Improved soluble expression of the gene encoding amylolytic enzyme Amo45 by fusion with the mobile-loop-region of co-chaperonin GroES in Escherichia coli [0.03%]
通过与分子伴侣GroES的可动环区域融合提高枯草芽孢杆菌淀粉酶Amo45在大肠杆菌中的可溶性表达
Lei Wang,Hildegard Watzlawick,Olafur Fridjonsson et al.
Lei Wang et al.
The gene encoding the amylolytic enzyme Amo45, originating from a metagenomic project, was retrieved by a consensus primer-based approach for glycoside hydrolase (GH) family 57 enzymes. Family 57 contains mainly uncharacterized proteins sim...
Use of bacteria for rapid, pH-neutral, hydrolysis of the model hydrophobic carboxylic acid ester p-nitrophenyl picolinate [0.03%]
利用细菌快速温和地水解模式疏水羧酸酯picoline-p-NPATES
Alexandra E Forest,Gordon G Goldstine,Yann Schrodi et al.
Alexandra E Forest et al.
Caulobacter crescentus, Escherichia coli and Bacillus subtilis cultures promote the hydrolysis of the model ester p-nitrophenyl picolinate (PNPP) at neutral pH with high efficiency. Hydrolysis is related to cell concentration, while the int...
Zhenbo Zhang,Aditi Banerjee,Krishna Baksi et al.
Zhenbo Zhang et al.
Mannosylphospho dolichol synthase (DPMS) plays a critical role in Glc(3)Man(9)GlcNAc(2)-PP-Dol (lipid-linked oligosaccharide, LLO) biosynthesis, an essential intermediate in asparagine-linked (N-linked) protein glycosylation. We have observ...
Isolation of autochthonous non-white rot fungi with potential for enzymatic upgrading of Venezuelan extra-heavy crude oil [0.03%]
分离土著非白腐真菌并通过其酶来升级委内瑞拉超重原油
Leopoldo Naranjo,Hector Urbina,Angela De Sisto et al.
Leopoldo Naranjo et al.
The increasing world demand for fuels makes it necessary to exploit the largest reserve of extra-heavy crude oil (EHCO) of the Orinoco Oil Belt from Venezuela. We propose the use of extracellular oxidative enzymes, in particular, lignin-deg...