Frequency-domain lifetime measurements and sensing in highly scattering media [0.03%]
强散射介质中的频域寿命测量及传感技术研究
Henryk Szmackinski,Joseph R Lakowicz
Henryk Szmackinski
For clinical chemistry, or for non-invasive sensing through skin, it is often necessary to obtain quantitative information in highly scattering media. We describe two simple methods for fluorescence lifetime measurements in highly scatterin...
A bistable, multiport valve enables microformulators creating microclinical analyzers that reveal aberrant glutamate metabolism in astrocytes derived from a tuberous sclerosis patient [0.03%]
一种双稳定多端口阀能够实现微配方器的构建，该微临床分析仪可揭示取自结节性硬化症患者的星形胶质细胞中异常的谷氨酸代谢状况
Dusty R Miller,David K Schaffer,M Diana Neely et al.
Dusty R Miller et al.
There is a need for valves and pumps that operate at the microscale with precision and accuracy, are versatile in their application, and are easily fabricated. To that end, we developed a new rotary planar multiport valve to faithfully sele...
Development of a rapid and sensitive quantum dot nanobead-based double-antigen sandwich lateral flow immunoassay and its clinical performance for the detection of SARS-CoV-2 total antibodies [0.03%]
新型冠状病毒(SARS-CoV-2)总抗体检测的量子点纳米微球双抗原夹心试纸条免疫层析法的建立及其临床应用性能评估
Yaofeng Zhou,Yuan Chen,Wenjuan Liu et al.
Yaofeng Zhou et al.
Owing to the over-increasing demands in resisting and managing the coronavirus disease 2019 (COVID-19) pandemic, development of rapid, highly sensitive, accurate, and versatile tools for monitoring total antibody concentrations at the popul...
In Vitro Biosensing of β-Amyloid Peptide Aggregation Dynamics using a Biological Nanopore [0.03%]
基于生物纳米孔的体外检测β-淀粉样肽聚集动态的方法
Brian Lenhart,Xiaojun Wei,Brittany Watson et al.
Brian Lenhart et al.
Alzheimer's disease and other neurodegenerative disorders are becoming more prevalent as advances in technology and medicine increase living standards and life expectancy. Alzheimer's disease is thought to initiate development early in the ...
Alexander G Zestos,Michael D Nguyen,Brian L Poe et al.
Alexander G Zestos et al.
Carbon-fiber microelectrodes (CFMEs) are typically constructed from glass capillaries pulled to a fine taper or from a polyimide-coated capillary that is 90 μm in outer diameter. Here, a new fabrication method is developed to insulate carb...
Potassium and sodium measurements at clinical concentrations using phase-modulation fluorometry [0.03%]
临床浓度的钾和钠的相位调制荧光分析法测定
Henryk Szmacinski,Joseph R Lakowicz
Henryk Szmacinski
We describe the possibility of measuring sodium and potassium at the concentrations present in whole blood using lifetime-based sensing and phase-modulation fluorometry. The probe SBFO was shown to display changes in phase or modulation at ...
Henryk Szmacinski,Joseph R Lakowicz
Henryk Szmacinski
Time-resolved fluorescence spectroscopy is presently regarded as a research tool in biochemistry, biophysics and chemical physics. However, time-resolved methods can also be used for chemical sensing. Lifetime-based sensing has several adva...
Rapid point-of-care testing for SARS-CoV-2 virus nucleic acid detection by an isothermal and nonenzymatic Signal amplification system coupled with a lateral flow immunoassay strip [0.03%]
一种等温非酶信号放大系统结合侧向流免疫层析试纸条的快速现场检测SARS-CoV-2病毒核酸检测方法
Mingyuan Zou,Feiya Su,Rui Zhang et al.
Mingyuan Zou et al.
An outbreak of a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), began in December 2019. Accurate, rapid, convenient, and relatively inexpensive diagnostic methods for SARS-CoV-2 infection are important for pu...
Magnetic graphene quantum dots facilitate closed-tube one-step detection of SARS-CoV-2 with ultra-low field NMR relaxometry [0.03%]
磁性石墨烯量子点可促进SARS-CoV-2绝管一步检测的超低场NMR弛豫测量
Yongqiang Li,Peixiang Ma,Quan Tao et al.
Yongqiang Li et al.
The rapid and sensitive diagnosis of the highly contagious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the crucial issues at the outbreak of the ongoing global pandemic that has no valid cure. Here, we propose a S...
Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor [0.03%]
开发基于血管紧张素转换酶2的SARS-CoV-2衍生受体结合结构域生物传感器
Jung-Soo Suh,Heon-Su Kim,Tae-Jin Kim
Jung-Soo Suh
The global outbreak of coronavirus disease and rapid spread of the causative severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represent a significant threat to human health. A key mechanism of human SARS-CoV-2 infection is initi...