formic acid might lower HercepTest scores in breast cancer [0.03%]
甲酸预处理可能降低乳腺癌中HercepTest评分
Florian R Fritzsche,Glen Kristiansen,Andreas Boesl et al.
Florian R Fritzsche et al.
Creutzfeldt-Jakob disease and other prion diseases are diseases with yet not well-defined routes of transmission and infection. The safe processing of potentially contaminated tissue material remains a challenge for histologic laboratories....
Optimization of recovery of RNA from formalin-fixed, paraffin-embedded tissue [0.03%]
优化福尔马林固定石蜡包埋组织中RNA的提取方法
Joon-Yong Chung,Till Braunschweig,Stephen M Hewitt
Joon-Yong Chung
Formalin-fixed, paraffin-embedded (FFPE) tissue is the most common specimen available for application of diagnostic assays on tissue after microscopic examination. Not only is there a substantial archive of tissue available, but FFPE tissue...