Ebola hemorrhagic fever: Properties of the pathogen and development of vaccines and chemotherapeutic agents [0.03%]
埃博拉出血热：病原特性与疫苗和化疗药物的开发
O I Kiselev,A V Vasin,M P Shevyryova et al.
O I Kiselev et al.
Ebola hemorrhagic fever (EHF) epidemic currently ongoing in West Africa is not the first among numerous epidemics in the continent. Yet it seems to be the worst EHF epidemic outbreak caused by Ebola virus Zaire since 1976 as regards its ext...
O N Ilinskaya,R Shah Mahmud
O N Ilinskaya
Many ribonucleases (RNases) are able to inhibit the reproduction of viruses in infected cell cultures and laboratory animals, but the molecular mechanisms of their antiviral activity remain unclear. The review discusses the well-known RNase...
Baculovirus expression systems for production of recombinant proteins in insect and mammalian cells [0.03%]
昆虫和哺乳动物细胞中杆状病毒表达系统用于重组蛋白的生产
S N Beljelarskaya
S N Beljelarskaya
Baculovirus vector systems are extensively used for the expression of foreign gene products in insect and mammalian cells. New advances increase the possibilities and applications of the baculovirus expression system, which makes it possibl...
Polymorphism of 2'-5'-oligoadenylate synthetase (OAS) genes, associated with predisposition to severe forms of tick-borne encephalitis, in human populations of North Eurasia [0.03%]
北欧亚人种中与严重蜱传脑炎易感性相关的2'-5'-寡腺苷酸合成酶(OAS)基因多态性研究
A V Barkhash,V N Babenko,V F Kobzev et al.
A V Barkhash et al.
2'-5'-oligoadenylate synthetases are a family of interferon-induced enzymes playing an important role in antiviral defense in mammals. In the human genome, three genes encoding functional synthetases (OAS1, OAS2 and OAS3) form a cluster. Pr...
S P Chumakov,V S Prassolov
S P Chumakov
Separation of DNA replication and transcription, which occur in the nucleus, from protein synthesis, which occurs in the cytoplasm, allows a more precise regulation of these processes. Selective exchange of macromolecules between the two co...
Screening efficient siRNAs in vitro as the candidate genes for chicken anti-avian influenza virus H5N1 breeding [0.03%]
高效抗禽流感病毒H5N1.siRNA筛选及家鸡品系创制研究
P Zhang,J G Wang,J Y Wan et al.
P Zhang et al.
The frequent disease outbreaks caused by avian influenza virus (AIV) not only affect the poultry industry but also pose a threat to human safety. To address the problem, RNA interference (RNAi) has recently been widely used as a potential a...
Synthesis in Escherichia coli cells and characterization of the active exoribonuclease of severe acute respiratory syndrome coronavirus [0.03%]
肺炎萨斯冠状病毒活性外切体RNA酶的表达及鉴定
P Chen,T Hu,M Jiang et al.
P Chen et al.
The nsp14 protein, an exoribonuclease of the DEDD superfamily encoded by severe acute respiratory syndrome coronavirus (SARS-CoV), was expressed in fusion with different affinity tags. The recombinant nsp14 proteins with either GST fusion o...
Use of the recombinant nonstructural 3A, 3B, and 3AB proteins of foot-and-mouth disease virus in indirect ELISA for differentiation of vaccinated and infected cattle [0.03%]
利用口蹄疫病毒重组非结构蛋白3A、3B和3AB进行免疫层析竞争法鉴别牛的疫苗免疫与野毒感染
A S Yakovleva,A V Shcherbakov,A V Kan&#x;shina et al.
A S Yakovleva et al.
Recombinant foot-and-mouth disease virus (FMDV) proteins 3A, 3B, and 3AB were produced by expressing the corresponding genes in Escherichia coli and purified by metal-chelate affinity chromatography. The recombinant proteins were used as an...
A P Gmyl,V I Agol
A P Gmyl
Recombination is widespread among RNA viruses, but many molecular mechanisms of this phenomenon are still poorly understood. It was believed until recently that the only possible mechanism of RNA recombination is replicative template switch...
V I Agol
V I Agol
Picornaviruses are small animal viruses with positive-strand genomic RNA, which is translated using cap-independent internal translation initiation. The key role in this is played by ciselements of the 5"-untranslated region (5"-UTR) and, i...