Interpretation of ensembles created by multiple iterative rebuilding of macromolecular models [0.03%]
基于大分子模型多次迭代重建的系综的解读
Thomas C Terwilliger,Ralf W Grosse-Kunstleve,Pavel V Afonine et al.
Thomas C Terwilliger et al.
Automation of iterative model building, density modification and refinement in macromolecular crystallography has made it feasible to carry out this entire process multiple times. By using different random seeds in the process, a number of ...
Structure of a CBS-domain pair from the regulatory gamma1 subunit of human AMPK in complex with AMP and ZMP [0.03%]
与AMP和ZMP结合的人AMPK调节亚基γ1上CBS二聚体的结构
Philip Day,Andrew Sharff,Lina Parra et al.
Philip Day et al.
AMP-activated kinase (AMPK) is central to sensing energy status in eukaryotic cells via binding of AMP and ATP to CBS (cystathionine beta-synthase) domains in the regulatory gamma subunit. The structure of a CBS-domain pair from human AMPK ...
Structure of the equine arteritis virus nucleocapsid protein reveals a dimer-dimer arrangement [0.03%]
马动脉炎病毒核衣壳蛋白结构显示由二聚体-二聚体组成
Ashlesha Deshpande,Sifang Wang,Martin A Walsh et al.
Ashlesha Deshpande et al.
Equine arteritis virus (EAV) is an enveloped positive-sense RNA virus belonging to the Arteriviridae family, which also includes the porcine pathogen PRRSV and is genetically and structurally related to the coronaviruses. EAV is an importan...
Alexandra Samal,Norbert Schormann,William J Cook et al.
Alexandra Samal et al.
Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate in the presence of Mg(2+) ions. The enzyme plays multiple cellular roles by maintaining a low dUTP:dTTP ratio and by synthesi...
Dilyana G Georgieva,Maxim E Kuil,Tjerk H Oosterkamp et al.
Dilyana G Georgieva et al.
Nucleation is the rate-limiting step in protein crystallization. Introducing heterogeneous substrates may in some cases lower the energy barrier for nucleation and thereby facilitate crystal growth. To date, the mechanism of heterogeneous p...
Structure of the mexicain-E-64 complex and comparison with other cysteine proteases of the papain family [0.03%]
墨西哥菌素-E-64复合物的结构及其与其它半胱氨酸蛋白酶（木瓜素家族）的比较
J A Gavira,L A González-Ramírez,M C Oliver-Salvador et al.
J A Gavira et al.
Mexicain is a 23.8 kDa cysteine protease from the tropical plant Jacaratia mexicana. It is isolated as the most abundant product after cation-exchange chromatography of the mix of proteases extracted from the latex of the fruit. The purifie...
An automated microseed matrix-screening method for protein crystallization [0.03%]
蛋白质结晶的自动化微种子矩阵筛选方法
Allan D&#x;Arcy,Frederic Villard,May Marsh
Allan D&#x;Arcy
A microseed-matrix procedure has been established with the aim of influencing the nucleation event in standard crystallization screens. The method is based on the original description of matrix seeding described by Ireton & Stoddard (2004, ...
An alternate description of two crystal structures of phospholipase A2 from Bungarus caeruleus [0.03%]
学名Bungarus caeruleus的磷脂酶A2的两种晶体结构的一个替代描述
Isolde Le Trong,Ronald E Stenkamp
Isolde Le Trong
Reinterpretations of the space-group symmetry are reported for two crystal structures of phospholipase A(2) isoforms (PDB codes 1u4j and 1g2x). The two structures reported in space groups R3 and C2 are isomorphous with a third isoform with ...
Structures of and interactions between domains of trigger factor from Thermotoga maritima [0.03%]
来自Thermotoga maritima的引发因子结构域的结构和相互作用
Erik Martinez-Hackert,Wayne A Hendrickson
Erik Martinez-Hackert
Trigger factor (TF) is a eubacterial chaperone that associates with ribosomes at the peptide-exit tunnel and also occurs in excess free in the cytosol. TF is a three-domain protein that appears to exist in a dynamic equilibrium of oligomeri...
A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye [0.03%]
一种通用的蛋白质晶体鉴别方法：在结晶实验中使用非共价荧光染料识别蛋白质晶体的方法
Matthew R Groves,Ingrid B Müller,Xandra Kreplin et al.
Matthew R Groves et al.
A technique is described whereby the addition of low concentrations (millimolar to micromolar) of the fluorescent dye 1,8-ANS to the protein solution prior to crystallization results in crystallization experiments in which protein crystals ...