Crystallization and preliminary X-ray diffraction studies of UP1, the two-RRM domain of hnRNP A1 [0.03%]
UP1（hnRNP A1的两个RRM结构域）的结晶及初步X射线衍射研究
L Jokan,A P Dong,A Mayeda et al.
L Jokan et al.
The N-terminal domain of hnRNP A1 protein, termed UP1, comprises two tandem RNA-recognition motifs, both of which are necessary for efficient RNA binding and for the alternative splicing activity of hnRNP A1. Recombinant human UPI expressed...
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi [0.03%]
色氨酸酮酸菌的香草酸激酶的结晶及初步X射线晶体学分析
T Krell,J E Coyle,M J Horsburgh et al.
T Krell et al.
Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kin...
Crystallization and preliminary X-ray analysis of the thermostable alkaline-tolerant xylanase from Bacillus stearothermophilus T-6 [0.03%]
来自Bacillus stearothermophilus T-6的耐热碱性木聚糖酶的结晶及初步X射线分析
A Teplitsky,H Feinberg,R Gilboa et al.
A Teplitsky et al.
The extracellular thermostable xylanase (XT-6) produced by the thermophilic bacterium Bacillus stearothermophilus T-6 was shown to bleach pulp optimally at pH 9 and 338 K, and was successfully used in a large-scale biobleaching mill trial. ...
Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus [0.03%]
来自Coprinus cinereus的漆酶的结晶及初步X射线分析研究
V Ducros,G J Davies,D M Lawson et al.
V Ducros et al.
The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hangi...
Crystallization and preliminary X-ray analysis of the major endoglucanase from Thermoascus aurantiacus [0.03%]
来源于Thermoascus aurantiacus的主要内切-葡聚糖酶的结晶及初步X射线分析
L Lo Leggio,N J Parry,J Van Beeumen et al.
L Lo Leggio et al.
The major endoglucanase (35 kDa) from the thermophilic fungus Thermoascus aurantiacus has been purified from culture filtrates using an affinity method and the sequence for 35 N-terminal amino acids determined. This has allowed assignment o...
Crystallization and preliminary X-ray diffraction studies of the Pseudomonas marginata esterase EstB [0.03%]
产绿针孢菌酯酶EstB的结晶及初步晶体学研究
U G Wagner,B Sölkner,E I Petersen et al.
U G Wagner et al.
Crystals of the esterase EstB were obtained at 277 K with the hanging-drop technique from polyethylene glycol 4000 solutions containing 2-propanol at pH 7.5. The crystals belong to the trigonal space group P3(1)21 (or P3(2)21) with cell dim...
X-ray topography of tetragonal lysozyme grown by the temperature-controlled technique [0.03%]
由控温法生长的四方棱菌溶蛋白的x射线 topo 照相研究
V Stojanoff,D P Siddons,L A Monaco et al.
V Stojanoff et al.
Growth-induced defects in lysozyme crystals were observed by white-beam and monochromatic X-ray topography at the National Synchrotron Light Source (NSLS) at the Brookhaven National Laboratory (BNL). The topographic methods were non-destruc...
Comparison of the structures of the cubic and tetragonal forms of horse-spleen apoferritin [0.03%]
马脾清蛋白 ferritin 立方型和四方型结构的比较
T Granier,B Gallois,A Dautant et al.
T Granier et al.
Horse-spleen apoferritin is known to crystallize in three different space groups, cubic F432, tetragonal P42(1)2 and orthorhombic P2(1)2(1)2. A structure comparison of the cubic and tetragonal forms is presented here. Both crystal forms wer...
T C Terwilliger,J Berendzen
T C Terwilliger
A Bayesian treatment for phase calculation in the multiwavelength anomalous diffraction (MAD) technique is presented. This approach explicitly treats effects of errors correlated among measurements at different wavelengths and between Bijvo...
Ligand-induced conformational changes in poliovirus-antiviral drug complexes [0.03%]
多萝病毒-抗病毒药物复合物的配体诱导构象变化
C N Hiremath,D J Filman,R A Grant et al.
C N Hiremath et al.
Crystal structures of the Mahoney strain of type 1 poliovirus complexed with the antiviral compounds R80633 and R77975 were determined at 2.9 A resolution. These compounds block infection by preventing conformational changes required for vi...