Microsatellite analysis in serum DNA as a diagnostic tool for distinction of patients with unknown pancreatic masses [0.03%]
血清DNA微卫星分析作为鉴别未知胰腺肿物患者的诊断工具
Robin Wachowiak,Jussuf Kaifi,Heidi Schwarzenbach et al.
Robin Wachowiak et al.
The clinical distinction between cancer and chronic pancreatitis is difficult in patients with pancreatic masses. To test whether detection of aberrant serum DNA could assist in this important differential diagnosis, we tested a panel of 12...
Use of the real-time PCR assay in conjunction with MagNA Pure for the detection of mycobacterial DNA from fixed specimens [0.03%]
磁化珠提取法联合实时定量PCR技术检测固定标本中的结核杆菌DNA
Safedin H Beqaj,Randy Flesher,Gina R Walker et al.
Safedin H Beqaj et al.
Tuberculosis in immunocompromised patients is often caused by Mycobacterial species other than Mycobacterium tuberculosis. Thus, detection of and differentiation between M. tuberculosis and nontuberculosis species is necessary for diagnosis...
Gene expression profiling of Epstein-Barr virus-positive and -negative monomorphic B-cell posttransplant lymphoproliferative disorders [0.03%]
移植后单克隆B细胞淋巴增生性疾病的基因表达谱分析（EB病毒阳性及阴性疾病）
Fiona E Craig,Lawrence R Johnson,Stephen A K Harvey et al.
Fiona E Craig et al.
Although most posttransplant lymphoproliferative disorders (PTLD) are related to Epstein-Barr virus (EBV) infection, approximately 20% lack detectable EBV (EBV-). It is uncertain whether the latter cases are truly distinct from EBV+ PTLD or...
Dirk Schaudien,Wolfgang Baumgärtner,Christiane Herden
Dirk Schaudien
Standard curves are important tools in real-time quantitative polymerase chain reaction (PCR) to precisely analyze gene expression patterns under physiologic and pathologic conditions. Handling of DNA standards often implies multiple cycles...
A ribonuclease protection assay-based approach for analysis of angiogenic gene expression in archival tissues [0.03%]
基于存档组织中血管生成基因表达分析的核糖核酸酶保护测定方法
Antonella Naldini,Camilla Ardinghi,Daniela Massi et al.
Antonella Naldini et al.
Archival, formalin-fixed and paraffin-embedded tissues routinely stored in pathology departments represent an invaluable resource for retrospective molecular biology studies for diagnostic and prognostic purposes. In such specimens extracti...
Multiple molecular analyses from minimal cell quantities by sequential isolation and preamplification of DNA and RNA [0.03%]
通过依次分离和扩增DNA和RNA对微量细胞进行多重分子分析
Astrid Koehler,Leoni A Kunz-Schughart,Ferdinand Hofstaedter et al.
Astrid Koehler et al.
Molecular analyses of single or very few cells are often handicapped by low amounts of DNA or RNA from starting material. Specimens like biopsies, embryonic stem cells, or laser-microdissected tissues often do not provide nucleic acid quant...
Measurement of signaling pathway activities in solid tumor fine-needle biopsies by slide-based cytometry [0.03%]
基于载片的细胞计数法测量实体肿瘤细针活检标本中信号通路活性
Jörg Schwock,James C Ho,Ed Luther et al.
Jörg Schwock et al.
The application of molecular targeted therapies is expected to cause a modulation of cellular signaling pathway(s) that can be monitored by sequential biopsies. Fine-needle sampling (FNS) is an atraumatic and safe technique that can be repe...
Resolution of equivocal results with the Hybrid Capture II high-risk HPV DNA test: a cytologic/histologic review of 191 cases [0.03%]
混合捕捉二期高危型HPV DNA检测灰区结果的确证及回顾分析
Stewart M Knoepp,Diane L Kuebler,David C Wilbur
Stewart M Knoepp
Introduction: The Hybrid Capture II (HC II, Digene) high-risk human papilloma virus (HPV) (hrHPV) DNA test is an in vitro nucleic acid hybridization assay that uses enhanced chemiluminescence for the qualitative and semiq...
Profound inhibition of the PCR step of CF V3 multiplex PCR/OLA assay by the use of UV-irradiated plastic reaction tubes [0.03%]
紫外照射塑料反应管对CF V3多联PCR/OLA测定中PCR步骤的强抑制作用
David H Fox,Chih-Kang Huang,Juan Du et al.
David H Fox et al.
Supplies, such as bags of plastic reaction tubes, are sometimes left in the laminar flow hoods unintentionally while the ultraviolet (UV) lamp is illuminated overnight. In addition, UV irradiation is used for sterilization and amplicon inac...
False positive results of mitochondrial DNA depletion/deletion due to single nucleotide substitutions causing appearance of additional PvuII restriction sites [0.03%]
单核苷酸变异引起PvuII限制性内切酶位点增加假阳性结果导致线粒体DNA拷贝数减少/缺失检测结果增多
Anna Weglewska-Jurkiewicz,Joanna Jakóbkiewicz-Banecka,Ewa Pronicka et al.
Anna Weglewska-Jurkiewicz et al.
Human mitochondrial diseases are usually caused by dysfunction of mitochondrial DNA (mtDNA), particularly by point mutations, deletions, or depletions. In commonly used procedures for molecular diagnostics of mitochondrial dysfunction, one ...