[sgRNA design for the CRISPR/Cas9 system and evaluation of its off-target effects] [0.03%]
CRISPR/Cas9的sgRNA设计及脱靶效应评估
Sheng-song Xie,Yi Zhang,Li-sheng Zhang et al.
Sheng-song Xie et al.
The third generation of CRISPR/Cas9-mediated genome editing technology has been successfully applied to genome modification of various species including animals, plants and microorganisms. How to improve the efficiency of CRISPR/Cas9 genome...
Qi-wen Wang,Cui-fang Chang,Ning-ning Gu et al.
Qi-wen Wang et al.
Autophagy is a lysosome-mediated degradation pathway, which plays an important role in hepatic physiological and pathological processes, in eukaryotic cells. The liver has a remarkable regenerative capacity. After acute or chronic injury, t...
Li-li Yu,Wan-ru Dong,Ming-hui Chen et al.
Li-li Yu et al.
Gonadoblastoma (GB), a rare in situ germ cell tumor derived from sex cord and germ cells, is closely associated with gonadal dysgenesis. About 80% of GB individuals exhibit 46, XY female phenotype while the others are 45, XY and 46, XX with...
Wan-li Hu,Ai Gao
Wan-li Hu
With the rapid development of molecular biology technology, researchers have got much deeper understanding of the role of long non-coding RNA (lncRNA). It is not only indispensable for biological processes, but also plays an important role ...
Ting-ting Yin,Lu Wang,Guo-dong Wang
Ting-ting Yin
Transmissible tumors are a class of tumor that can be transmitted between individuals through living cells. So far, four types of transmissible tumors including canine transmissible venereal tumor (CTVT),Tasmanian devil facial tumor disease...
Development of a graphical user interface for sgRNAcas9 and its application [0.03%]
sgRNAcas9图形用户界面的开发及其应用
Chang-zhi Zhao,Yi Zhang,Guang-lei Li et al.
Chang-zhi Zhao et al.
The CRISPR/Cas9 genome editing technique is a powerful tool for researchers. However, off-target effects of the Cas9 nuclease activity is a recurrent concern of the CRISPR system. Thus, designing sgRNA (single guide RNA) with minimal off-ta...
Development and application of dual-fluorescence reporter systems for measuring specific nuclease activity based on SSA repair mechanism [0.03%]
基于SSA修复机制的特异性核酸酶活性检测系统的构建及应用研究
Fu-rong Han,Ling Wang,Kun Xu et al.
Fu-rong Han et al.
Reporter vector system has become an important method for measuring activity of specific nucleases because of its fast construction, simple modification, easy operation, economic effectiveness as well as its role in enriching positive cells...
Highly efficient CRISPR/Cas9-mediated targeted mutagenesis of multiple genes in Populus [0.03%]
高效简便的杨树多个基因CRISPR/Cas9敲除方法
Ting-ting Liu,Di Fan,Ling-yu Ran et al.
Ting-ting Liu et al.
The typeⅡCRISPR/Cas9 system (Clustered regularly interspaced short palindromic repeats /CRISPR-associated 9) has been widely used in bacteria, yeast, animals and plants as a targeted genome editing technique. In previous work, we have succ...
Generating amphioxus Hedgehog knockout mutants and phenotype analysis [0.03%]
刺尾鱼Hedgehog基因敲除突变体的制备及其表型分析
Hui Wang,Guang Li,Yi-quan Wang
Hui Wang
The amphioxus is a promising animal model for evolutionary-developmental studies due to its key position on the animal phylogenetic tree. In the present study, we reported a genetically modified amphioxus strain on the Hedgehog (Hh) gene lo...