The Effect of Divalent Cations on the Thermostability of Type II Polyketide Synthase Acyl Carrier Proteins [0.03%]
二价阳离子对II型聚酮合酶乙酰载体蛋白热稳定性的效果研究
Marco Rivas,Valentine C Courouble,Miranda C Baker et al.
Marco Rivas et al.
The successful engineering of biosynthetic pathways hinges on understanding the factors that influence acyl carrier protein (ACP) stability and function. The stability and structure of ACPs can be influenced by the presence of divalent cati...
Multi-Model Sensor Fault Detection and Data Reconciliation: A Case Study with Glucose Concentration Sensors for Diabetes [0.03%]
多模型传感器故障检测与数据校正：以糖尿病血糖浓度传感器为例的研究
Jianyuan Feng,Iman Hajizadeh,Xia Yu et al.
Jianyuan Feng et al.
Erroneous information from sensors affect process monitoring and control. An algorithm with multiple model identification methods will improve the sensitivity and accuracy of sensor fault detection and data reconciliation (SFD&DR). A novel ...
Three-component solids velocity measurements in the outlet section of a riser [0.03%]
提升管出口段三相固气速度测量
Maria N Pantzali,Javier Marqués de Marino,Guy B Marin et al.
Maria N Pantzali et al.
Coincident (simultaneous) three-component particle velocity measurements performed using two laser Doppler anemometry probes at the outlet section of a 9 m high cylindrical riser are for the first time presented for dilute flow conditions. ...
Application of chemical reaction engineering principles to 'body-on-a-chip' systems [0.03%]
化学反应工程原理在人体芯片系统中的应用
Jong Hwan Sung,Ying I Wang,Jung Hun Kim et al.
Jong Hwan Sung et al.
The combination of cell culture models with microscale technology has fostered emergence of in vitro cell-based microphysiological models, also known as organ-on-a-chip systems. Body-on-a-chip systems, which are multi-organ systems on a chi...
Metastatic cancer cell attachment to endothelium is promoted by endothelial glycocalyx sialic acid degradation [0.03%]
内皮糖胺聚糖去唾液酸化促进癌细胞转移附着血管内皮细胞
Solomon A Mensah,Ian C Harding,Michelle Zhang et al.
Solomon A Mensah et al.
While it is known that cancer cell interactions with vascular endothelial cells (ECs) drive metastatic cancer cell extravasation from blood vessels into secondary tumor sites, the mechanisms of action are still poorly understood. Here, we t...
D2O Labeling to Measure Active Biosynthesis of Natural Products in Medicinal Plants [0.03%]
D2O标记用于测量药用植物中天然产物主动生物合成活性
Ryan S Nett,Xin Guan,Kevin Smith et al.
Ryan S Nett et al.
Plant natural products have served as a prominent source of medicines throughout human history, and are still used today as clinically-approved pharmaceuticals. However, many medicinal plants that produce useful compounds are slow-growing o...
Dielectric barrier discharge plasma microbubble reactor for pretreatment of lignocellulosic biomass [0.03%]
用于木质纤维素生物质预处理的介质阻挡放电等离子体微气泡反应器
Alexander Wright,Hemaka Bandulasena,Christopher Ibenegbu et al.
Alexander Wright et al.
A novel lignocellulosic biomass pretreatment reactor has been designed and tested to investigate pretreatment efficacy of miscanthus grass. The reactor was designed to optimize the transfer of highly oxidative species produced by dielectric...
Probing the Acyl Carrier Protein-Enzyme Interactions within Terminal Alkyne Biosynthetic Machinery [0.03%]
探针酰基载体蛋白-酶相互作用的末端炔烃生物合成机器
Michael Su,Xuejun Zhu,Wenjun Zhang
Michael Su
The alkyne functionality has attracted much interest due to its diverse chemical and biological applications. We recently elucidated an acyl carrier protein (ACP)-dependent alkyne biosynthetic pathway, however, little is known about ACP int...
Bioinformatic analysis of Chinese hamster ovary host cell protein lipases [0.03%]
CHO宿主细胞蛋白脂肪酶的生物信息学分析
Madolyn L MacDonald,Nathaniel Hamaker,Kelvin H Lee
Madolyn L MacDonald
Complete, accurate genome assemblies are necessary to design targets for genetic engineering strategies. Successful gene knockdowns and knockouts in Chinese hamster ovary (CHO) cells may prevent the expression of difficult-to-remove host ce...
Coupling Brain Perfusion Screens and Next Generation Sequencing to Identify Blood-Brain Barrier Binding Antibodies [0.03%]
通过脑灌注扫描和二代测序技术鉴定血脑屏障结合抗体中的耦合筛查
C Christopher Stutz,Julia V Georgieva,Eric V Shusta
C Christopher Stutz
Antibodies that target the blood-brain barrier (BBB) in vivo are of particular interest for the treatment of neurological diseases. Here, we screened a phage display single-chain antibody (scFv) library by brain perfusion in an attempt to i...