[Influence of Homology Arm Length and Structure on the Efficiency of Long Transgene Integration into a Cleavage Site Induced by SpCas9 or AsCpf1] [0.03%]
【同源臂长度和结构对SpCas9或AsCpf1介导的基因组打靶位点整合长外源片段效率的影响】
J A Taran,R R Mintaev,D V Glazkova et al.
J A Taran et al.
One of the promising new approaches to the treatment of HIV infection is CRISPR/Cas-mediated knockout of the CCR5 receptor gene followed by the integration of an anti-HIV gene into the break site. Numerous studies have focused on the knocko...
[Dynamics of Transgene Expression in the MVA Vaccine Vector under the Control of the p11, p13.5, pLEO160, p7.5, and mH5 Promoters and Independence of the Transgene Expression Level from the Insertion Locus] [0.03%]
[MVA疫苗载体中受p11、p13.5、pLEO160、p7.5和mH5启动子调控的报告基因的表达动力学及其与插入位点无关性研究]
O V Orlova,D V Glazkova,O N Sidorova et al.
O V Orlova et al.
The modified vaccinia virus Ankara (MVA), characterized by high immunogenicity and proven safety, is considered a promising vector for vaccine development. An undeniable advantage of the MVA vector is its high capacity and the ability to in...
[Improving the Efficiency and Safety of Human CCR5 Gene Editing by Selection of Optimal Guide RNAs for SpCAS9 and CAS12A] [0.03%]
选择sgRNA提高SpCas9和Cas12a编辑人CCR5的效率与安全性
R R Mintaev,D V Glazkova,J A Taran et al.
R R Mintaev et al.
Advances in CRISPR/Cas-mediated genome editing have opened up treatment alternatives for many human diseases, including HIV infection. Knockout of the CCR5 gene as a potential way to treat HIV infection has long been studied. Here we analyz...
[Placental Tissue Transcriptomics as a Tool to Identify the Molecular Mechanisms of Great Obstetrical Syndromes] [0.03%]
胎盘组织转录组学在揭示重大妊娠综合征分子机制中的应用研究
E A Trifonova,A V Markov,A A Zarubin et al.
E A Trifonova et al.
Numerous histological studies have demonstrated that impaired placentation processes are involved in the key pathogenetic mechanisms of great obstetrical syndromes (GOSs). However, the molecular basis of this discovery is still unclear. The...
[Identification of Genetic Markers of Predisposition to Thrombogenic Diseases by Minisequencing Analysis: Reagent Set "SNP2-TMG"] [0.03%]
[用于检测血栓性疾病易感性基因标记的Minisequencing分析试剂盒“SNP2-TMG”的鉴定]
A V Grudo,I V Haidukevich,G V Sergeev
A V Grudo
Thrombogenic risk factors (blood coagulation disorders and thrombophilia) are the cause of cardiovascular diseases, among which genetic factors are worth highlighting (genetic polymorphism of the blood coagulation system, angiogenesis facto...
[Transcription Factor PAX4: Role in Differentiation of Insulin- Producing β Cells during Pancreas Development and Association with Diabetes] [0.03%]
转录因子PAX4在胰腺发育中胰岛β细胞分化的作用及其与糖尿病的关系
A I Melnikova,T S Krasnova,P M Rubtsov
A I Melnikova
PAX4 (Paired Box 4) is a transcription factor that is expressed mainly in the pancreas and plays a key role in the development of insulin-producing β cells at the embryonic stage. In mature cells, PAX4 acts as a main regulator of their ada...
[Prognostic Potential of hsa-miR-16-5p, hsa-miR-125b-5p, and hsa-miR-181a-5p for the Formation of Groups of Increased Risk of Breast Cancer under Radiation Exposure] [0.03%]
[hsa-miR-16-5p、hsa-miR-125b-5p和hsa-miR-181a-5p在辐射暴露增加乳腺癌发病风险人群分组中的预后潜能]
M A Yanishevskaya,E A Blinova,A V Akleyev
M A Yanishevskaya
Breast cancer (BC) is a multifactorial disease that is characterized by various genetic and epigenetic changes that occur due to the effect of various factors including that of environmental etiological agents. The obtained scientific data ...
[Development of Multiplex Real-Time RT-PCR to Determine the Expression Levels of Toll-Like Receptor Genes] [0.03%]
toll-like受体基因表达量检测的多重实时RT-PCR技术建立与发展
S A Salamaikina,V I Korchagin,K O Mironov
S A Salamaikina
Immune response gene expression analysis is an important task in studies of interactions between a host and an infectious agent. Many approaches to this task have been developed, but despite significant progress, the problem of selecting a ...
[Transcriptomics and the "Curse of Dimensionality": Monte Carlo Simulations of ML-Models as a Tool for Analyzing Multidimensional Data in Tasks of Searching Markers of Biological Processes] [0.03%]
transcriptomics与“维度诅咒”：作为寻找生物过程标记的多维数据分析工具的极大似然模型的蒙特卡洛模拟
G J Osmak,M V Pisklova
G J Osmak
High-throughput transcriptomic research methods provide the assessment of a vast number of factors valuable for researchers. At the same time, "curse of dimensionality" issues arise, which lead to increasing the requirements on data process...
[Mechanism of Thiocyanate Dehydrogenase Functioning Based on Structural Data] [0.03%]
基于结构数据的异硫氰酸酯脱氢酶作用机制研究
K M Polyakov,S Gavryushov
K M Polyakov
Thiocyanate dehydrogenase is enzyme catalyzing transformation of a thiocyanate ion into a cyanate ion with outcome of two electrons, two protons and a neutral atom of sulphur. Earlier structures of thiocyanate dehydrogenase from Thioalkaliv...