Min Bai,Qi Li,Yan-jiao Shao et al.
Min Bai et al.
The CRISPR/Cas9 system is a recently developed important technology for genome editing in cellular and animal models. Here we established a CRISPR/Cas9-based system of generating site-specific mutant mice using DNA double-strand breaks (DSB...
From gene editing to genome reconstitution: evolving techniques in yeast [0.03%]
从基因编辑到基因组重组：酵母技术的革新与发展
Chen Li,Hui-qiang Lou
Chen Li
Homologous recombination is one of the main repair pathways in response to DNA double strand break (DSB) in eukaryotes. Based on this, a series of techniques to introduce DSB have been developed in order to edit the DNA sequence of genome. ...
CRISPR/Cas9 genome editing technique and its application in site-directed genome modification of animals [0.03%]
CRISPR/Cas9基因编辑技术及其在动物定点基因组改造中的应用
Jin-wei Zhou,Qi-pin Xu,Jing Yao et al.
Jin-wei Zhou et al.
CRISPR/Cas system, which uses CRISPR RNAs (crRNAs) to guide Cas nuclease to silence invading nucleic acids, is self-defense system against exogenous virus or plasmid in bacteria and archaea. Through molecular modification, the typeⅡCRISPR/...
Wu Zheng,Feng Gu
Wu Zheng
The clustered regulatory interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) system mediates genome editing and is revolutionizing genetic researches. Scientists are able to manipulate the gene of interest from any organism with CRISPR/...
Jin-huan Li,Jia Shou,Qiang Wu
Jin-huan Li
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system from bacteria and archaea emerged recently as a new powerful technology of genome editing in virtually any organism. Due to it...
Use of genome editing tools in human stem cell-based disease modeling and precision medicine [0.03%]
基因组编辑工具在人类干细胞疾病模型和精准医学中的应用
Yu-da Wei,Shuang Li,Gai-gai Liu et al.
Yu-da Wei et al.
Precision medicine emerges as a new approach that takes into account individual variability. The successful conduct of precision medicine requires the use of precise disease models. Human pluripotent stem cells (hPSCs), as well as adult ste...
The molecular mechanism of CRISPR/Cas9 system and its application in gene therapy of human diseases [0.03%]
CRISPR/Cas9系统的分子机制及其在人类疾病基因治疗中的应用
Liang Qu,Hua-shan Li,Yun-han Jiang et al.
Liang Qu et al.
CRISPR/Cas system is an adaptive immune system that confers resistance to exogenous virus or plasmid in bacteria and archaea. In recent years, the booming CRISPR/Cas9 genome editing technology modified from type2 CRISPR/Cas adaptive immune ...
Qi-wei Shan,Cai-xia Gao
Qi-wei Shan
Genome editing technologies using engineered nucleases have been widely used in many model organisms. Genome editing with sequence-specific nuclease (SSN) creates DNA double-strand breaks (DSBs) in the genomic target sites that are primaril...
Exploration and implementation for the construction of the quaternary teaching system of medical genetics including teaching, practice, research and clinical application [0.03%]
医学遗传学教科练研四位一体教学体系的探索与实践
Feng-juan Zhou,Wen-mei Xie,Qiang Wang et al.
Feng-juan Zhou et al.
Medical genetics, the connection between basic and clinical medicine, is a subject with strong applicability and plays important role in modern medical education system. Based on years of teaching experience and during the construction of s...
The method of estimating bisulfite conversion rate in DNA methylation analysis [0.03%]
一种DNA甲基化分析亚硫酸氢盐转化率的估计方法
Yang-yang Liu,Heng-mi Cui
Yang-yang Liu
To establish an effective method to estimate the conversion rate of bisulfite-treated genomic DNA, TaqMan qPCR assay was performed using probes and primers that are specific for bisulfite-converted or -unconverted DNA standard samples separ...