Alternative models for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase [0.03%]
卡利巴克霉菌3,4-二羟基-2-丁酮-4-磷酸合成酶两种晶体结构的替代模型
Isolde Le Trong,Ronald E Stenkamp
Isolde Le Trong
Reinterpretation of the space-group symmetry is reported for two crystal structures of Candida albicans 3,4-dihydroxy-2-butanone 4-phosphate synthase (PDB codes 1tks and 1tku). The two structures reported in space group P1 with a dimer in t...
High-resolution structure of methionine gamma-lyase from Citrobacter freundii [0.03%]
柠檬酸杆菌蛋氨酰胺酶的高分辨率结构
Alexei Nikulin,Svetlana Revtovich,Elena Morozova et al.
Alexei Nikulin et al.
Pyridoxal 5'-phosphate-dependent methionine gamma-lyase (MGL) is involved in the metabolism of sulfur-containing amino acids. The enzyme is a promising target in some anaerobic pathogens and is effective in cancer-cell treatment. The struct...
Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate [0.03%]
大肠杆菌外切酶I与胸腺嘧啶5'-单磷酸复合物的结构
Robert D Busam
Robert D Busam
In Escherichia coli, exonuclease I (ExoI) is a monomeric processive 3'-5' exonuclease that degrades single-stranded DNA. The enzyme has been implicated as primarily being involved in repairing frameshift mutations. The structure of the enzy...
Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor [0.03%]
链霉菌TetR家族转录调控蛋白SCO0332的结构与功能研究
Ui Okada,Kazuya Kondo,Takeshi Hayashi et al.
Ui Okada et al.
SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using the ...
In situ X-ray analysis of protein crystals in low-birefringent and X-ray transmissive plastic microchannels [0.03%]
低双折射率及射线透过性塑料微通道中蛋白质晶体的原位X射线分析
Joseph D Ng,Peter J Clark,Raymond C Stevens et al.
Joseph D Ng et al.
Plastic microchannel crystallization template designs made from inexpensive cyclic olefin copolymers have been shown to be low-birefringent, X-ray transmissive and compatible with microfluidic fabrication in restricted geometry. The model p...
Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM [0.03%]
结构研究显示丙糖磷酸异构酶（TIM）催化环6的C端铰链处A178L突变使二聚体和单体TIM呈现封闭型构象
Markus Alahuhta,Marco G Casteleijn,Peter Neubauer et al.
Markus Alahuhta et al.
The flexible catalytic loop, loop-6, of TIM has evolved to preferably be open in the unliganded state and to preferably be closed in the liganded state. The N-terminal and C-terminal hinges of this loop are important for its opening/closing...
Hongliang Xu,Charles M Weeks
Hongliang Xu
Direct methods of phase determination have played an important role in determining heavy-atom substructures from difference amplitudes of native-derivative crystal pairs or crystals containing anomalously scattering atoms. The minimal princ...
A novel method for detection of selenomethionine incorporation in protein crystals via Raman microscopy [0.03%]
一种通过拉曼显微镜检测蛋白质晶体中硒代甲硫氨酸掺入的新方法
Alessandro Vergara,Antonello Merlino,Elio Pizzo et al.
Alessandro Vergara et al.
Multiwavelength anomalous dispersion (MAD) is the most widespread approach in structural biology for the determination of the crystal structure of a novel protein. Mass spectrometry is currently used to evaluate the selenomethionine (SeMet)...
Protein crystallography with a micrometre-sized synchrotron-radiation beam [0.03%]
微米级同步辐射光束的蛋白质晶体学研究
Rouslan Moukhametzianov,Manfred Burghammer,Patricia C Edwards et al.
Rouslan Moukhametzianov et al.
For the first time, protein microcrystallography has been performed with a focused synchrotron-radiation beam of 1 microm using a goniometer with a sub-micrometre sphere of confusion. The crystal structure of xylanase II has been determined...
Structures of potent selective peptide mimetics bound to carboxypeptidase B [0.03%]
一类高效选择性肽模拟物与羧基蛋白酶B的复合物结构
Marc Adler,Brad Buckman,Judi Bryant et al.
Marc Adler et al.
This article reports the crystal structures of inhibitors of the functional form of thrombin-activatable fibrinolysis inhibitor (TAFIa). In vivo experiments indicate that selective inhibitors of TAFIa would be useful in the treatment of hea...