Twenty-five dyneins in Tetrahymena: A re-examination of the multidynein hypothesis [0.03%]
四膜虫中的二十五种动力蛋白：多重动力蛋白假说的再考察
David E Wilkes,Hadley E Watson,David R Mitchell et al.
David E Wilkes et al.
Dyneins are responsible for essential movements in eukaryotic cells. The motor activity of each dynein complex resides in its complement of heavy chains. In the present study, we examined 136 heavy chain sequences from the completed genomes...
Cell shape regulates collagen type I expression in human tendon fibroblasts [0.03%]
细胞形状调节人类腱纤维母细胞I型胶原表达
Fang Li,Bin Li,Qing-Ming Wang et al.
Fang Li et al.
Understanding the relationship between cell shape and cellular function is important for study of cell biology in general and for regulation of cell phenotype in tissue engineering in particular. In this study, microcontact printing techniq...
Molecular characterization and expression of a novel kinesin which localizes with the kinetoplast in the human pathogen, Leishmania donovani [0.03%]
人病原体利什曼原虫的新型动力蛋白分子特征及表达式式的鉴定与定位
Noel J Gerald,Isabelle Coppens,Dennis M Dwyer
Noel J Gerald
Using a variety of molecular and cell biological approaches, we identified, characterized and expressed a novel kinesin, LdK39B in the protozoan pathogen Leishmania donovani. Results of RT-PCR revealed two distinct LdK39B products with diff...
Changes in the magnitude and distribution of forces at different Dictyostelium developmental stages [0.03%]
变形虫不同发育阶段力量大小和分布的变化
H Delanoë-Ayari,S Iwaya,Y T Maeda et al.
H Delanoë-Ayari et al.
The distribution of forces exerted by migrating Dictyostelium amebae at different developmental stages was measured using traction force microscopy. By using very soft polyacrylamide substrates with a high fluorescent bead density, we could...
Axoneme-dependent tubulin modifications in singlet microtubules of the Drosophila sperm tail [0.03%]
果蝇精子尾部单管微管中的轴丝依赖性角蛋白修饰
Henry D Hoyle,F Rudolf Turner,Elizabeth C Raff
Henry D Hoyle
Drosophila melanogaster sperm tubulins are posttranslationally glutamylated and glycylated. We show here that axonemes are the substrate for these tubulin C-terminal modifications. Axoneme architecture is required, but full length, motile a...
Lan Lu,Yunfeng Feng,William J Hucker et al.
Lan Lu et al.
Actin stress fibers (SFs) enable cells to sense and respond to mechanical stimuli and affect adhesion, motility and apoptosis. We and others have demonstrated that cultured human aortic endothelial cells (HAECs) are internally stressed so t...
Molecular characterization of axonemal proteins and signaling molecules responsible for chemoattractant-induced sperm activation in Ciona intestinalis [0.03%]
碱性寡糖和信号分子诱导肠索氏居维叶氏虫精子激活的分子机理研究
Akiko Hozumi,Potturi Padma,Tosifusa Toda et al.
Akiko Hozumi et al.
Spermatozoa undergo dramatic physiological changes at fertilization. In the ascidian Ciona intestinalis, an egg-derived substance named SAAF induces both sperm activation and chemotaxis to the egg. To elucidate the molecular mechanism under...
Building a radial spoke: flagellar radial spoke protein 3 (RSP3) is a dimer [0.03%]
构建辐条：鞭毛辐条蛋白3(RSP3)是二聚体
Maureen Wirschell,Feifei Zhao,Chun Yang et al.
Maureen Wirschell et al.
Radial spokes are critical multisubunit structures required for normal ciliary and eukaryotic flagellar motility. Experimental evidence indicates the radial spokes are mechanochemical transducers that transmit signals from the central pair ...
Ellen M Popodi,Henry D Hoyle,F Rudolf Turner et al.
Ellen M Popodi et al.
The relationship between the primary structure of the beta-tubulin C-terminal tail (CTT) and axoneme structure and function is explored using the spermatogenesis-specific beta2-tubulin of Drosophila. We previously showed that all beta-tubul...
Single molecule polymerization, annealing and bundling dynamics of SipA induced actin filaments [0.03%]
SipA诱导的肌动蛋白丝的单分子聚合、退火和聚束动力学
David Popp,Akihiro Yamamoto,Mitsusada Iwasa et al.
David Popp et al.
Salmonella bacteria cause more than three million deaths each year. They hijack cells and inject among other proteins SipA via a "molecular syringe" into the cell, which can tether actin subunits in opposing strands to form mechanically sta...