Purification, crystallization and preliminary X-ray diffraction studies of recombinant calcium-binding domain of the small subunit of porcine calpain [0.03%]
钙调素小亚基钙结合域的纯化、结晶及初步X射线衍射研究
G D Lin,D Chattopadhyay,M Maki et al.
G D Lin et al.
The calcium-binding domain of the small subunit of porcine calpain (domain VI) has been expressed in Escherichia coli, purified, and crystallized in the presence of Ca(2+). Two crystal forms have been obtained by the vapor-diffusion method ...
Crystallization of the Fab fragment of the tumour-specific antibody PR1A3 [0.03%]
肿瘤特异性抗体PR1A3的Fab片段的结晶化研究
P Dokurno,J M Lally,P A Bates et al.
P Dokurno et al.
PR1A3 antibody binds specifically to the tumour-associated cell-surface antigen, carcinoembryonic antigen. Crystals of the Fab fragment of the PR1A3 antibody were obtained by vapour diffusion against mother liquor containing Tris-HC1 buffer...
Homology between jacalin and artocarpin from jackfruit (Artocarpus integrifolia) seeds. Partial sequence and preliminary crystallographic studies of artocarpin [0.03%]
辣木种子的Jacalin和Artocarpin之间的同源性。Artocarpin的部分序列及初步晶体学研究
S Suresh,P G Rani,J V Pratap et al.
S Suresh et al.
Jacalin and artocarpin, the two lectins from jackfruit (Artocarpus integrifolia) seeds, have different physicochemical properties and carbohydrate-binding specificities. However, comparison of the partial amino-acid sequence of artocarpin w...
Structure of apo duck ovotransferrin: the structures of the N and C lobes are in the open form [0.03%]
无鸭卵转铁蛋白(apo duck ovotransferrin)的结构:其N、C二个lobe均为开放型构象
A Rawas,H Muirhead,J Williams
A Rawas
The structure of apo duck ovotransferrin (APODOT) has been determined at a resolution of 4.0 A by the molecular replacement method using the structure of duck ovotransferrin (DOT) as the search model. The DOT structure contains two iron bin...
Crystallization and preliminary X-ray crystallographic analysis of the electron-transferring flavoprotein from Megasphaera elsdenii [0.03%]
来自Megasphaera elsdenii的电子转移黄素蛋白的结晶及初步X射线晶体学分析
C T Sharkey,M A Walsh,S G Mayhew et al.
C T Sharkey et al.
Electron-transferring flavoprotein from the rumen bacterium Megasphaera elsdenii is a heterodimer (M(r) = 75 kDa) containing FAD as cofactor and functioning solely to mediate electron transfer between the prosthetic groups of other proteins...
Crystallization and preliminary X-ray crystallographic studies of type A influenza virus matrix protein M1 [0.03%]
A型流感病毒基质蛋白M1的结晶及初步X射线晶体学研究
B Sha,M Luo
B Sha
The matrix protein, M1, of influenza virus strain A/PR/8/34 has been purified from virions and crystallized. The crystals consist of a stable fragment (18 kDa) of the M1 protein. X-ray diffraction studies indicated that the crystals are in ...
A M Holmes,S G Holliday,J C Clunie et al.
A M Holmes et al.
Using free-solution capillary electrophoresis, the electrophoretic mobility of micro m-sized lysozyme crystals in their growth solution at 283 K, 1.5%(w/v) NaC1, and over a range of pH values between 3.59 and 5.70 has been measured. Under t...
wARP: improvement and extension of crystallographic phases by weighted averaging of multiple-refined dummy atomic models [0.03%]
通过多重精修虚拟原子模型的加权平均改进和扩展晶体学相位研究
A Perrakis,T K Sixma,K S Wilson et al.
A Perrakis et al.
wARP is a procedure that substantially improves crystallographic phases (and subsequently electron-density maps) as an additional step after density-modification methods such as solvent flattening and averaging. The initial phase set is use...
Different tools to study interaction potentials in gamma-crystallin solutions: relevance to crystal growth [0.03%]
研究γ-结晶素溶液中相互作用势的不同工具：对晶体生长的意义
F Bonneté,M Malfois,S Finet et al.
F Bonneté et al.
Osmotic pressure, small-angle X-ray scattering and quasi-elastic light scattering were used to study the medium-range interaction potentials between macromolecules in solution. These potentials determine macromolecular crystallization. Calf...
A connected set algorithm for the identification of spatially contiguous regions in crystallographic envelopes [0.03%]
用于晶体学包络中识别空间连续区域的连接集算法
J F Hunt,F M Vellieux,J Deisenhofer
J F Hunt
A simple algorithm is described for the identification of spatially contiguous regions in crystallographic envelopes. In a single pass through the grid points of the envelope map, the occupied points are assigned to a series of locally cont...