IS1-encoded proteins, InsA and the InsA-B'-InsB transframe protein (transposase): functions deduced from their DNA-binding ability [0.03%]
IS1编码蛋白InsA以及结合DNA能力所推断的其转座酶功能InsA-B'-InsB.frameshift蛋白（转座酶）
N Sekino,Y Sekine,E Ohtsubo
N Sekino
Insertion sequence IS1 encodes a transframe protein, InsA-B'-InsB, which is produced from two out-of-phase reading frames, insA and B'-insB, by translational frameshifting at a run of adenines. Unless the frameshifting event occurs, the Ins...
A novel assay for illegitimate recombination in Escherichia coli: stimulation of lambda bio transducing phage formation by ultra-violet light and its independence from RecA function [0.03%]
大肠杆菌错误型接合作实验的新方法：紫外线对λ-bio噬菌体形成的诱导作用及其与RecA功能的无关性
H Ikeda,H Shimizu,T Ukita et al.
H Ikeda et al.
We developed a novel assay system for illegitimate recombination, in which the frequency of the formation of lambda Spi- phages formed during prophage induction was measured with an E. coli P2 lysogen as the indicator bacteria. Since almost...
T Komano,S R Kim,T Yoshida
T Komano
Gene organization of the 54-kb transfer region of IncI1 plasmid R64 was deduced from the DNA sequence. Forty-eight ORFs were found in this region. A unique DNA rearrangement designated shufflon is located at the downstream region of an oper...
C Oka,M Kawaichi
C Oka
We have constructed transgenic mice carrying an artificial substrate of V(D)J recombination. In this substrate, the only DNA fragments derived from Ig genes were short stretches of recombination signal sequences. This artificial substrate w...
A differential cloning procedure for rearranged or altered genomic DNA based on in-gel competitive reassociation [0.03%]
一种基于凝胶中竞争性复性的差异克隆程序，用于重组或改变的基因组DNA
R Kiyama,S Inoue,R Ohki et al.
R Kiyama et al.
We have developed a substantially improved differential cloning procedure designed for cloning anonymous altered restriction DNA fragments from higher organisms. The improvements include (i) in-gel dissociation and reassociation of biotinyl...
A new type of E. coli recombinational hotspot which requires for activity both DNA replication termination events and the Chi sequence [0.03%]
一种新的大肠杆菌重组热点类型，这种类型的热点活性需要终止事件和Chi序列共同作用
T Horiuchi,H Nishitani,T Kobayashi
T Horiuchi
In E. coli rnh- mutants we identified chromosome-derived, specific DNA fragments termed Hot DNA. When the DNA in the ccc form is integrated into the E. coli genome by homologous recombination to form a directly repeated structure, a strikin...
T Shiroishi,T Koide,M Yoshino et al.
T Shiroishi et al.
The molecular mapping of recombinational breakpoints in the proximal region of the mouse MHC has revealed four hotspots at which breakpoints are clustered. A direct comparison of the nucleotide sequences of two independent hotspots revealed...
Y Hotta,K Furukawa,S Tabata
Y Hotta
We have discussed and/or demonstrated the following: 1. Many enzymes and structural proteins have been identified as meiosis-specific proteins. These can be classified according to their metabolic behavior. 2. We obtained and analyzed 18 cD...
T Suzuki,H Inomata
T Suzuki
The ionic mechanism of excitation in intestinal smooth muscles has been reviewed. Mammalian smooth muscles are a very important group of excitable cells. There exists a considerable number of reports on the electrical and mechanical propert...
H Chino,R G Downer
H Chino
Lipophorin, formerly called the "diacylglycerol-carrying lipoprotein," exists in the hemolymph of many insects including locust, cockroach, and silkworm. A rapid and efficient method has been developed for the purification of lipophorin, wh...
Comparative Study
Advances in biophysics. 1982:15:67-92. DOI:10.1016/0065-227x(82)90005-3 1982