Genetic Engineering Systems to Study Human Viral Pathogens from the Coronaviridae Family [0.03%]
冠状病毒家族的人类病原基因工程系统研究
S O Galkin,A N Anisenko,O A Shadrina et al.
S O Galkin et al.
The COVID-19 pandemic caused by the previously unknown SARS-CoV-2 Betacoronavirus made it extremely important to develop simple and safe cellular systems which allow manipulation of the viral genome and high-throughput screening of its pote...
Mutagenic Activity of AID/APOBEC Deaminases in Antiviral Defense and Carcinogenesis [0.03%]
AID/APOBEC 脱氨酶在抗病毒防御和致癌作用中的诱变活性
O N Shilova,D L Tsyba,E S Shilov
O N Shilova
Proteins of the AID/APOBEC family are capable of cytidine deamination in nucleic acids forming uracil. These enzymes are involved in mRNA editing, protection against viruses, the introduction of point mutations into DNA during somatic hyper...
In Silico Design of a New Multi-Epitope Peptide-Based Vaccine Candidate Against Q Fever [0.03%]
基于计算方法设计解热病多表位肽疫苗抗原新候选物
S Jabarzadeh,A Samiminemati,M Zeinoddini
S Jabarzadeh
Novel types of the vaccines with high immunogenicity and low risks, including epitope-based vaccines, are sought. Among zoonotic disease, Q fever caused by Coxiella burnetii is an important target due to numerous outbreaks and the pandemic ...
Immunogenic Properties of the DNA Construct Encoding the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein [0.03%]
用于编码SARS-CoV-2刺突蛋白受体结合域的DNA构建子的免疫原性属性研究
M B Borgoyakova,L I Karpenko,A P Rudometov et al.
M B Borgoyakova et al.
The development of preventive vaccines became the first order task in the COVID-19 pandemic caused by SARS-CoV-2. This paper reports the construction of the pVAX-RBD plasmid containing the Receptor-Binding Domain (RBD) of the S protein and ...
Development of Multiplex RT-PCR with Immobilized Primers for Identification of Infectious Human Pneumonia Pathogens [0.03%]
用于鉴定致病肺炎病原体的固定引物多重RT-PCR检测方法的研究开发进展
S A Lapa,R A Miftakhov,E S Klochikhina et al.
S A Lapa et al.
A prototype of a system for the detection of infectious human pneumonia pathogens based on multiplex solid-phase reverse transcription PCR (RT-PCR) was developed. Primers were designed to identify the DNA of six bacterial pneumonia pathogen...
Antiviral and Antimicrobial Nucleoside Derivatives: Structural Features and Mechanisms of Action [0.03%]
抗病毒和抗菌核苷衍生物的结构特征及其作用机制
A A Zenchenko,M S Drenichev,I A Il&#x;icheva et al.
A A Zenchenko et al.
The emergence of new viruses and resistant strains of pathogenic microorganisms has become a powerful stimulus in the search for new drugs. Nucleosides are a promising class of natural compounds, and more than a hundred drugs have already b...
A A Shulgin,T D Lebedev,V S Prassolov et al.
A A Shulgin et al.
The mechanisms involved in the origin and development of malignant and neurodegenerative diseases are an important area of modern biomedicine. A crucial task is to identify new molecular markers that are associated with rearrangements of in...
Preventive Vaccination with Mesenchymal Stem Cells Protects Mice from Lethal Infection Caused by Herpes Simplex Virus 1 [0.03%]
预防性接种间充质干细胞可保护小鼠免受单纯疱疹病毒1型致命感染的影响
R R Klimova,N A Demidova,O V Masalova et al.
R R Klimova et al.
Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) infect almost all organs and tissues, cause genital herpes-the most common sexually transmitted disease-disorders of the central nervous system (CNS), and lead to severe complications in chil...
Modification of the Spike Protein for Vaccines against Enveloped RNA Viruses [0.03%]
针对包膜RNA病毒疫苗的刺突蛋白改造
A N Vzorov,E I Samokhvalov,V V Chebanenko et al.
A N Vzorov et al.
Most vaccines work by inducing neutralizing antibodies that target the viral envelope. Enveloped RNA viruses have evolved mechanisms for surface glycoproteins to evade host immune responses, which exhibit substantial variability, even among...
A Luciferase-EGFP Reporter System for the Evaluation of DNA Methylation in Mammalian Cells [0.03%]
哺乳动物细胞中DNA甲基化的评价的荧光素酶-EGFP报告系统
X X Wang,H J Jia,Y R Lv et al.
X X Wang et al.
DNA methylation is an essential epigenetic modification involved in numerous biological processes. Here, we present a cell-based system pLTR-Luc2P-EGFP for evaluation of DNA methylation in mammalian cells. In this system, the expression of ...