登录
Distance-Dependent Fluorescence Quenching of p-Bis[2-(5-phenyloxazolyl)]benzene by Various Quenchers [0.03%] 邻苯二甲腈衍生物的浓度猝灭及动态猝灭系数测定
Bogumil Zelent,Józef Kuśba,Ignacy Gryczynski et al. Bogumil Zelent et al.
We report results of frequency-domain and steady-state measurements of the fluorescence quenching of p-bis-[2-(5-phenyloxazolyl)]benzene (POPOP) when quenched by bromoform (CHBr3), methyl iodide (CH3I), potassium iodide (KI), 1,2,4-trimetho...
Light Quenching and Fluorescence Depolarization of Rhodamine B and Applications of This Phenomenon to Biophysics [0.03%] 罗丹明B的光猝灭及荧光衰减和这类现象在生物物理中的应用
Joseph R Lakowicz,Ignacy Gryczyński,Valery Bogdanov et al. Joseph R Lakowicz et al.
The fluorescence intensity of rhodamine B (RhB) was found to display a sublinear dependence on incident power when excited with the focused output of a cavity-dumped dye laser. This effect was found to be proportional to the amplitude of th...
Transient Effects in Fluorescence Quenching Measured by 2-GHz Frequency-Domain Fluorometry [0.03%] 利用2GHz频域荧光仪测量荧光淬灭的瞬态效应
Joseph R Lakowicz,Michael L Johnson,Ignazy Gryczynski et al. Joseph R Lakowicz et al.
We used harmonic-content frequency-domain fluorometry to investigate the quenching of indole fluorescence by iodide and acrylamide in aqueous solution. The time-resolved intensity decays were recovered from the frequency response of the flu...
Light Quenching of Fluorescence Using Time-Delayed Laser Pulses As Observed by Frequency-Domain Fluorometry [0.03%] 利用频率域荧光法观察到的由时间延迟的激光脉冲引起的荧光猝灭现象
Ignacy Gryczynski,Józef Kuśba,Joseph R Lakowicz Ignacy Gryczynski
We describe experimental observations of fluorescence quenching by time-delayed light pulses whose wavelength overlaps the emission spectrum of 4-(dimethylamino)-4'-cyanostilbene (DCS). The relative cross sections for light quenching were p...
Influence of Diffusion on Excitation Energy Transfer in Solutions by Gigahertz Harmonic Content Frequency-Domain Fiuorometry [0.03%] 扩散对溶液中激发能量转移的吉赫兹谐波频率域荧光法研究影响
Joseph R Lakowicz,Henryk Szmacinski,Ignacy Gryczynski et al. Joseph R Lakowicz et al.
We report the effects of diffusion on fluorescence resonance energy transfer, as observed by using the frequency-domain technique. Energy transfer between indole (donor) and dansylamide (acceptor) was studied in low and high viscosity solut...
Time-Resolved Fluorescence Intensity and Anisotropy Decays of 2,5-Diphenyloxazole by Two-Photon Excitation and Frequency-Domain Fluorometry [0.03%] 双光子激发下2,5-二苯基恶唑的荧光强度及偏振衰减的时变特征研究
J R Lakowicz,I Gryczynski,Z Gryczynski et al. J R Lakowicz et al.
We report the first time-resolved fluorescence measurements of the intensity and anisotropy decays resulting from two-photon excitation. A 10-GHz frequency-domain fluorometer (Rev. Sci. Instrum 1990, 61, 2331), equipped with two focal lense...
Fluorescence Spectral Properties of 2,5-Diphenyl-1,3,4-oxadiazole with Two-Color Two-Photon Excitation [0.03%] 两种颜色双光子激发下茋二噁唑的荧光性质研究
Joseph R Lakowicz,Ignacy Gryczynski,Henryk Malak et al. Joseph R Lakowicz et al.
We observed fluorescence emission from 2,5-diphenyl-1,3,4-oxadiazole (PPD) resulting from two-photon excitation with two different wavelengths near 380 and 760 nm. For this two-color two-photon (2C2P) excitation the emission spectra and int...
Effect of Fluorescence Quenching by Stimulated Emission on the Spectral Properties of a Solvent-Sensitive Fluorophore [0.03%] 受激辐射引起的荧光淬灭对一种溶剂敏感染料的光谱性质的影响
Ignacy Gryczynski,Józef Kuśba,Zygmunt Gryczynski et al. Ignacy Gryczynski et al.
We examined the emission spectra and wavelength-dependent anisotropies of the solvent-sensitive fluorophore 4-(dimethylamino)-4′-cyanostilbene (DCS) under condition of fluorescence quenching by stimulated emission. The sample was illuminat...
End-to-End Diffusion and Distance Distributions of Flexible Donor-Acceptor Systems Observed by Intramolecular Energy Transfer and Frequency-Domain Fluorometry; Enhanced Resolution by Global Analysis of Externally Quenched and Nonquenched Samples [0.03%] 分子内能量转移和频域荧光法观测到的刚性供体-受体系统末端扩散和距离分布；通过全局分析外部淬灭和非淬灭样品提高分辨率
Joseph R Lakowicz,Józef Kuśba,Ignacy Gryczynski et al. Joseph R Lakowicz et al.
We used time-dependent fluorescence energy transfer of externally quenched and nonquenched samples, and global analysis of the data, to recover the end-to-end distance distributions and diffusion coefficients of flexible fluorescent molecul...
H LOTZKAR,T H SCHULTZ, et al. H LOTZKAR