Identification of Cytosol-Facing Organelle Membrane-Proximal Proteins via Proximity-Dependent Biotinylation [0.03%]
基于生物素标记的细胞器膜近端蛋白的鉴定方法
Xinyue Bao,Xiaoyan Zhang,Huifang Jia et al.
Xinyue Bao et al.
Organelle membrane (OM)-resident or -proximal proteins that face the cytosol play a critical role in the import and exchange of ions, metabolites, and proteins between subcellular compartments, thereby regulating organelle biogenesis and fu...
Key challenges and recommendations for defining organelle membrane contact sites [0.03%]
定义细胞器膜接触位点的关键挑战及建议
Tito Calì,Emmanuelle M Bayer,Emily R Eden et al.
Tito Calì et al.
Intracellular membrane contact sites (MCSs) between organelles have crucial roles in cellular signalling and homeostasis. These sites, which are often disrupted in pathological conditions, enable the exchange of ions, lipids and metabolites...
Yoshihiko Kuchitsu,Tomohiko Taguchi
Yoshihiko Kuchitsu
Our body is constantly exposed to pathogens, and equipped with a highly elaborate immune system to fight against invading pathogens. The first line of defense is the innate immune system. It has evolved to detect conserved microbial molecul...
Yuto Nagashima,Tomoya Eguchi,Ikuko Koyama-Honda et al.
Yuto Nagashima et al.
Disintegration of organelle membranes induces various cellular responses and has pathological consequences, including autoinflammatory diseases and neurodegeneration. Establishing methods to induce membrane rupture of specific organelles is...
Organelle-Targeted Laurdans Measure Heterogeneity in Subcellular Membranes and Their Responses to Saturated Lipid Stress [0.03%]
亚细胞膜定位的罗丹明探针测量异质性和饱和脂质胁迫响应
Adrian M Wong,Itay Budin
Adrian M Wong
Organelles feature characteristic lipid compositions that lead to differences in membrane properties. In cells, membrane ordering and fluidity are commonly measured using the solvatochromic dye Laurdan, whose fluorescence is sensitive to li...
Optical recordings of organellar membrane potentials and the components of membrane conductance in lysosomes [0.03%]
线粒体和溶酶体膜电位及溶酶体膜通透性的光学记录
Cristian Castillo-Velasquez,Ella Matamala,Diego Becerra et al.
Cristian Castillo-Velasquez et al.
The eukaryotic cell is highly compartmentalized with organelles. Owing to their function in transporting metabolites, metabolic intermediates and byproducts of metabolic activity, organelles are important players in the orchestration of cel...
Membraneless and membrane-bound organelles in an anhydrobiotic cell line are protected from desiccation-induced damage [0.03%]
无膜和有膜细胞器在耐干燥性细胞系中免受脱水诱导的损伤
Clinton J Belott,Oleg A Gusev,Takahiro Kikawada et al.
Clinton J Belott et al.
Anhydrobiotic species can survive virtually complete water loss by entering a reversible ametabolic glassy state that may persist for years in ambient conditions. The Pv11 cell line was derived from the egg mass of the anhydrobiotic midge, ...
How to identify close friends: mapping organelle membrane proximity proteomes [0.03%]
如何识别知己：定位细胞器膜邻近蛋白质组
Gwendolyn K Kirschner
Gwendolyn K Kirschner
The activation mechanism of Atg15, a vacuolar phospholipase required for the disintegration of organelle membranes [0.03%]
Atg15的活化机制：一种用于细胞器膜崩解的液泡磷脂酶
Yasunori Watanabe,Kuninori Suzuki
Yasunori Watanabe
The disintegration of cytoplasm-to-vacuole targeting (Cvt) bodies and autophagic bodies in vacuoles is essential to the Cvt pathway and macroautophagy in yeast. Atg15 is a vacuolar lipase required for the degradation of both Cvt and autopha...
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