Crystallization of chicken egg-white lysozyme from ammonium sulfate [0.03%]
硫酸铵为沉淀剂的溶菌酶结晶试验研究鸡蛋白色素蛋白质Lysozyme的结晶实验
E L Forsythe,E H Snell,M L Pusey
E L Forsythe
Chicken egg-white lysozyme was crystallized from ammonium sulfate over the pH range 4.0-7.8, with protein concentrations from 100 to 150 mg ml(-1). Crystals were obtained by vapor-diffusion or batch-crystallization methods. The protein crys...
A new iterative procedure for combining direct methods with solvent flattening - dealing with the phase ambiguity in protein crystallography [0.03%]
一种新的迭代过程：结合直接法和溶剂平坦化处理蛋白质晶体学中的相位歧义问题
Y Gu,C Zheng,Y Zhao et al.
Y Gu et al.
A new procedure for combining direct methods with the solvent-flattening technique is proposed for phasing single isomorphous replacement (SIR) or one-wavelength anomalous scattering (OAS) data of proteins. The new procedure differs from th...
A weighted rotation function [0.03%]
加权旋转函数
D I Svergun
D I Svergun
A simple probability expression is derived to discriminate the intermolecular vectors in the cross rotation function. The radial weighting function introduced takes into account both particle anisometry and crystal packing and can be incorp...
Crystallization and preliminary crystallographic studies on the mannose-specific lectin from garlic [0.03%]
大蒜专一性识别半乳糖的 Lectin 的结晶及初步晶体学研究
N R Chandra,T K Dam,A Surolia et al.
N R Chandra et al.
A mannose-specific agglutinin from garlic (Allium sativum) which forms part of a well conserved super-family of bulb lectins has been purified and crystallized by the hanging-drop vapour-diffusion technique, by equilibrating with a 20% solu...
Crystallization and preliminary X-ray diffraction studies of the Saccharomyces cerevisiae phospholipid-transfer protein Sec14p [0.03%]
酿酒酵母磷脂转移蛋白Sec14p的结晶及初步X射线衍射研究
B Sha,S E Phillips,V A Bankaitis et al.
B Sha et al.
The Saccharomyces cerevisiae phosphatidylinositol-transfer protein Secl4p catalyzes the exchange of phosphatidylinositol or phosphatidylcholine between membrane bilayers in vitro, and is an essential protein required for the budding of secr...
Crystallization and preliminary X-ray analysis of the Saccharomycopsis fibuligera glucoamylase expressed from the GLU1 gene in Escherichia coli [0.03%]
来自大肠杆菌的GLU1基因表达的Saccharomycopsis fibuligera淀粉酶的结晶及初步X射线分析
A Solovicová,J Gasperík,J Sevcík et al.
A Solovicová et al.
The active non-glycosylated glucoamylase, overexpressed from the Saccharomycopsis fibuligera GLU1 gene in Escherichia coli BL21(DE3), has been purified from the solubilized inclusion bodies and then renatured in vitro. Crystals of the recom...
Crystallization of an antitumour antibody SM3 complexed with a peptide epitope [0.03%]
抗肿瘤抗体SM3-表位肽复合物的结晶研究
P Dokurno,J M Lally,P A Bates et al.
P Dokurno et al.
SM3 antibody binds to a tumour-associated epitope on polymorphic epithelial mucin (PEM). Crystals of the Fab fragment of SM3 in complex with a peptide antigen were obtained by vapour diffusion against mother liquor containing acetate buffer...
C E McVey,S P Tolley,S H Done et al.
C E McVey et al.
A new crystal form of penicillin acylase (penicillin amidohydrolase, E.C. 3.5.1.11) from Escherichia coli W (ATCC 11105) is reported. The crystals were grown using a combination of hanging-drop and streak-seeding methods. The crystals are i...
The effects of metal binding on a nucleobase: the experimental charge density and electrostatic potential in 1H(+)-adeniniumtrichlorozinc(II) at 123 K and its relationship to that in adenine hydrochloride hemihydrate [0.03%]
论金属与碱基相互作用的影响：在123K下N-甲腺嘌呤三氯化锌(I)的实验电荷密度及其静电势与盐酸腺嘌呤半水合物的比较
L M Cunane,M R Taylor
L M Cunane
The charge-density distribution in 1H(+)-adeniniumtrichlorozinc(II) has been determined from X-ray diffraction data collected to sin theta/lambda = 1.32 A(-1) at 123 K. The electrostatic potential, isolated from the crystal lattice, and the...
Probing the limits of the molecular replacement method: the case of Trypanosoma brucei phosphoglycerate kinase [0.03%]
分子置换法在别构酶结构测定中的局限性——以布氏锥虫磷酸甘油酸激酶为例
B E Bernstein,W G Hol
B E Bernstein
It is of general interest to explore the limits of the molecular replacement method. Described here are the specifics of a successful molecular replacement structure determination in a difficult case: the X-ray crystal structure of a Trypan...