Electron crystallography of macromolecular periodic arrays on phospholipid monolayers [0.03%]
磷脂单层上大分子周期性阵列的电子晶体学研究
W Chiu,A J Avila-Sakar,M F Schmid
W Chiu
Electron crystallography has the potential of yielding structural information equivalent to x-ray diffraction. The major difficulty has been preparing specimens with the required structural order and size for diffraction and imaging in the ...
Assembly of protein structures on liposomes by non-specific and specific interactions [0.03%]
利用非特异性和特异性相互作用在脂质体上组装蛋白质结构
O D Velev
O D Velev
We investigate different schemes for fabrication of nanometer sized assemblies that consist of a liposome core over which a shell of ferritin is attached. Three distinct interactions were used for this assembly: (i) Electrostatic attraction...
K Fujita,S Kimura,Y Imanishi et al.
K Fujita et al.
We investigated supramolecular assemblies of various hydrophobic helical peptides. The assemblies were formed at the air/water interface or in aqueous medium. The hexadecapeptide, Boc-(Ala-Aib)s-OMe (BA16M), was reported to take alpha-helic...
Molecular handling of photosynthetic proteins for molecular assembly construction [0.03%]
用于分子装配构建的光合蛋白质的分子操作技术
J Miyake,M Hara
J Miyake
Methods of constructing proteins were examined with special reference to the molecular assembly using photosynthetic RCs as membrane proteins. Molecular assemblies at the interfaces were studied by LB films, adsorption to the surface and re...
H Yoshimura
H Yoshimura
A simple 2D crystallization method using unfolded protein film as a supporting film of crystals was described, which allows modification of protein surfaces by injecting chemical reagents into the subphase after the crystal formation. As an...
E Adachi,K Nagayama
E Adachi
We were successful in developing a technique to form protein array developed directly on solid surfaces. This promising array formation revealed two new concepts of the ordering mechanism: the lifetime of a secondary minimum and secondary f...
U B Sleytr,D Pum,M Sára
U B Sleytr
Two-dimensional crystalline bacterial S-layers composed of identical protein or glycoprotein subunits turned out to be ideal materials for the development of biomimetic membranes and new approaches in molecular nanotechnology. These isoporo...
Criticality found in a model for orientational ordering of protein arrays [0.03%]
蛋白质阵列取向有序化的模型中的临界性研究
M Hogyoku
M Hogyoku
MC-PSRG analysis allowed us to reduce criticalities A, B, and C found in the poker chip model, respectively, to ones of the Ising universality, of the 3-state Potts universality, and of the KT-like phase. We note that not only the KT-like p...
Significant role of electrostatic interactions for stabilization of protein assemblies [0.03%]
静电作用在蛋白质组装体稳定中的重要作用
T Takahashi
T Takahashi
Contribution of electrostatic interactions to stability of BPTI orthorhombic, pig-insulin cubic crystals, and horse L ferritin crystals was evaluated with numerical calculation of Poisson-Boltzmann equation based on a dielectric model. The ...
P A Kralchevsky
P A Kralchevsky
To investigate the mechanism of formation of 2D arrays of protein macromolecules in liquid films we carried out model experiments with micron-sized latex particles. The direct observations revealed that the process of ordering is triggered ...