Structures of Mycobacterium tuberculosis folylpolyglutamate synthase complexed with ADP and AMPPCP [0.03%]
结核分支杆菌叶酸多谷氨酰胺合成酶与ADP和AMPPCP复合物的晶体结构研究
Paul G Young,Clyde A Smith,Peter Metcalf et al.
Paul G Young et al.
Folate derivatives are essential vitamins for cell growth and replication, primarily because of their central role in reactions of one-carbon metabolism. Folates require polyglutamation to be efficiently retained within the cell and folate-...
A general method for phasing novel complex RNA crystal structures without heavy-atom derivatives [0.03%]
不用重原子衍生物解析复杂RNA晶体结构的一般方法
Michael P Robertson,William G Scott
Michael P Robertson
The crystallographic phase problem [Muirhead & Perutz (1963), Nature (London), 199, 633-638] remains the single major impediment to obtaining a three-dimensional structure of a macromolecule once suitable crystals have been obtained. Recent...
Structure and sugar-specificity of basic winged-bean lectin: structures of new disaccharide complexes and a comparative study with other known disaccharide complexes of the lectin [0.03%]
基本刀豆素与糖特异性：新双糖配基复合物的结构及与其他已知双糖复合物的比较研究
Kiran A Kulkarni,Samiksha Katiyar,Avadhesha Surolia et al.
Kiran A Kulkarni et al.
Crystal structures of the complexes of basic winged-bean agglutinin with the disaccharides Galalpha1-4Gal (galabiose), Galalpha1-6Glc (mellibiose) and Galalpha1-4Galbeta-Et have been determined and the complex with Galalpha1-2Gal has been m...
Exploiting the anisotropy of anomalous scattering boosts the phasing power of SAD and MAD experiments [0.03%]
利用异常散射的各向异性可增强SAD和MAD实验的相位提取能力
Marc Schiltz,Gérard Bricogne
Marc Schiltz
The X-ray polarization anisotropy of anomalous scattering in crystals of brominated nucleic acids and selenated proteins is shown to have significant effects on the diffraction data collected at an absorption edge. For conventionally collec...
Structural basis for the high-affinity binding of pyrrolotriazine inhibitors of p38 MAP kinase [0.03%]
吡咯三嗪p38丝裂原活化蛋白激酶抑制剂高亲和力结合的结构基础
John S Sack,Kevin F Kish,Matthew Pokross et al.
John S Sack et al.
The crystal structure of unphosphorylated p38alpha MAP kinase complexed with a representative pyrrolotriazine-based inhibitor led to the elucidation of the high-affinity binding mode of this class of compounds at the ATP-binding site. The l...
Structural characterization of a human Fc fragment engineered for lack of effector functions [0.03%]
工程改造后缺乏效应功能的人Fc片段的结构表征
Vaheh Oganesyan,Changshou Gao,Lena Shirinian et al.
Vaheh Oganesyan et al.
The first three-dimensional structure of a human Fc fragment genetically engineered for the elimination of its ability to mediate antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity is reported. When introduc...
Haruhiko Sakuraba,Kazunari Yoneda,Kiyo Takeuchi et al.
Haruhiko Sakuraba et al.
The crystal structure of a novel alanine:glyoxylate aminotransferase from the hyperthermophilic archaeon Thermococcus litoralis was determined at 2.3 A resolution. The asymmetric unit contains four homologous subunits and the functional tet...
Nucleant-mediated protein crystallization with the application of microporous synthetic zeolites [0.03%]
微孔合成分子筛在核子介导蛋白质结晶中的应用
Michihiro Sugahara,Yukuhiko Asada,Yuko Morikawa et al.
Michihiro Sugahara et al.
Protein crystallization is still a major bottleneck in structural biology. As the current methodology of protein crystallization is a type of screening, it is usually difficult to crystallize important target proteins. It was thought that h...
Insights into the structural basis of substrate recognition by histidinol-phosphate aminotransferase from Corynebacterium glutamicum [0.03%]
大谷氨酸菌组氨醇-磷酸转氨酶底物识别的结构基础研究
Jan Marienhagen,Tatyana Sandalova,Hermann Sahm et al.
Jan Marienhagen et al.
Histidinol-phosphate aminotransferase (HisC) is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the reversible transamination reaction between histidinol phosphate (His-P) and 2-oxoglutarate (O-Glu). The crystal structures of apo h...
Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 A resolution [0.03%]
硫化叶菌杂聚簇蛋白的结构与功能关系：Desulfovibrio vulgaris杂聚簇蛋白的厌氧纯化结构（解析精度为1.35埃）
David Aragão,Edward P Mitchell,Carlos F Frazão et al.
David Aragão et al.
The hybrid cluster protein (HCP) from the sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough has been isolated and crystallized anaerobically. The phase problem was solved for a P2(1)2(1)2(1) crystal form using multiple-...