J S Wang,N Pavlotsky,A I Tauber et al.
J S Wang et al.
We have extended our previous studies of adherent neutrophils and compared actin depolymerization and intracellular calcium changes induced by adherence to laminin and fibronectin. In order to accurately assess cellular actin changes, F-act...
Neurofilaments move apart freely when released from the circumferential constraint of the axonal plasma membrane [0.03%]
轴突细胞膜周向约束释放时神经丝可以自由分开
A Brown,R J Lasek
A Brown
Squid giant axons were used to obtain axonal cytoskeletons that had been separated from the confines of their plasma membranes. To remove the plasma membrane, axoplasm was extruded from the giant axon directly into an artificial axoplasm so...
Contractile protein dynamics of myofibrils in paired adult rat cardiomyocytes [0.03%]
成年大鼠心肌细胞二联体中肌丝收缩蛋白的动态变化规律
K Imanaka-Yoshida,J M Sanger,J W Sanger
K Imanaka-Yoshida
The purpose of this study was to determine how quickly contractile proteins are incorporated into the myofibrils of freshly isolated cardiomyocytes and to determine whether there are regions of the cells that are more dynamic than others in...
Smooth muscle myosin subfragment-1 is a kinetic analogue for heavy meromyosin in the extended conformation [0.03%]
伸展构象下的肌动蛋白滑行：平滑肌肌球蛋白亚基1与重链杆肌球蛋白的动力学相似性研究
J S Drew,M P White,L A Stein
J S Drew
The 10S-->6S (Flexed-->Extended) transition in smooth muscle myosin is related to increased ATPase activity, but there is controversy over whether the analogous 9S-->7S transition in HMM is also associated with ATPase activity. We therefore...
Unambiguous classification of microtubule-ends in vitro: dynamic properties of the plus- and minus-ends [0.03%]
体外微管末端的明确分类：正端和负端的动力学性质
R J Kowalski,R C Williams Jr
R J Kowalski
To understand the mechanism of dynamic instability of microtubule growth and shortening, one needs a means of reliably determining the polarity of the microtubules under investigation. Sea urchin sperm-tail axonemal fragments nucleate the g...
L Cassimeris
L Cassimeris
Characterization of microtubule assembly in vivo and in vitro has raised questions of how cells regulate dynamic instability. While dynamic instability is an intrinsic property of the tubulin molecule, factors are required to increase the p...
K T Edds
K T Edds
Sea urchin coelomyocytes naturally flatten on a substratum into a discoid morphology and display striking, centripetally directed cortical flow along the radii of the cell when viewed with time lapse, video enhanced microscopy. The rate of ...
In vitro functional characterization of bacterially expressed human fibroblast tropomyosin isoforms and their chimeric mutants [0.03%]
人成纤维细胞异型体及其嵌合突变体的细菌表达及功能表征
R E Novy,J R Sellers,L F Liu et al.
R E Novy et al.
At least eight tropomyosin isoforms (hTM1, hTM2, hTM3, hTM4, hTM5, hTM5a, hTM5b, and hTMsm alpha) are expressed from four distinct genes in human fibroblasts. In order to elucidate isoform properties, we have subcloned hTM3 and hTM5 full-le...
Comparative Study
Cell motility and the cytoskeleton. 1993;26(3):248-61. DOI:10.1002/cm.970260308 1993
Centrosome repositioning immediately following karyokinesis and prior to cytokinesis [0.03%]
有丝分裂结束后细胞分裂前中心体重新定位至特定位置
G Mack,J B Rattner
G Mack
The behaviour of the centrosome immediately following cell division in tissue culture cells has been investigated. We find that following karyokinesis, but preceding cytokinesis, sister centrosomes relocate from the spindle poles to a posit...
K Onoda,F X Yu,H L Yin
K Onoda
gCap39 is a newly identified member of the Ca(2+)- and polyphosphoinositide-modulated gelsolin family of actin binding proteins which is different from gelsolin in several important respects: it caps filament ends, it does not sever filamen...