Structure of a d(TGGGGT) quadruplex crystallized in the presence of Li+ ions [0.03%]
锂离子存在下结晶的d(TGGGGT)四链体结构
Christophe Creze,Bruno Rinaldi,Richard Haser et al.
Christophe Creze et al.
A parallel 5'-d(TGGGGT)-3' quadruplex was formed in Na(+) solution and crystallized using lithium sulfate as the main precipitating agent. The X-ray structure was determined to 1.5 A resolution in space group P2(1) by molecular replacement....
The structure of a d(gcGAACgc) duplex containing two consecutive bulged A residues in both strands suggests a molecular switch [0.03%]
含有连续突出的A残基的双链体d(gcGAACgc)的结构表明这是一种分子开关
Jiro Kondo,Tomoko Sunami,Akio Takénaka
Jiro Kondo
In previous studies, it was reported that DNA fragments with the sequence d(gcGXYAgc) (where X = A or G and Y = A, T or G) form a stable base-intercalated duplex (Bi-duplex) in which the central X and Y residues are not involved in any base...
The atomic resolution structure of human aldose reductase reveals that rearrangement of a bound ligand allows the opening of the safety-belt loop [0.03%]
人源己糖还原酶的原子结构揭示了结合配体的重排使得安全带环张开
Marianna Biadene,Isabelle Hazemann,Alexandra Cousido et al.
Marianna Biadene et al.
The crystal structure of human aldose reductase in complex with citrate has been determined to a resolution of 0.82 A. The difference electron density for H atoms unequivocally shows that the cofactor is in the oxidized state corresponding ...
Notes of a protein crystallographer: quo vadis structural biology? [0.03%]
一个蛋白质晶体学家的笔记——结构生物学的未来在哪里？
Cele Abad-Zapatero
Cele Abad-Zapatero
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths [0.03%]
高压低温装置用于毛细管样品保护及长波长衍射相位实验
Chae Un Kim,Quan Hao,Sol M Gruner
Chae Un Kim
Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of pene...
Crystallization by capillary counter-diffusion and structure determination of the N114A mutant of the SH3 domain of Abl tyrosine kinase complexed with a high-affinity peptide ligand [0.03%]
毛细管反向扩散结晶及与高亲和力多肽配基结合的Src同源区3结构域复合物晶体结构测定(Abl酪氨酸激酶N114A变异体)
Ana Cámara-Artigas,Andrés Palencia,José C Martínez et al.
Ana Cámara-Artigas et al.
The recognition of proline-rich ligands by SH3 domains is part of the process leading to diseases such as cancer or AIDS. Understanding the molecular determinants of the binding affinity and specificity of these interactions is crucial for ...
Protein crystallization by surface entropy reduction: optimization of the SER strategy [0.03%]
表面熵减法蛋白质结晶及其技术优化
David R Cooper,Tomasz Boczek,Katarzyna Grelewska et al.
David R Cooper et al.
A strategy of rationally engineering protein surfaces with the aim of obtaining mutants that are distinctly more susceptible to crystallization than the wild-type protein has previously been suggested. The strategy relies on replacing small...
A Rac1-GDP trimer complex binds zinc with tetrahedral and octahedral coordination, displacing magnesium [0.03%]
Rac1-GDP三元复合物的四面体和八面体配位结合锌，置换镁
Gerd Prehna,C Erec Stebbins
Gerd Prehna
The Rho family of small GTPases represent well characterized signaling molecules that regulate many cellular functions such as actin cytoskeletal arrangement and the cell cycle by acting as molecular switches. A Rac1-GDP-Zn complex has been...
Probing the oxygen-binding site of the human formylglycine-generating enzyme using halide ions [0.03%]
使用卤化物离子探测人醛甘氨酸生成酶的氧结合位点
Dirk Roeser,Bernhard Schmidt,Andrea Preusser-Kunze et al.
Dirk Roeser et al.
The catalytic residue in sulfatases is a unique formylglycine that is post-translationally generated by oxidation of a cysteine or serine precursor. Molecular oxygen oxidizes the cysteine precursor in eukaryotic sulfatases, a reaction that ...
Stereochemical restraints revisited: how accurate are refinement targets and how much should protein structures be allowed to deviate from them? [0.03%]
stereo化学约束再探讨：精修目标多准确？允许蛋白质结构偏离这些目标的程度有多大？
Mariusz Jaskolski,Miroslaw Gilski,Zbigniew Dauter et al.
Mariusz Jaskolski et al.
The Protein Data Bank and Cambridge Structural Database were analyzed with the aim of verifying whether the restraints that are most commonly used for protein structure refinement are still appropriate 15 years after their introduction. Fro...