Fluorescence-based approaches for monitoring membrane receptor oligomerization [0.03%]
基于荧光的方法监测膜受体寡聚化
Andrew Ha Clayton
Andrew Ha Clayton
Membrane protein structures are highly under-represented relative to water-soluble protein structures in the protein databank. This is especially the case because membrane proteins represent more than 30% of proteins encoded in the human ge...
Studying backbone torsional dynamics of intrinsically disordered proteins using fluorescence depolarization kinetics [0.03%]
利用荧光偏振动力学研究固有无序蛋白主链内部旋转动态性质
Debapriya DAS,Samrat Mukhopadhyay
Debapriya DAS
Intrinsically disordered proteins (IDPs) do not autonomously adopt a stable unique 3D structure and exist as an ensemble of rapidly interconverting structures. They are characterized by significant conformational plasticity and are associat...
Simli Dey,Sudipta Maiti
Simli Dey
Single-molecule photobleaching (smPB) technique is a powerful tool for characterizing molecular assemblies. It can provide a direct measure of the number of monomers constituting a given oligomeric particle and generate the oligomer size di...
Fluorescence microscopy applied to intracellular transport by microtubule motors [0.03%]
用于微管马达的细胞内运输的荧光显微镜技术
Divya Pathak,Shreyasi Thakur,Roop Mallik
Divya Pathak
Long-distance transport of many organelles inside eukaryotic cells is driven by the dynein and kinesin motors on microtubule filaments. More than 30 years since the discovery of these motors, unanswered questions include motor- organelle se...
A novel fluorescence microscopic approach to quantitatively analyse protein-induced membrane remodelling [0.03%]
一种新型荧光显微技术定量分析蛋白质诱导的膜重塑效应
Thomas J Pucadyil
Thomas J Pucadyil
Membrane remodelling or the bending and rupture of the lipid bilayer occurs during diverse cellular processes such as cell division, synaptic transmission, vesicular transport, organelle biogenesis and sporulation. These activities are brou...
Parijat Sarkar,Amitabha Chattopadhyay
Parijat Sarkar
Use of Green Fluorescent Protein (GFP) as a marker has revolutionized biological research in the last few decades. In this brief commentary, we reflect upon the success story of GFP and highlight a few lesser-known facets about GFP that add...
Kundan Sengupta
Kundan Sengupta
The genome of higher eukaryotes is non-randomly organized in the interphase nucleus. However, notwithstanding the absence of membrane bound sub-compartments, the nucleus coordinates a number of functions largely by organizing chromatin in a...
A parasporin from Bacillus thuringiensis native to Peninsular India induces apoptosis in cancer cells through intrinsic pathway [0.03%]
印度半岛土生芽孢杆菌苏云金芽胞菌变种的 parasporin 通过细胞凋亡诱导癌细胞的内在途径
Thomas Chubicka,Devaki Girija,Kizhakkeettil Deepa et al.
Thomas Chubicka et al.
Parasporins, a class of non-insecticidal crystal proteins of Bacillus thuringiensis (Bt) are being explored as promising anticancer agents due to their specific toxicity to cancer cells. The present study has identified 25 Bt isolates harbo...
Mahdi Rezaei,Mohammad Reza Naghavi,Abdolhadi Hosseinzadeh et al.
Mahdi Rezaei et al.
Here, a comprehensive endeavor is made to simultaneously scrutinize spatiotemporal oscillations of three imperative morphinan alkaloids (i.e. thebaine, codeine, and morphine) alongside dynamic transcriptional patterns of TYDC, SalAT, COR, T...
Antony J Durston,Joao Peres,Morrel H Cohen
Antony J Durston
During early embryonic development, the vertebrate main body axis is segmented from head-to-tail into somites. Somites emerge sequentially from the presomitic mesoderm (PSM) as a consequence of oscillatory waves of genetic activity, called ...