In recent years, genetic circuit-based regulation of metabolic flux in microbial cell factories has received significant attention. In this review, we describe a pipeline for the design and construction of genetic circuits for metabolic flux optimization. In particular, we summarize the recent advances in computationally assisted prediction of critical metabolic nodes and genetic circuit design automation. Further, we introduce strategies for constructing high-performance genetic circuits. We also summarize the latest applications of genetic circuits in the dynamic regulation of metabolism and high-throughput screening. Finally, we discuss the challenges and prospects associated with the design and construction of sophisticated genetic circuits. Through this review, we aim to provide a theoretical basis for designing and constructing high-performance genetic circuits to optimize metabolic flux.

Keywords: dynamic regulation; genetic circuit; high-throughput screening; synthetic biology.

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近年来，基于基因电路调节微生物细胞工厂中的代谢流受到了广泛关注。在这篇综述中，我们描述了一种用于设计和构建优化代谢流的基因电路的流程。特别地，我们总结了计算机辅助预测关键代谢节点和基因电路设计自动化方面的近期进展。此外，我们介绍了构建高性能基因电路的战略。我们还总结了基因电路在动态调节代谢和高通量筛选中的最新应用。最后，我们讨论了设计和构建复杂基因电路所面临的挑战和前景。通过这篇综述，我们的目标是为设计和构建优化代谢流的高性能基因电路提供理论基础。

关键词：动态调控；基因电路；高通量筛选；合成生物学。

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