The spindle pole body plays a key role in controlling mitotic commitment in the fission yeast Schizosaccharomyces pombe [0.03%]
芽体杆在裂殖酵母(Schizosaccharomyces pombe)有丝分裂承诺中起关键作用
Iain M Hagan
Iain M Hagan
Commitment to mitosis is regulated by a conserved protein kinase complex called MPF (mitosis-promoting factor). MPF activation triggers a positive-feedback loop that further promotes the activity of its activating phosphatase Cdc25 and is a...
Samuel Marguerat,Brian T Wilhelm,Jürg Bähler
Samuel Marguerat
The development of DNA sequencing more than 30 years ago has profoundly impacted biological research. In the last couple of years, remarkable technological innovations have emerged that allow the direct and cost-effective sequencing of comp...
Post-transcriptional regulation of gene expression in response to iron deficiency: co-ordinated metabolic reprogramming by yeast mRNA-binding proteins [0.03%]
铁缺乏条件下酵母mRNA结合蛋白对基因表达的表观遗传调控及代谢重组功能研究
Sandra V Vergara,Dennis J Thiele
Sandra V Vergara
Saccharomyces cerevisiae (baker's yeast) is an excellent model for understanding fundamental biological mechanisms that are conserved in Nature and that have an impact on human disease. The metal iron is a redox-active cofactor that plays c...
Mick Tuite,Klement Stojanovski,Frederique Ness et al.
Mick Tuite et al.
Prions represent an unusual structural form of a protein that is 'infectious'. In mammals, prions are associated with fatal neurodegenerative diseases such as CJD (Creutzfeldt-Jakob disease), while in fungi they act as novel epigenetic regu...
Mass spectrometric analysis of HOCl- and free-radical-induced damage to lipids and proteins [0.03%]
HOCl和自由基对脂质和蛋白质损伤的质谱分析
Andrew R Pitt,Corinne M Spickett
Andrew R Pitt
In inflammatory diseases, release of oxidants leads to oxidative damage to biomolecules. HOCl (hypochlorous acid), released by the myeloperoxidase/H2O2/Cl- system, can cause formation of phospholipid chlorohydrins, or alpha-chloro-fatty ald...
Steffen Loft,Pernille Høgh Danielsen,Lone Mikkelsen et al.
Steffen Loft et al.
Oxidative-stress-induced damage to DNA includes a multitude of lesions, many of which are mutagenic and have multiple roles in cancer and aging. Many lesions have been characterized by MS-based methods after extraction and digestion of DNA....
John K Lodge
John K Lodge
Vitamin E is an important nutrient with antioxidant and non-antioxidant functions, and certain evidence suggests that it has a cardiovascular protective role. It is therefore important to maintain an optimal vitamin E status. In the present...
Jaffar Nourooz-Zadeh
Jaffar Nourooz-Zadeh
A large body of evidence indicates that measurement of F2-isoprostanes, specific prostaglandin F2-like compounds derived from the non-enzymatic peroxidation of arachidonic acid, is a reliable biomarker of oxidant stress in the human body. S...
Analysis of eicosanoids and related lipid mediators using mass spectrometry [0.03%]
质谱法分析二十烷酸及相关脂类介质
Benjamin H Maskrey,Valerie B O&#x;Donnell
Benjamin H Maskrey
Eicosanoids are oxidized arachidonate-derived lipids products generated by lipoxygenase, cyclo-oxygenase and cytochrome P450. Their bioactivity is mediated via receptor-dependent mechanisms and they are involved in a diverse array of proces...
Mass spectrometry to detect the site specificity of advanced glycation/lipoxidation end-product formation on protein: some challenges and solutions [0.03%]
质谱在检测晚期糖基化/脂基化终产物修饰蛋白位点特异性上面临的挑战及解决方法
Jennifer M Ames
Jennifer M Ames
Formation of AGEs (advanced glycation end-products) and ALEs (advanced lipoxidation end-products) on proteins is associated with aging and various diseases of oxidative stress, notably diabetes and its complications. Modification of protein...