Profiling Cytosolic Drug Delivery in Mammalian Cells: A Generalizable Assay for Intracellular Accumulation [0.03%]
哺乳动物细胞胞质药物递送的剖析：一种细胞内蓄积的普适性测定方法
Sobika Bhandari,George M Ongwae,Rachita Dash et al.
Sobika Bhandari et al.
The CHAMP assay utilizes HaloTag-expressing cells and strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry to quantify the presence of azide-tagged test compounds in the cytosol.
Sequential Radiosynthetic Methodology: Assembly of Boronic Ester Precursors on a Diol Resin and Radioiodination Using Copper-Mediated Radioiododeboronation and Azide-Alkyne Cycloaddition [0.03%]
序贯放射合成法：在二醇树脂上装配硼酸酯前体，并通过铜介导的放射性碘脱硼化和叠氮-炔环加成进行放射性碘标记
Yuto Kondo,Hiroyuki Kimura,Mamiko Tanaka et al.
Yuto Kondo et al.
A streamlined radiosynthetic methodology enables the precise sequential assembly of boronic ester precursors on diol resin, seamlessly integrating copper-mediated radioiododeboronation and azide-alkyne cycloaddition. This approach yields ra...
4'- C-Cholesterol/Pyridyl-2'- O-Methyl Uridine-Functionalized siRNA Enhances Stability and Carrier-Free Gene Silencing [0.03%]
4'-C-胆固醇/尿嘧啶-2' -O-甲基功能化siRNA增强稳定性和无载体基因沉默
Santanu Sar,Shalini Gupta,Gourav Das et al.
Santanu Sar et al.
In this study, we reported the synthesis of 4'-C-cholesterol-2'-O-methyl (4'-C-chol-2'-OMe) and 4'-C-methylpyridine-2'-O-methyl (4'-C-Mpy-2'-OMe) uridine conjugates via copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and their incorporation at the 3'-overhangs of the siRNA duplex.
Design and Synthesis of Thymol Derivatives Bearing a 1,2,3-Triazole Moiety for Papaya Protection against Fusarium solani [0.03%]
设计并合成一类新型噻霉酮三氮唑衍生物用于防治番木瓜腐皮镰孢菌病
Mariana Belizário de Oliveira,Poliana Aparecida Rodrigues Gazolla,Leandra Martins Meireles et al.
Mariana Belizário de Oliveira et al.
In this study, a series of 20 novel thymol derivatives, incorporating a 1,2,3-triazole moiety, were synthesized via a three-step process, with the key step being the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction.
Ene-azide chemistry in the synthesis of 1,2,3-triazoline/triazole and the corresponding mechanistic aspects [0.03%]
叠氮化学在三氮唑/三氮唑啉合成中的应用及相应机理研究
Kunj B Mishra
Kunj B Mishra
The ene-azide cycloaddition reaction appears as an alternative to azide-alkyne cycloaddition for the synthesis of 1,2,3-triazole building blocks.
Exploring Multivalent Architectures for Binding and Stabilization of N-Acetylgalactosamine 6-Sulfatase [0.03%]
用于结合和稳定N-乙酰半乳糖胺-6-硫酸酯酶的多价结构研究
Maria Giulia Davighi,Francesca Clemente,Giampiero D&#x;Adamio et al.
Maria Giulia Davighi et al.
We report in this work the synthesis of a library of multivalent glycomimetics exploiting the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction between several dendrimeric scaffolds armed with terminal alkynes and azido ending iminosugars of different structures (pyrrolidines, piperidines
Tuning Hyaluronic Acid Microstructures by Engineered Amphiphilicity: From Dynamically Cross-Linked Gels to Multilayered Nanoparticles [0.03%]
通过工程两亲性调节透明质酸微结构：从动态交联凝胶到多层纳米粒子
Shing-Yun Chang,Aidan P McAnena,Joshua Kim et al.
Shing-Yun Chang et al.
We show that the amphiphilicity and microstructures of hydrophilic hyaluronic acid (HA) can be explicitly tuned by the stoichiometric integration of cholesterol to azide-functionalized HA via strain-promoted azide-alkyne cycloaddition (SPAAC).
inCu-click: DNA-enhanced ligand enables live-cell, intracellular click chemistry reaction with copper catalyst [0.03%]
活细胞内含铜催化剂的点击化学反应：一种增强型配体诱导铜分子活性参与反应(inCu-click：DNA增强配体使铜催化活细胞内的点击化学反应)
Keqing Nian,Yifang Liu,Yuchen Qiu et al.
Keqing Nian et al.
Labeling cellular biomolecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC) offers rapid reaction kinetics and uses small azide and alkyne probes that minimally disturb molecular function, making it ideal for tracking biomolecules.
Identification and characterization of O-GlcNAc modifications of a conserved orthopoxvirus core protein [0.03%]
正痘病毒核心蛋白O-GlcNAc修饰的鉴定与表征
Yunliang Zhang,Bernard Moss
Yunliang Zhang
A virion protein of ~40 kDa was identified by SDS-polyacrylamide gel electrophoresis following azide-alkyne cycloaddition of biotin or an infrared dye to O-GlcNAc residues.
Solid-phase DNA-encoded library synthesis: a master builder's instructions [0.03%]
固相DNA编码化合物库合成：编者手册
Anjali Dixit,Brian M Paegel
Anjali Dixit
The protocol begins with on-bead synthesis of a linker containing a spectroscopic handle for chromatographic analysis, an ionization enhancer for mass spectrometry and an alkyne for installation of DNA encoding sites via copper-catalyzed azide-alkyne cycloaddition click chemistry.
耗时 0.11926 秒，为您在
48223910
条记录里面共找到 1806 篇文章 [XML]