Ferroptosis induction via genetic approaches - CRISPR/Cas9-based disruption on key anti-ferroptotic genes [0.03%]
基于CRISPR/Cas9技术的铁死亡诱导方法及关键抗铁死亡基因的破坏作用研究
Jiashuo Zheng,Weijia Zhang,Marcus Conrad
Jiashuo Zheng
Unlike apoptosis, necroptosis, or pyroptosis which are executed by dedicated proteins, ferroptosis is a distinct form of regulated cell death driven by lipid peroxidation downstream of metabolic dysfunction. In most physiological settings, ...
Jie Lu,Haohao Lu,Sudan He
Jie Lu
Necroptosis is a regulated form of cell death that relies on receptor-interacting serine/threonine-protein kinase 3 (RIPK3) -mediated phosphorylation of mixed-lineage kinase domain-like pseudokinase (MLKL). This phosphorylation drives MLKL ...
Luying Zheng,Daichao Xu
Luying Zheng
In vitro cultured organoids exhibit highly similar structures to their corresponding tissues and organs. Intestinal organoids derived from intestinal stem cells faithfully recapitulate the key characteristics of native intestinal epithelium...
Assessing the activity of caspase-activated pannexin-1 channels by flow cytometry [0.03%]
流式细胞术评估caspase激活的pannexin-1通道的功能活动
Michael B P F Hector,Ivan K H Poon,Jascinta P Santavanond
Michael B P F Hector
Pannexin 1 (PANX1) is a ubiquitously expressed plasma membrane channel that plays an important role in maintaining cellular homeostasis through the release of small signalling metabolites. Under cell death settings, PANX1 channels can becom...
Sensitive detection of lysosomal membrane permeabilization using the galectin puncta assay [0.03%]
利用半乳糖凝集素点状检测技术敏感检测溶酶体膜渗透性
Konstantinos Danias,Victoria Alexandra Bjerregaard,Marja Jäättelä
Konstantinos Danias
The Lysosomal Galectin Puncta Assay is a microscopy-based technique able to detect even minor lysosomal leakage with high sensitivity. This protocol describes the detection of galectin puncta as markers of lysosomal membrane permeabilizatio...
Weimin Sun,Daichao Xu
Weimin Sun
High-throughput screening (HTS) has become an essential tool for identifying potential inducers and inhibitors of programmed cell death (PCD), a process crucial for maintaining cellular homeostasis and regulating various physiological and p...
Flora Doffe,Marion Leduc,Xiaolian Deng et al.
Flora Doffe et al.
High-throughput screening (HTS) plays an essential role in oncological drug discovery by facilitating the identification of novel anticancer agents through the evaluation of cytotoxic responses in malignant cells. Advances in automation, im...
The heat-ramp method to study regulated cell death in a pathogenic yeast Cryptococcus neoformans [0.03%]
利用热激法研究致病酵母新型隐球菌中的程序性细胞死亡现象
Madhura Kulkarni,Yining Liu,Quanxi Cheng et al.
Madhura Kulkarni et al.
Human fungal pathogens cause a significant public health burden. While no reliable surveilence data are available, estimations suggest that 1 billion infections and over 2 million deaths are attributable to fungal infections annually worldw...
3D bioluminescence imaging assays for measuring in vitro CAR T cell cytotoxicity [0.03%]
三维生物发光成像测定在体外CAR-T细胞细胞毒性测量中的应用
Eugenia Cambiaso,Benjamin Motais,Ralph Geoffrey Tieland et al.
Eugenia Cambiaso et al.
For the past decades, many advances were made in the field of immunotherapies, most notably the development of chimeric antigen receptor (CAR) T cells, that allowed development of potent and targeted treatment options against cancers and ot...
Reprogramming T cell fate through antibody-mediated galectin-1 blockade [0.03%]
通过抗体介导的半乳糖凝集素-1阻断重新编程T细胞命运
Roxana Kiyomi Mizutamari,Juan M Pérez Sáez,Alejandro J Cagnoni et al.
Roxana Kiyomi Mizutamari et al.
Galectins, a family of highly conserved β-galactoside-binding proteins, favor tumor progression by modulating multiple hallmarks of cancer. Galectin-1 (GAL1), a prototype member of this family, plays essential roles in both tumor immunosup...