Fundamental significance of DNA mass-sequencing factory for biological sciences in future [0.03%]
DNA测序工厂对未来的生命科学具有重要意义
A Wada
A Wada
We are now entering an era of the mass-analysis of genetic information, which will signal the beginning of the study of living organisms on the basis of their most detailed plan: the DNA base-sequence. The researcher's essential requirement...
S Sugai,M Ikeguchi
S Sugai
Comparisons of the conformation behavior between LA and LZ (especially BLA and HEL) are described, which have contributed to the development and understanding of the molten globule folding intermediate of protein. BLA (and other LAs) is cle...
Thermodynamics of protein folding: effects of hydration and electrostatic interactions [0.03%]
蛋白质折叠的热力学：溶剂化和静电相互作用的影响
T Ooi
T Ooi
DNA base sequences contain a variety of information, and this information flows from DNA to a protein through the steps of transcription and translation. The one dimensional information given from DNA is the amino acid sequence of the prote...
Structure of RecA-DNA complex and mechanism of DNA strand exchange reaction in homologous recombination [0.03%]
同源重组中RecA-DNA复合物的结构及DNA交换反应机制
M Takahashi,B Nordén
M Takahashi
The importance of filament formation of RecA for the DNA strand exchange reaction, both in vivo and in vitro, is established. RecA forms a very long and relatively stiff filament by binding around DNA with high cooperativity. The monomer un...
A species-specific interaction of rad51 and rad52 proteins in eukaryotes [0.03%]
真核生物中rad51和rad52蛋白的种属特异性互作关系
T Ogawa,A Shinohara,T Ikeya
T Ogawa
The structures and properties of the Rad51 and Rad52 proteins in eukaryotes are described. Both proteins form a complex and are responsible for recombination and repair reactions. The N-terminal region of the Rad51 protein interacts with th...
Multi-site-specific endonucleases and the initiation of homologous genetic recombination in yeast [0.03%]
多宿主特异性内切核酸酶与酵母同源遗传重组的启动
T Shibata,K Nakagawa,N Morishima
T Shibata
The notion that homologous recombination is a regulated biological process is not a familiar one. In yeasts, homologous recombination and most site-specific ones are initiated by site-specific double-stranded breaks that are introduced with...
H Ogawa,K Johzuka,T Nakagawa et al.
H Ogawa et al.
Mutants defective in meiotic recombination were isolated using a disomic haploid strain of S. cerevisiae, and were classified into 11 genes. Two, MRE2 and MRE11, are new genes and nine are previously identified genes. The mre2 and mre11 del...
H Shinagawa,H Iwasaki
H Shinagawa
Recent genetic and biochemical studies revealed the mechanisms of late stage of homologous recombination in E. coli. A central intermediate of recombination called "Holliday structure", in which two homologous duplex DNA molecules are linke...
T Yonesaki
T Yonesaki
The substantial process of general DNA recombination consists of production of ssDNA, exchange of the ssDNA and its homologous strand in a duplex, and cleavage of branched DNA to maturate recombination intermediates. Ten genes of T4 phage a...
K Morimatsu,T Horii
K Morimatsu
To investigate the DNA binding site of RecA protein, we constructed 15 recA mutants having alterations in the regions homologous to the other ssDNA binding proteins. The in vivo analyses showed that the mutational change at Arg243, Lys248, ...