Characterization of viroid-like RNAs associated with the citrus exocortis syndrome [0.03%]
与柑橘外皮症相关的类病毒状RNA的表征
N Duran-Vila,R Flores,J S Semancik
N Duran-Vila
Three additional viroid-like RNA species (RNA-I, -II, and -III), smaller than the 371-nucleotide citrus exocortis viroid (CEV), have been identified in citron (Citrus medica) trees which display symptoms of the exocortis disease. The three ...
Influence of exogenous viral coat protein on the cotranslational disassembly of tobacco mosaic virus (TMV) particles in vitro [0.03%]
外源病毒衣壳蛋白对烟草花叶病毒(TMV)颗粒体外共翻译拆卸的影响
T Michael,A Wilson,P A Watkins
T Michael
Free coat protein subunits (at 0.1-1.0 mg/ml) from tobacco mosaic virus (TMV) preferentially inhibit the cotranslational disassembly of pH 8-washed virions in vitro. The inhibitory effect is greater than in incubations programmed with naked...
Construction of a recombinant vaccinia virus which expresses immunoreactive plant virus proteins [0.03%]
构建表达免疫反应性植物病毒蛋白的重组痘病毒
W G Dougherty,C A Franke,D E Hruby
W G Dougherty
A chimeric transcription unit was assembled consisting of a vaccinia virus promoter linked to a 2400 bp(3) double-stranded complementary DNA (cDNA) made to the 3' end of the genomic RNA of the plant pathogen, tobacco etch virus (TEV). Marke...
In vitro viral RNA synthesis by a subcellular fraction of TMV-inoculated tobacco protoplasts [0.03%]
由TMV感染的烟草原生质体制备的亚细胞组分体外合成病毒RNA
Y Watanabe,Y Okada
Y Watanabe
A subcellular fraction which can synthesize viral RNA and subgenomic RNA in vitro was prepared from tobacco mosaic virus (TMV)-inoculated tobacco protoplasts. S(1)-Resistant fragment analysis with strand specific TMV cDNA showed that a larg...
Isolation of velvet tobacco mottle virus capable of replication with and without a viroid-like RNA [0.03%]
一种能够在有或无类似维管束病菌的RNA情况下复制的美丽 Tobacco Mottle 病毒的分离
R I Francki,C J Grivell,K S Gibb
R I Francki
Field isolates of velvet tobacco mottle virus (VTMoV) induce severe symptoms in Nicotiana clevelandii and encapsidate viroid-like RNA reported to be essential for virus infection. An isolate of the virus producing only mild symptoms on N. c...
Evidence that tobacco mosaic virus particles disassemble contranslationally in vivo [0.03%]
烟草花叶病毒在活细胞中与翻译同步解体的证据
J G Shaw,K A Plaskitt,T M Wilson
J G Shaw
The possibility that a cotranslational disassembly mechanism, similar to that observed when pH 8-treated tobacco mosaic virus (TMV) particles are incubated in an in vitro translation system [T. M. A. Wilson. (1984), Virology 137, 255-265], ...
Alternate pathway of entry of budded Autographa californica nuclear polyhedrosis virus: fusion at the plasma membrane [0.03%]
融合于细胞膜上的草地贪夜蛾核型多角体病毒芽孢入侵的替代途径
L E Volkman,P A Goldsmith,R T Hess
L E Volkman
Recently we reported that the budded phenotype of Autographa californica nuclear polyhedrosis virus (AeNPV BV) functionally entered IPLB-SF-21 cells by adsorptive endocytosis and that neutralizing monoclonal antibody AcV1 acted by preventin...
The kinetics of swelling of southern bean mosaic virus: a study using photon correlation spectroscopy [0.03%]
南方菜豆花叶病毒膨胀动力学的光子相关谱研究
M Brisco,C Haniff,R Hull et al.
M Brisco et al.
Southern bean mosaic virus swells upon removal of Ca2+ at pH 8.25. Virions do not seem to aggregate significantly; the z-average hydrodynamic diameter increases from 29.9 nm to 44.0 nm. Swelling is virtually complete within 3 min, and swoll...
Swelling of isometric and of bacilliform plant virus nucleocapsids is required for virus-specific protein synthesis in vitro [0.03%]
等径及杆状植物病毒核衣壳的膨胀是体外合成病毒特异蛋白的必需条件
M Brisco,R Hull,T M Wilson
M Brisco
Encapsidated RNA of cowpea chlorotic mottle virus (CCMV), brome mosaic virus (BMV), alfalfa mosaic virus, and southern bean mosaic virus can act as templates for protein synthesis when preswollen virions are added to a wheat germ extract, o...
Artifacts are responsible for the translational activity of polyribosome preparations isolated from alfalfa mosaic virus-infected tobacco leaves [0.03%]
Alfalfa mosaic病毒感染的烟草叶片中分离的多聚核糖体复合物的翻译活性由一种亚细胞结构决定
L Neeleman,P Lindhout,L van Vloten-Doting
L Neeleman
We observed that polyribosome preparations isolated from alfalfa mosaic virus (A1MV)-infected tobacco leaves were contaminated with virion-derived material which could not be removed completely by sucrose gradient centrifugation or by magne...