Nanopore with Transverse Nanoelectrodes for Electrical Characterization and Sequencing of DNA [0.03%]
横向纳米电极纳米孔用于DNA的电气表征和测序
Brian C Gierhart,David G Howitt,Shiahn J Chen et al.
Brian C Gierhart et al.
A DNA sequencing device which integrates transverse conducting electrodes for the measurement of electrode currents during DNA translocation through a nanopore has been nanofabricated and characterized. A focused electron beam (FEB) milling...
Microfluidic Protein Patterning on Silicon Nitride Using Solvent Extracted Poly(dimethylsiloxane) Channels [0.03%]
采用溶剂萃取型聚二甲基硅氧烷通道在氮化硅上进行微流控蛋白质图形化技术
Xinya He,David S Dandy,Charles S Henry
Xinya He
Biomolecular patterning is essential for the creation of sensing motifs that rely on receptor-ligand binding for selectivity. Microfluidic devices have the potential to aid in the development of simple, robust methods for biomolecular patte...
A System for Multiplexed Direct Electrical Detection of DNA Synthesis [0.03%]
一种用于直接电学检测DNA合成的多路复用系统
Erik P Anderson,Jonathan S Daniels,Heng Yu et al.
Erik P Anderson et al.
An electronic system for the multiplexed detection of DNA polymerization is designed and characterized. DNA polymerization is detected by the measurement of small transient currents arising from ion diffusion during polymerization. A transi...
Zeta-potential Analyses using Micro Electrical Field Flow Fractionation with Fluorescent Nanoparticles [0.03%]
采用荧光纳米颗粒进行微尺度电场脉冲色谱ζ电位分析
Moon-Hwan Chang,Dosi Dosev,Ian M Kennedy
Moon-Hwan Chang
Increasingly growing application of nanoparticles in biotechnology requires fast and accessible tools for their manipulation and for characterization of their colloidal properties. In this work we determine the zeta-potentials for polystyre...
Studies of metal ion binding by apo-metallothioneins attached onto preformed self-assembled monolayers using a highly sensitive surface plasmon resonance spectrometer [0.03%]
利用高灵敏度表面等离子体共振光谱仪研究预形成自组装单分子层结合清金属硫蛋白的金属离子bindings
Yintang Zhang,Maotian Xu,Yanju Wang et al.
Yintang Zhang et al.
The use of a flow-injection surface plasmon resonance (FI-SPR) spectrometer equipped with a bicell detector or a position-sensitive device for determining coordination of heavy metal ions (Cd(2+) and Hg(2+)) by surface-confined apo-metallot...
Resonance wavelength-dependent signal of absorptive particles in surface plasmon resonance-based detection [0.03%]
基于表面等离子体共振的吸收性粒子检测中的共振波长依赖信号变化规律研究
Elain Fu,Stephen A Ramsey,Jingyi Chen et al.
Elain Fu et al.
We describe the resonance wavelength-dependent signal of absorptive particles in surface plasmon resonance (SPR)-based detection using both modeling and experimental results. The particles, gold nanocages, have a significant absorption cros...
Antimicrobial peptides as new recognition molecules for screening challenging species [0.03%]
用于筛选疑难杂症的新型识别分子-抗菌肽
Nadezhda V Kulagina,Kara M Shaffer,Frances S Ligler et al.
Nadezhda V Kulagina et al.
The goal of this study was to evaluate binding of four targets of biodefense interest to immobilized antimicrobial peptides (AMPs) in biosensor assays. Polymyxins B and E, melittin, cecropins A, B, and P, parasin, bactenecin and magainin-1,...
Using Support Vector Machine Regression to Model the Retention of Peptides in Immobilized Metal-affinity Chromatography [0.03%]
用支持向量机回归预测金属 immobilization亲和色谱保留时间
B G Kermani,I Kozlov,P Melnyk et al.
B G Kermani et al.
Retention of histidine-containing peptides in immobilized metal-affinity chromatography (IMAC) has been studied using several hundred model peptides. Retention in a Nickel column is primarily driven by the number of histidine residues; howe...
Improving Blood Compatibility of Intravascular Oxygen Sensors Via Catalytic Decomposition of S-Nitrosothiols to Generate Nitric Oxide In Situ [0.03%]
通过催化分解S-硝基硫醇原位生成一氧化氮以提高血管内氧传感器的血液相容性
Yiduo Wu,Alvaro P Rojas,Grant W Griffith et al.
Yiduo Wu et al.
Reliable, real-time, in vivo sensing (intravascular) of blood gases and electrolytes remains a difficult challenge owing to biocompatibility issues that occur when chemical sensors are implanted into the blood stream. Recently, local releas...
Phosphate binding protein as the biorecognition element in a biosensor for phosphate [0.03%]
用于磷酸盐检测的生物传感器中的磷酸盐结合蛋白作为生物识别元件
Lyndon L E Salins,Sapna K Deo,Sylvia Daunert
Lyndon L E Salins
This work explores the potential use of a member of the periplasmic family of binding proteins, the phosphate binding protein (PBP), as the biorecognition element in a sensing scheme for the detection of inorganic phosphate (Pi). The select...