A V Shuvalov,A A Klishin,N S Biziaev et al.
A V Shuvalov et al.
Eukaryotic translation release factor eRF1 is an important cellular protein that plays a key role in translation termination, nonsense-mediated mRNA decay (NMD), and readthrough of stop codons. The amount of eRF1 in the cell influences all ...
[The Drosophila Zinc Finger Proteins Aef1 and CG10543 Are Co-Localized with SAGA, SWI/SNF, and ORC Complexes on Gene Promoters and Involved in Transcription Regulation] [0.03%]
果蝇锌指蛋白Aef1和CG10543与SAGA、SWI/SNF和ORC复合物共同定位在基因启动子上并参与转录调控
J V Nikolenko,M M Kurshakova,D V Kopytova et al.
J V Nikolenko et al.
In previous studies, we purified the DUB-module of the Drosophila SAGA complex and showed that a number of zinc proteins interact with it, including Aef1 and CG10543. In this work, we conducted a genome-wide study of the Aef1 and CG10543 pr...
[The Drosophila Zinc Finger Protein CG9609 Interacts with the Deubiquitinating (DUB) Module of the SAGA Complex and Participates in the Regulation of Transcription] [0.03%]
[Strausser激酶复合物参与果蝇RNA聚合酶II转录的激活]
J V Nikolenko,M M Kurshakova,D V Kopytova et al.
J V Nikolenko et al.
In previous studies, we found that the zinc finger proteins Su(Hw) and CG9890 interact with the Drosophila SAGA complex and participate in the formation of the active chromatin structure and transcription regulation. In this research, we di...
[Sensitivity of Primary Human Glioblastoma Cell Lines to the Mumps Virus Vaccine Strain] [0.03%]
人源胶质母细胞瘤原代细胞系对腮腺炎病毒减毒活疫苗株的敏感性研究
E Yu Nikolaeva,Y R Zhelayeva,O Yu Susova et al.
E Yu Nikolaeva et al.
The sensitivity of human glioblastoma cells to virus-mediated oncolysis was investigated on five patient-derived cell lines. Primary glioblastoma cells (Gbl13n, Gbl16n, Gbl17n, Gbl25n, and Gbl27n) were infected with tenfold serial dilutions...
[Donor DNA Modification with Cas9 Targeting Sites Improves the Efficiency of MTC34 Knock-in into the CXCR4 Locus] [0.03%]
Cas9靶点修饰供体DNA可提高MTC34定点整合入CXCR4座位的效率
M V Shepelev,D S Komkov,D S Golubev et al.
M V Shepelev et al.
To successfully apply the genome editing technology using the CRISPR/Cas9 system in the clinic, it is necessary to achieve a high efficiency of knock-in, which is insertion of a genetic construct into a given locus of the target cell genome...
[Methods to Increase the Efficiency of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line] [0.03%]
[HIV-1融合抑制肽MT-C34编码构建体敲入CEM/R5T细胞系CXCR4位点效率提高的方法]
D S Golubev,D S Komkov,M V Shepelev et al.
D S Golubev et al.
The low knock-in efficiency, especially in primary human cells, limits the use of the genome editing technology for therapeutic purposes, rendering it important to develop approaches for increasing the knock-in levels. In this work, the eff...
[Amino Acid Substitution Patterns in the E6 and E7 Proteins of HPV Type 16: Phylogeography and Evolution] [0.03%]
[HPV16型E6和E7蛋白的氨基酸置换规律：谱系地理学与进化]
E E Zelenova,A A Karlsen,D V Avdoshina et al.
E E Zelenova et al.
The E6 and E7 proteins of the high risk human papillomaviruses (HR HPVs) play a key role in the oncogenesis associated with papillomavirus infection. Data on the variability of these proteins are limited, and the factors affecting their var...
[How to Shift the Equilibrium of DNA Break Repair in Favor of Homologous Recombination] [0.03%]
如何使DNA修复向同源重组倾斜
O A Averina,S A Kuznetsova,O A Permyakov et al.
O A Averina et al.
The CRISPR/Cas technology of targeted genome editing made it possible to carry out genetic engineering manipulations with eukaryotic genomes with a high efficiency. Targeted induction of site-specific DNA breaks is one of the key stages of ...
O A Averina,S A Kuznetsova,O A Permyakov et al.
O A Averina et al.
Modern genetic engineering technologies, such as base editing and prime editing (PE), have proven to provide the efficient and reliable genome editing tools that obviate the need for donor templates and double-strand breaks (DSBs) introduce...
[Revision of Functionally Relevant and Widely Expressed Long Non-Coding RNAs] [0.03%]
[功能相关的广泛表达的长非编码RNA的修订]
D Konina,M Skoblov
D Konina
Long non-coding RNAs (lncRNAs) are involved in many cellular processes while displaying high tissue specificity. In contrast, protein-coding genes, including the category of housekeeping ones, exhibit broad expression patterns. The aim of t...