Evaluation of both image resolution and contrast-to-noise ratio in scanning electron microscopy [0.03%]
扫描电子显微镜成像分辨率和对比度噪声比的评估
Tohru Ishitani,Mitsugu Sato
Tohru Ishitani
The contrast-to-gradient (CG) resolution has been defined as a weighted harmonic mean of the local resolution, i.e. the length defining the object's local fine structure at each pixel position. The newly defined resolution Res is defined as...
Kazuhiro Mio,Toshihiko Ogura,Shigeki Kiyonaka et al.
Kazuhiro Mio et al.
Single particle analysis is a computer-aided method for determining protein structure using particle images obtained by electron microscopy. This technique has great advantages, especially for analyzing fragile membrane-integrated proteins ...
Single particle conformations of human serum albumin by electron microscopy [0.03%]
透射电镜观察人血清白蛋白的单分子构象变异形式
Yutaka Ueno,Muneyo Mio,Chikara Sato et al.
Yutaka Ueno et al.
Using single particle images taken by electron microscopy, pH-dependent conformational changes of human serum albumin were investigated. Despite the noisy particle images of negatively stained serum albumin (67 kDa), our novel algorithm for...
A genetically encoded metallothionein tag enabling efficient protein detection by electron microscopy [0.03%]
一种遗传编码的金属硫蛋白标签使蛋白质能够通过电子显微镜有效检测
Yuri Nishino,Takuo Yasunaga,Atsuo Miyazawa
Yuri Nishino
The observation of biological materials by transmission electron microscopy (TEM) frequently requires the use of negative staining and/or immuno-gold labeling to visualize or identify the specimen, but these techniques are limited. In contr...
A statistically harmonized alignment-classification in image space enables accurate and robust alignment of noisy images in single particle analysis [0.03%]
统计上协调的图像空间对准分类能够使单颗粒分析中的噪声图像实现准确且稳健的对准
Masaaki Kawata,Chikara Sato
Masaaki Kawata
In determining the three-dimensional (3D) structure of macromolecular assemblies in single particle analysis, a large representative dataset of two-dimensional (2D) average images from huge number of raw images is a key for high resolution....
Sumie Katayama,Taiyun Wei,Toshihiro Omura et al.
Sumie Katayama et al.
When rice dwarf virus (RDV), a member of the Reoviridae family, infects leafhopper cells, formation of protruding tubules composed of nonstructural viral protein Pns 10 can be observed. We examined the three-dimensional (3D) structure of th...
Electron-beam-induced-current investigation of GaN/AlGaN/Si heterostructures using scanning transmission electron microscopy [0.03%]
扫描透射电子显微镜下电子束诱发电流在GaN/AlGaN/Si异质结构中的研究
Shigeyasu Tanaka,Kentaro Aoyama,Mikio Ichihashi et al.
Shigeyasu Tanaka et al.
An electron-beam-induced-current technique has been applied to scanning transmission electron microscopy to characterize GaN/AlGaN/n-Si heterostructures. The structure was formed by metalorganic vapor phase epitaxy using AlGaN as an interme...
Electron microscopic analysis of a fusion protein of postsynaptic density-95 and metallothionein in cultured hippocampal neurons [0.03%]
培养的海马神经元中 postsynaptic density-95 和金属硫蛋白融合蛋白的电子显微镜分析
Yuko Fukunaga,Ai Hirase,Hyeji Kim et al.
Yuko Fukunaga et al.
The subcellular localization of biomolecules at high resolution has traditionally been investigated by combining transmission electron microscopy (TEM) and chemical staining with heavy metals or immuno-based labeling with gold-conjugated an...
Structural changes of microvessels in the extensor digitorum longus muscle of the aged rat [0.03%]
老龄大鼠腓肠肌微血管结构的改变
Junzo Desaki,Naoya Nishida
Junzo Desaki
We examined the structural changes of microvessels (arterioles, capillaries, and venules) in the extensor digitorum longus muscle of the aged (27 months) rat. Muscle bundles in the aged muscle almost consisted of medium-sized muscle fibers ...