CRISPR-Cas12a coupled with universal gold nanoparticle strand-displacement probe for rapid and sensitive visual SARS-CoV-2 detection [0.03%]
用于快速灵敏可视化SARS-CoV-2检测的CRISPR-Cas12a联合通用型金纳米粒子链置换探针
Sitong Liu,Tie Xie,Xiaojing Pei et al.
Sitong Liu et al.
Point of care (POC) diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are particularly significant for preventing transmission of coronavirus disease 2019 (COVID-19) by any user at any given time and place. CRISPR/Ca...
Interference reduction isothermal nucleic acid amplification strategy for COVID-19 variant detection [0.03%]
用于新冠变异株检测的减少干扰等温核酸放大策略
Guodong Li,Chung-Nga Ko,Zikang Wang et al.
Guodong Li et al.
Common reference methods for COVID-19 variant diagnosis include viral sequencing and PCR-based methods. However, sequencing is tedious, expensive, and time-consuming, while PCR-based methods have high risk of insensitive detection in varian...
Peptide-based direct electrochemical detection of receptor binding domains of SARS-CoV-2 spike protein in pristine samples [0.03%]
基于肽的对原始样品中SARS-CoV-2刺突蛋白受体结合域的直接电化学检测
T H Vignesh Kumar,Sowmiya Srinivasan,Vinoth Krishnan et al.
T H Vignesh Kumar et al.
RNA isolation and amplification-free user-friendly detection of SARS-CoV-2 is the need of hour especially at resource limited settings. Herein, we devised the peptides of human angiotensin converting enzyme-2 (hACE-2) as bioreceptor at elec...
Dual ligand-induced photoelectrochemical sensing by integrating Pt/MoS2 heterostructure and Au polyhedra for sensitive detection of SARS-CoV-2 [0.03%]
通过集成Pt/MoS2异质结构和Au多面体实现双配体诱导的光电化学传感，灵敏检测SARS-CoV-2
Haolin Li,Jialin Zhao,Ting Wu et al.
Haolin Li et al.
The continuous evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with discovery of multiple mutants, has caused widespread panic and concern worldwide. The rapid antigen detection method via a single ligand recognit...
An ultrasensitive high-performance baicalin sensor based on C3N4-SWCNTs/reduced graphene oxide/cyclodextrin metal-organic framework nanocomposite [0.03%]
一种基于C3N4-SWCNTs/还原氧化石墨烯/环糊精金属有机骨架纳米复合材料的黄芩素传感器
Pengcheng Zhao,Linzi Huang,Hui Wang et al.
Pengcheng Zhao et al.
Baicalin (Bn) obtained from natural plants has been found to exhibit significant antiviral activity against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Herein, a novel ultrasensitive Bn electrochemical sensor was proposed ...
Digital phase detection approach and its application for AlN dual-mode differential surface acoustic wave sensing [0.03%]
基于数字相位检测的AlN双模差分表面声波传感方法研究
Guopeng Hu,Jianzeng Xu,Gregory W Auner et al.
Guopeng Hu et al.
Performance of a conventional phase detector implemented using analog circuitry is affected by, among other factors, the parameter value drifting of the analog components with environment (e.g., temperature) and time (i.e., aging). It is al...
Hijacking the self-replicating machine of bacteriophage for PCR-based cascade signal amplification in detecting SARS-CoV-2 viral marker protein in serum [0.03%]
劫持噬菌体自我复制机器进行聚合酶链式反应级联信号放大以检测血清中的SARS-CoV-2病毒标记蛋白
Jialei Du,Daili Xiang,Fushan Liu et al.
Jialei Du et al.
In this work, the nucleic acid detection of SARS-Cov-2 is extended to protein markers of the virus, utilizing bacteriophage. Specifically, the phage display technique enables the main protease of SARS-Cov-2 to control the self-replication o...
A photoelectrochemical immunosensor based on magnetic all-solid-state Z-scheme heterojunction for SARS-CoV-2 nucleocapsid protein detection [0.03%]
一种基于磁性全固态Z型异质结的光电解免疫传感器用于SARS-CoV-2核衣壳蛋白检测
Aijiao Guo,Fubin Pei,Shasha Feng et al.
Aijiao Guo et al.
Rapid, convenient and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is urgently needed to timely diagnosis of coronavirus pandemic (COVID-19) and control of the epidemic. In this study, a signal-off phot...
Systematically investigating the fluorescent signal readout of CRISPR-Cas12a for highly sensitive SARS-CoV-2 detection [0.03%]
系统调查CRISPR-Cas12a的荧光信号读取以实现高灵敏度检测SARS-CoV-2
Sitong Liu,Tie Xie,Zhaohe Huang et al.
Sitong Liu et al.
The CRISPR/Cas system is widely used for molecular diagnostics after the discovery of trans-cleavage activity, especially now with the COVID-19 outbreak. However, the majority of contemporary trans-cleavage activity-based CRISPR/Cas biosens...
Plasma-induced nanoparticle aggregation for stratifying COVID-19 patients according to disease severity [0.03%]
等离子体诱导的纳米粒子聚集根据疾病严重程度对COVID-19患者进行分类
Giulia Santopolo,Antonio Clemente,Marta González-Freire et al.
Giulia Santopolo et al.
Stratifying patients according to disease severity has been a major hurdle during the COVID-19 pandemic. This usually requires evaluating the levels of several biomarkers, which may be cumbersome when rapid decisions are required. In this m...