R Coulombe,M Cygler
R Coulombe
Density-modification algorithms have gained, in recent years, a widespread use in the early stages of protein structure determination, especially in combination with the single isomorphous replacement, the multiple isomorphous replacement a...
M H Dung,J A Bell
M H Dung
The X-ray structure of crystal form IX of bovine pancre- atic ribonuclease A (space group P2(1)2(1)2(1)) is reported at 1.6 A resolution. The structure was refined to an R factor of 15.0% and includes coordinates for two sulfate ions, four ...
Structures of a blue-copper nitrite reductase and its substrate-bound complex [0.03%]
一种蓝色铜硝酸盐还原酶及其底物结合复合体的结构研究
F E Dodd,S S Hasnain,Z H Abraham et al.
F E Dodd et al.
Copper-containing nitrite reductases (NiR's) have been conveniently subdivided into blue and green NiR's which are thought to be redox partners of azurins and pseudo-azurins, respectively. Crystal structures of two green NiR's have recently...
Structure of rabbit muscle phosphoglucomutase refined at 2.4 A resolution [0.03%]
兔肌肉葡萄糖磷酸互变酶的结构 refinement 至 2.4A 分辨率
Y Liu,W J Ray Jr,S Baranidharan
Y Liu
Data between 6.0 and 2.4 A resolution, collected at 253 K, wer used to refine a revised atomic model of muscle phosphoglucomutase: final crystallographic R factor = 16.3% (Rfree = 19.1%); final r.m.s. deviations from ideal bond lengths and ...
Enhanced diffractivity of phosphoglucomutase crystals. Use of an alternative cryocrystallographic procedure [0.03%]
磷酸葡萄糖变位酶晶体的衍射增强。一种替代的冷冻结晶学方法的应用
W J Ray Jr,S Baranidharan,Y Liu
W J Ray Jr
A continuous procedure for replacing the 2 M (NH(4))(2)SO(4) within crystals of rabbit muscle phosphoglucomutase by 55% polyethylene glycol 600 (PEG 600) is described. The success rate (absence of fracturing) approaches 100% in spite of the...
Accuracy of determination of position and width of molecular groups in biological and lipid membranes via neutron diffraction [0.03%]
基于中子衍射的生物膜和脂质膜分子团的位置及宽度测定精度研究
V I Gordeliy,N I Chernov
V I Gordeliy
Neutron diffraction combined with the deuterium-labelled molecular groups of biological and model membrane components allows one to detect with high accuracy the structure of these objects. Experiments of this kind are only possible at uniq...
Bias reduction in phase refinement by modified interference functions: introducing the gamma correction [0.03%]
改良干涉函数在相位完善中的偏置降低：引入γ校正
J P Abrahams
J P Abrahams
The chemical, physical and symmetry constraints of an electron-density map impose relationships between structure factors, and these relationships are exploited during refinement. However, constraints often allow an artificially high correl...
R Li,L Chen,D Cai et al.
R Li et al.
The copper-containing amine oxidase from the yeast Hansenula polymorpha (YAO) has been crystallized and partially solved by molecular replacement. It catalyzes the oxidative deamination of primary amines by molecular oxygen to the correspon...
Structure of the calcium-binding echidna milk lysozyme at 1.9 A resolution [0.03%]
一种具有钙结合位点的鸭嘴兽乳汁溶菌酶的晶体结构（1.9Å分辨率）
J M Guss,M Messer,M Costello et al.
J M Guss et al.
A lysozyme isolated from the milk of a monotreme, the echidna, Tachyglossus aculeatus multiaculeatus, has been crystallized (space group P2(1), with unit-cell dimensions a = 37.1, b = 42.0, c = 38.1 A, beta = 91 degrees and Z = 2) and the s...
Crystallization and preliminary diffraction studies of morphinone reductase, a flavoprotein involved in the degradation of morphine alkaloids [0.03%]
吗啡生物碱降解酶摩尔酚酮还原酶的结晶及初步衍射研究
P C Moody,N Shikotra,C E French et al.
P C Moody et al.
Morphinone reductase from Pseudomonas putida M10, a flavoprotein involved in the degradation of morphine alkaloids, was purified from an overexpressing strain of Escherichia coli and crystallized using the hanging-drop vapour-diffusion meth...