Photobleaching Analysis of Fluorescent Proteins in Two-Photon Microscopy at High Repetition Rates [0.03%]
高重复率双光子显微镜中荧光蛋白的光漂白分析
Chun Hung Weng,Kyu Young Han
Chun Hung Weng
Severe photobleaching in two-photon excitation fluorescence microscopy (TPM) has limited its potential for long-term and quantitative imaging of live cells and tissues. One solution is to excite fluorophores with a high-repetition rate whic...
Combining deep learning approaches and point spread function engineering for simultaneous 3D position and 3D orientation measurements of fluorescent single molecules [0.03%]
结合深度学习方法和点扩散函数工程技术实现荧光单分子的三维位置和取向同时测量
Pierre Jouchet,Anish R Roy,W E Moerner
Pierre Jouchet
Point Spread Function (PSF) engineering is an effective method to increase the sensitivity of single-molecule fluorescence images to specific parameters. Classical phase mask optimization approaches have enabled the creation of new PSFs tha...
Instantaneous non-diffracting light-sheet generation by controlling spatial coherence [0.03%]
通过控制空间相干性实现光片的瞬时非衍射性生成
Jialei Tang,Kyu Young Han
Jialei Tang
We demonstrate single-shot non-diffracting light-sheet generation by controlling the spatial coherence of light. A one-dimensional coherent beam, created by either increasing the spatial coherence of an LED or decreasing the spatial coheren...
Ting Chean Khoo,Anna Sharikova,Alexander Khmaladze
Ting Chean Khoo
We present a dual wavelength digital holographic technique for three-dimensional microscopic imaging of layered structures, where layers are separated from one another by the axial distances exceeding the wavelength of imaging light. Our me...
Measurement of micro-harmonic vibration from optical feedback interferometry using wavelet trend analysis [0.03%]
使用小波趋势分析从光学反馈干涉仪中测量微谐振动
Vibhor Kumar Bhardwaj,Surita Maini
Vibhor Kumar Bhardwaj
Self-mixed optical feedback interferometry based laser sensors show promising results in the measurement of the vibration frequency. To date several measurement methods have been developed to extract the vibration information from the self-...
High-efficiency non-diffractive generator of arbitrary vectorial optical fields with minimal optical elements [0.03%]
高效低元非衍射任意矢量光场发生器
Billy Lam,Chunlei Guo
Billy Lam
Tailoring electromagnetic waves has a wide range of applications, such as optical trapping, focus engineering, imaging, laser cutting, and optical communication. To do so, the spatial distribution of at least one of the four degrees of free...
Design of 45° periscopic visual field expansion device for peripheral field loss [0.03%]
用于视野缺损的45°潜望式视场扩展装置设计
Hee-Jin Choi,Eli Peli,Minyoung Park et al.
Hee-Jin Choi et al.
Patients with visual field loss have difficulty in mobility due to collision with pedestrians/obstacles from the blind side. In order to retrieve the lost visual field, prisms which deflect the field from the blind to the seeing side, have ...
Switchable detector array scheme to reduce the effect of single-photon detector's deadtime in a multi-bit/photon quantum link [0.03%]
一种可切换检测器阵列方案减少多进制/多光子量子通信链路中单光子探测器死时间影响
Cong Liu,Yongxiong Ren,Jiapeng Zhao et al.
Cong Liu et al.
We explore the use of a switchable single-photon detector (SPD) array scheme to reduce the effect of a detector's deadtime for a multi-bit/photon quantum link. The case of data encoding using M possible orbital-angular-momentum (OAM) states...
Spatial and spectral imaging of point-spread functions using a spatial light modulator [0.03%]
基于空间光调制器的点扩散函数的空间和谱学成像
Sravan Munagavalasa,Bryce Schroeder,Xuanwen Hua et al.
Sravan Munagavalasa et al.
We develop a point-spread function (PSF) engineering approach to imaging the spatial and spectral information of molecular emissions using a spatial light modulator (SLM). We show that a dispersive grating pattern imposed upon the emission ...
Patterned Synthesis of ZnO Nanorod Arrays for Nanoplasmonic Waveguide Applications [0.03%]
用于纳米等离激元波导应用的ZnO纳米线阵列的合成图案研究
Thomas L Lamson,Sahar Khan,Zhifei Wang et al.
Thomas L Lamson et al.
We report the patterned synthesis of ZnO nanorod arrays of diameters between 50 nm and 130 nm and various spacings. This was achieved by patterning hole arrays in a polymethyl methacrylate layer with electron beam lithography, followed by c...