Compartmentalization of pathway sequential enzymes into synthetic protein compartments for metabolic flux optimization in Escherichia coli [0.03%]
工程化蛋白质隔室用于优化大肠杆菌途径顺序酶的代谢通量
Li Wan,Yingying Zhu,Juntao Ke et al.
Li Wan et al.
Advancing the formation of artificial membraneless compartments with organizational complexity and diverse functionality remains a challenge. Typically, synthetic compartments or membraneless organelles are made up of intrinsically disorder...
Xianhao Xu,Xueqin Lv,Xinyu Bi et al.
Xianhao Xu et al.
In recent years, genetic circuit-based regulation of metabolic flux in microbial cell factories has received significant attention. In this review, we describe a pipeline for the design and construction of genetic circuits for metabolic flu...
Metabolic flux optimization of iterative pathways through orthogonal gene expression control: Application to the β-oxidation reversal [0.03%]
通过正交基因表达控制优化迭代途径的代谢通量：应用于β-氧化逆转
Seung Hwan Lee,Yang Hu,Alexander Chou et al.
Seung Hwan Lee et al.
Balancing relative expression of pathway genes to minimize flux bottlenecks and metabolic burden is one of the key challenges in metabolic engineering. This is especially relevant for iterative pathways, such as reverse β-oxidation (rBOX) ...
Riboswitch-guided chalcone synthase engineering and metabolic flux optimization for enhanced production of flavonoids [0.03%]
核糖开关引导的查尔酮合成酶工程和代谢通量优化以增强黄酮类化合物的生产
Hyun Gyu Hwang,Alfonsina Milito,Jae-Seong Yang et al.
Hyun Gyu Hwang et al.
Flavonoids are a group of secondary metabolites from plants that have received attention as high value-added pharmacological substances. Recently, a robust and efficient bioprocess using recombinant microbes has emerged as a promising appro...
Systematic Analysis of Bottlenecks in a Multibranched and Multilevel Regulated Pathway: The Molecular Fundamentals of l-Methionine Biosynthesis in Escherichia coli [0.03%]
大肠杆菌L-蛋氨酸生物合成途径的多层次多分支调控系统的瓶颈分析：分子基础
Jian-Feng Huang,Zhen-Yang Shen,Qiao-Li Mao et al.
Jian-Feng Huang et al.
However, in most successful cases, the target chemicals are synthesized through a linear pathway, and regular methodologies for the identification of bottlenecks and metabolic flux optimization in multibranched and multilevel regulated pathways, such as the l-methionine biosynthetic pathway, have rarely
One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli [0.03%]
通过色氨酸衰减子失活和启动子置换一步法提高大肠杆菌L-色氨酸产量
Pengfei Gu,Fan Yang,Junhua Kang et al.
Pengfei Gu et al.
In order to construct a microbial strain for efficient L-tryptophan production from glucose, we developed a one step tryptophan attenuator inactivation and promoter swapping strategy for metabolic flux optimization after a base strain was obtained by overexpressing the tktA, mutated trpE and aroG genes
Chikara Furusawa,Kunihiko Kaneko
Chikara Furusawa
The present scheme may help explain a wide range of cellular adaptive responses including the metabolic flux optimization for maximal cell growth.