Using X-ray absorption spectra to monitor specific radiation damage to anomalously scattering atoms in macromolecular crystallography [0.03%]
利用X射线吸收谱监测大型分子晶体学中异常散射原子的特定辐射损伤
V Oliéric,E Ennifar,A Meents et al.
V Oliéric et al.
Radiation damage in macromolecular crystals is not suppressed even at 90 K. This is particularly true for covalent bonds involving an anomalous scatterer (such as bromine) at the 'peak wavelength'. It is shown that a series of absorption sp...
What happens when the signs of anomalous differences or the handedness of substructure are inverted? [0.03%]
当反常差热或子结构的手性反转时会发生什么情况？
Jiawei Wang,Alexander Wlodawer,Zbigniew Dauter
Jiawei Wang
Proper solution of a macromolecular crystal structure based on anomalous scattering and/or isomorphous differences requires that the anomalous differences in reflection amplitudes be measured properly and that the correct enantiomer of the ...
Pseudo-merohedral twinning in monoclinic crystals of human orotidine-5'-monophosphate decarboxylase [0.03%]
人鸟苷-5'-磷酸脱羧酶单斜晶系伪似对称孪生现象
Julia G Wittmann,Markus G Rudolph
Julia G Wittmann
Human UMP synthase is a bifunctional enzyme that catalyzes the penultimate and last steps in the de novo biosynthesis of UMP. In contrast to prokaryotes, UMP synthase from higher eukaryotes combines the orotate phosphoribosyltransferase and...
Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution [0.03%]
人β-半乳糖苷酶基因的克隆、表达及改造菌株发酵条件优化研究
Mika K Rantanen,Lari Lehtiö,Lakshmi Rajagopal et al.
Mika K Rantanen et al.
Streptococcus agalactiae, a prokaryote that causes infections in neonates and immunocompromised adults, has a serine/threonine protein kinase (STK) signalling cascade. The structure of one of the targets, a family II inorganic pyrophosphata...
Structure of human upstream binding factor HMG box 5 and site for binding of the cell-cycle regulatory factor TAF1 [0.03%]
人上游启动因子HMG框5的结构及其与细胞周期调节因子TAF1结合位点的位置关系
Hui Rong,Yan Li,Xiaowei Shi et al.
Hui Rong et al.
The fifth HMG-box domain in human upstream binding factor (hUBF) contributes to the synthesis of rRNA by RNA polymerase I (Pol I). The 2.0 A resolution crystal structure of this protein has been solved using the single-wavelength anomalous ...
Structural and pharmacological comparison of daboiatoxin from Daboia russelli siamensis with viperotoxin F and vipoxin from other vipers [0.03%]
从其他金环蛇中获得的daboiatoxin与viperotoxin F和vipoxin的结构及药理学比较研究
Gayathri Gopalan,Maung-Maung Thwin,Ponnampalam Gopalakrishnakone et al.
Gayathri Gopalan et al.
Russell's viper (Vipera russelli, also known as Daboia russelli) is one of the major causes of fatal snakebites. To date, five Daboia russelli subspecies have been recognized. Daboiatoxin (DbTx) is the main lethal phospholipase A(2) (PLA(2)...
Crystal structure of CD59: implications for molecular recognition of the complement proteins C8 and C9 in the membrane-attack complex [0.03%]
CD59的晶体结构及其对补体蛋白C8和C9在膜攻击复合物中分子识别的意义
Yuxiang Huang,Alena Fedarovich,Stephen Tomlinson et al.
Yuxiang Huang et al.
Human CD59 is a small membrane-bound glycoprotein that functions as an inhibitor of the membrane-attack complex (MAC) of the complement system by binding the complement proteins C8 and C9. The crystal structure of a soluble construct of CD5...
On the application of molecular-dynamics simulations to validate thermal parameters and to optimize TLS-group selection for macromolecular refinement [0.03%]
分子动力学模拟在验证热参数以及优化大分子 refine 中 tls 组选择的应用
Nicholas M Glykos
Nicholas M Glykos
Comparison of crystallographically determined and molecular dynamics simulation-derived parameters for a small (26 kDa) homotetrameric four-alpha-helical bundle protein revealed an unexpected pattern of similarities and differences between ...
Structure-assisted discovery of Variola major H1 phosphatase inhibitors [0.03%]
结构辅助的大痘病毒H1蛋白磷酸酶抑制剂的发现
Jason Phan,Joseph E Tropea,David S Waugh
Jason Phan
Variola major virus, the causative agent of smallpox, encodes the dual-specificity H1 phosphatase. Because this enzyme is essential for the production of mature virus particles, it is an attractive molecular target for the development of th...
Probing the S2 site of factor VIIa to generate potent and selective inhibitors: the structure of BCX-3607 in complex with tissue factor-factor VIIa [0.03%]
探寻FVIIa的S2结合位点以生成强有力的特异性抑制剂：BCX-3607与组织因子-FVIIa复合物的晶体结构分析
Raman Krishnan,Pravin L Kotian,Pooran Chand et al.
Raman Krishnan et al.
Factor VIIa (FVIIa) is a trypsin-like serine protease in the coagulation cascade. Its complex with tissue factor (TF) triggers the extrinsic pathway of the coagulation cascade, generating a blood clot. Research programs at several centers n...