Conformational flexibility in the flap domains of ligand-free HIV protease [0.03%]
无配体HIV蛋白酶底物夹具结构域的构象灵活性
Holly Heaslet,Robin Rosenfeld,Mike Giffin et al.
Holly Heaslet et al.
The crystal structures of wild-type HIV protease (HIV PR) in the absence of substrate or inhibitor in two related crystal forms at 1.4 and 2.15 A resolution are reported. In one crystal form HIV PR adopts an 'open' conformation with a 7.7 A...
The effect of heavy atoms on the conformation of the active-site polypeptide loop in human ABO(H) blood-group glycosyltransferase B [0.03%]
重原子对人ABO(H)血型糖基转移酶B活性部位多肽环结构的影响
James A Letts,Mattias Persson,Brock Schuman et al.
James A Letts et al.
The human ABO(H) blood-group antigens are oligosaccharide structures that are expressed on erythrocyte and other cell surfaces. The terminal carbohydrate residue differs between the blood types and determines the immune reactivity of this a...
Structure-specificity relationships of an intracellular xylanase from Geobacillus stearothermophilus [0.03%]
解淀粉芽孢杆菌内切-1,4-β-xylan酶结构与特异性的关系研究
V Solomon,A Teplitsky,S Shulami et al.
V Solomon et al.
Geobacillus stearothermophilus T-6 is a thermophilic Gram-positive bacterium that produces two selective family 10 xylanases which both take part in the complete degradation and utilization of the xylan polymer. The two xylanases exhibit si...
High-resolution structures of oxidized and reduced thioredoxin reductase from Helicobacter pylori [0.03%]
幽门螺旋杆菌氧化型和还原型硫氧还蛋白还原酶的高分辨率结构
Tomas N Gustafsson,Tatyana Sandalova,Jun Lu et al.
Tomas N Gustafsson et al.
The crystal structures of homodimeric thioredoxin reductase (TrxR) from the gastric pathogen Helicobacter pylori in complex with NADP(+) have been determined for the oxidized and reduced form of the enzyme at 1.7 and 1.45 A resolution, resp...
Initial evaluations of the reproducibility of vapor-diffusion crystallization [0.03%]
蒸气扩散结晶重复性的初步评估
Janet Newman,Jian Xu,Michael C Willis
Janet Newman
Experiments were set up to test how the crystallization drop size affects the crystallization process; in the test cases studied, increasing the drop size led to increasing numbers of crystals. Other data produced from a high-throughput aut...
A posteriori design of crystal contacts to improve the X-ray diffraction properties of a small RNA enzyme [0.03%]
事后设计晶体接触以改善一个小的RNA酶的X射线衍射性质
Celeste MacElrevey,Robert C Spitale,Jolanta Krucinska et al.
Celeste MacElrevey et al.
The hairpin ribozyme is a small catalytic RNA comprising two helix-loop-helix domains linked by a four-way helical junction (4WJ). In its most basic form, each domain can be formed independently and reconstituted without a 4WJ to yield an a...
Structure and stability of designed TPR protein superhelices: unusual crystal packing and implications for natural TPR proteins [0.03%]
设计的TPR蛋白超螺旋的结构与稳定性：非同寻常的晶体堆积及对天然TPR蛋白的意义
Tommi Kajander,Aitziber L Cortajarena,Simon Mochrie et al.
Tommi Kajander et al.
The structure and stability of repeat proteins has been little studied in comparison to the properties of the more familiar globular proteins. Here, the structure and stability of designed tetratricopeptide-repeat (TPR) proteins is describe...
Yao He,De-Qiang Yao,Yuan-Xin Gu et al.
Yao He et al.
A method of dual-space molecular-replacement model completion has been proposed which involves the programs ARP/wARP, REFMAC, OASIS and DM. OASIS is used in reciprocal space for phase refinement based on models built by ARP/wARP. For this p...
Bence Jones KWR protein structures determined by X-ray crystallography [0.03%]
通过X射线晶体学确定苯丙氨酸氨基转移酶KWR蛋白结构
Debora L Makino,Agnes H Henschen-Edman,Steven B Larson et al.
Debora L Makino et al.
A Bence Jones protein isolated in the early 1960s from a patient (initials KWR) suffering from plasma-cell dyscrasia was crystallized and its structure was analyzed in four different unit cells by X-ray diffraction. The final models of the ...
Structure of the small GTPase Rab27b shows an unexpected swapped dimer [0.03%]
小GTP酶Rab27b的结构显示了一个意外的交换二聚体构象
Leonard M G Chavas,Seiji Torii,Hironari Kamikubo et al.
Leonard M G Chavas et al.
Members of the Rab family of small GTPases regulate membrane traffic within the cell by recruiting their specific effectors in a nucleotide-dependent manner. The Rab27 subfamily consists of Rab27a and Rab27b, which share 70% sequence identi...