Resolution of space-group ambiguity and structure determination of nodamura virus to 3.3 A resolution from pseudo-R32 (monoclinic) crystals [0.03%]
由伪R32(单斜)晶型解析nodamura病毒空间群并将其结构精修至3.3埃分辨率
A Zlotnick,P Natarajan,S Munshi et al.
A Zlotnick et al.
Monoclinic crystals of nodamura virus (NOV) have two virus molecules per asymmetric unit. Packing analysis reveals a pseudo-rhombohedral (pseudo-C2 monoclinic) arrangement of particles in the actual P2(1) space group (a = 562.1, b = 354.1, ...
I Polikarpov,A Teplyakov,G Oliva
I Polikarpov
This paper describes an analysis of the optimum choice of the X-ray wavelength for macromolecular diffraction data collection. It is shown that there is no ultimate X-ray wavelength for protein crystallography and that the optimum wavelengt...
Comparative analysis of thaumatin crystals grown on earth and in microgravity [0.03%]
地面与微重力环境下thaumatin晶体生长的比较研究
J D Ng,B Lorber,R Giegé et al.
J D Ng et al.
The protein thaumatin was studied as a model macromolecule for crystallization in microgravity-environment experiments conducted on two US Space Shuttle missions (USML-2 and LMS). In this investigation, we have evaluated and compared the qu...
Crystals of the carotenoid protein from Arthrospira maxima containing uniformly oriented pigment molecules [0.03%]
含有均匀定向色素分子的极大螺旋藻类胡萝卜素蛋白晶体
C A Kerfeld,Y P Wu,C Chan et al.
C A Kerfeld et al.
Crystals of a carotenoid protein from the cyanobacterium Arthrospira maxima have been grown in space group C2 with unit-cell dimensions a = 219.6, b = 40.3, c = 75.5 A and beta = 95.5 degrees. The crystals diffract X-rays to 2.3 A resolutio...
Structure of monellin refined to 2.3 a resolution in the orthorhombic crystal form [0.03%]
-monellin的正交晶型结构分析至2.3┱分辨率
G Bujacz,M Miller,R Harrison et al.
G Bujacz et al.
The structure of orthorhombic crystals of monellin, a sweet protein extracted from African serendipity berries, has been solved by molecular replacement and refined to 2.3 A resolution. The final R factor was 0.150 for a model with excellen...
Lysozyme aggregation studied by light scattering. II. Variations of protein concentration [0.03%]
利用光散射研究溶菌酶聚集的第二种方式：蛋白质浓度的变化
Y Georgalis,P Umbach,J Raptis et al.
Y Georgalis et al.
Static and dynamic light scattering have been employed to investigate the behaviour of nucleating lysozyme solutions in the range between 0.34 and 3.08 mM. Preselected concentrations of NaC1 and (NH(4))(2)SO(4) have been used to screen the ...
Lysozyme aggregation studied by light scattering. I. Influence of concentration and nature of electrolytes [0.03%]
利用光散射研究溶菌酶的聚集。I.浓度和电解质种类的影响
Y Georgalis,P Umbach,J Raptis et al.
Y Georgalis et al.
Static and dynamic light scattering have been employed to investigate the behaviour of lysozyme solutions when varying the concentration of (NH(4))(2)SO(4) and NaCl for screening the repulsive forces between the monomers. At the initial agg...
Experiences from the structure determination of human cytomegalovirus protease [0.03%]
人巨细胞病毒蛋白酶结构测定的体会
L Tong,C Qian,W Davidson et al.
L Tong et al.
Several obstacles were encountered and overcome during the structure determination of human cytomegalovirus protease. Dehydration of crystals, by exposing them to higher concentrations of the precipitant, reduced the mosaicity of the crysta...
New techniques for applying anomalous-scattering and isomorphous-replacement data incorporated in ANOMIR - a general application package [0.03%]
应用于ANOMIR的异常散射和同晶置换数据诠释的新技术——一个通用软件包
M M Woolfson,Y Jia-Xing,F Hai-Fu
M M Woolfson
A computer package ANOMIR is described which can derive phases from anomalous scattering and/or isomorphous-replacement data in any combination. For anomalous scattering it incorporates five methods of applying one-wavelength data and three...
Polyalanine reconstruction from Calpha positions using the program CALPHA can aid initial phasing of data by molecular replacement procedures [0.03%]
用Calpha程序重构聚丙氨酸可有助于通过分子置换法初步相位判断实验的数据
R M Esnouf
R M Esnouf
The Calpha positions for a protein can provide a scaffold for the reconstruction of more complete models. Reconstructions can be by manual rebuilding, from geometric solutions to the constraints on main-chain torsion angles or from database...