Cellular death linked to irreversible stress in the sarcoplasmic reticulum: the effect of inhibiting Ca(2+) -ATPase or protein glycosylation in the myocardiac cell model H9c2 [0.03%]
与肌浆网不可逆性压力相关的细胞死亡：心肌H9c2细胞模型中抑制Ca(2+)-ATP酶或蛋白糖基化的效应
Fernando Soler,Antonio Lax,Francisco Fernández-Belda
Fernando Soler
Experimental sarcoplasmic reticulum damage induced by 3 microM thapsigargin or 1 microg/ml tunicamycin provoked viability loss of the cell population in approximately 72 h. Release of cytochrome c from mitochondria was an early event and Ba...
Effects of different osmolytes on the induced folding of the N-terminal activation domain (AF1) of the glucocorticoid receptor [0.03%]
不同渗透调节物对糖皮质激素受体N端激活域（AF1）诱导折叠的影响
R Kumar,J M Serrette,S H Khan et al.
R Kumar et al.
In order to understand gene regulation by glucocorticoids, it is pivotal to know how the major transactivation domain AF1 of the glucocorticoid receptor (GR) functions. Located in the N-terminal region of the GR, AF1 is quantitatively impor...
Molecular basis of the interaction between IGFBP-3 and retinoid X receptor: role in modulation of RAR-signaling [0.03%]
IGFBP-3与核受体的相互作用及其对RAR信号转导调控的作用机制研究
Lynette J Schedlich,Lloyd D Graham,Michelle K O&#x;Han et al.
Lynette J Schedlich et al.
IGFBP-3 interacts with the retinoid X receptor-alpha (RXRalpha) and retinoic acid receptor-alpha (RARalpha) and thereby interferes with the formation of RXR:RAR heterodimers. Here we identify the domains in RXRalpha and IGFBP-3 that partici...
Depletion of S-adenosyl-l-methionine with cycloleucine potentiates cytochrome P450 2E1 toxicity in primary rat hepatocytes [0.03%]
丙氧苯所致S-腺苷-L-蛋氨酸耗竭加重细胞色素P450 2E1对大鼠原代肝细胞的毒性作用
Jian Zhuge,Arthur I Cederbaum
Jian Zhuge
S-Adenosyl-l-methionine (SAM) is the principal biological methyl donor. Methionine adenosyltransferase (MAT) catalyzes the only reaction that generates SAM. Hepatocytes were treated with cycloleucine, an inhibitor of MAT, to evaluate whethe...
Catechol-estrogen modified DNA: a better antigen for cancer autoantibody [0.03%]
儿茶酚雌激素修饰的DNA：一种更好的肿瘤自身抗体抗原
Wahid Ali Khan,Khursheed Alam,Moinuddin
Wahid Ali Khan
Estrogens are known mutagenic and carcinogenic risk factors. Non-enzymatic oxidation of catechol-estrogens in the presence of copper is reported to generate reactive oxygen species (ROS) that can cause DNA damage. We show that DNA modificat...
Regulation of protein kinase CK1alphaLS by dephosphorylation in response to hydrogen peroxide [0.03%]
蛋白激酶CK1α通过去磷酸化调节对过氧化氢的响应
Shahinaz Bedri,Stephanie M Cizek,Iryna Rastarhuyeva et al.
Shahinaz Bedri et al.
Low levels of hydrogen peroxide (H(2)O(2)) are mitogenic to mammalian cells and stimulate the hyperphosphorylation of heterogeneous nuclear ribonucleoprotein C (hnRNP-C) by protein kinase CK1alpha. However, the mechanisms by which CK1alpha ...
A pinoresinol-lariciresinol reductase homologue from the creosote bush (Larrea tridentata) catalyzes the efficient in vitro conversion of p-coumaryl/coniferyl alcohol esters into the allylphenols chavicol/eugenol, but not the propenylphenols p-anol/isoeugenol [0.03%]
刺果藤(Larrea tridentata)中的松脂醇还原酶同系物催化香豆醇/桂皮醇酯类化合物体外高效转化为丁香酚/肉桂酚，但不能将后者转化为对范烯丙基苯酚/异丁香烯酚
Daniel G Vassão,Sung-Jin Kim,Jessica K Milhollan et al.
Daniel G Vassão et al.
The creosote bush (Larrea tridentata) accumulates a complex mixture of 8-8' regiospecifically linked lignans, of which the potent antioxidant nordihydroguaiaretic acid (NDGA) is the most abundant. Its tetra-O-methyl derivative (M4N) is show...
Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations [0.03%]
人类痘病毒相关激酶1(VRK1)优势表位的鉴定及其不同胞内亚群的检测
Alberto Valbuena,Inmaculada López-Sánchez,Francisco M Vega et al.
Alberto Valbuena et al.
The human VRK1 is a new ser-thr kinase expressed in many cell types. VRK1 is a regulator of p53 and other transcription factors related with cellular responses to stress. The human VRK1 protein has a dominant epitope located in its C-termin...
Suppression of c-Src activity stimulates muscle differentiation via p38 MAPK activation [0.03%]
c-Src活性的抑制通过活化p38MAPK促进肌细胞分化
Min Jin Lim,Yong Hak Seo,Kyu Jin Choi et al.
Min Jin Lim et al.
Role of c-Src in muscle differentiation has been controversial. Here, we investigated if c-Src positively or negatively regulates muscle differentiation, using H9c2 and C2C12 cell lines. Inhibition of c-Src by treatment with PP1 and SU6656,...
Mechanistic studies on the intramolecular one-electron transfer between the two flavins in the human endothelial NOS reductase domain [0.03%]
关于人内皮型一氧化氮合酶还原结构域中两个黄素间的单电子 intramolecular 传递的机制研究
Yoshitaka Nishino,Keita Yamamoto,Shigenobu Kimura et al.
Yoshitaka Nishino et al.
The object of this study was to clarify the mechanism of electron transfer in the human endothelial nitric oxide synthase (eNOS) reductase domain using recombinant eNOS reductase domains; the FAD/NADPH domain containing FAD- and NADPH-bindi...