The Journal of antibiotics. 2025 Jun 12. doi: 10.1038/s41429-025-00840-9 Q22.72025

Rare distribution of butenolide-type signaling molecules among Streptomyces strains and functional importance as inducing factors for secondary metabolite production in Streptomyces rochei 7434AN4

土霉素产生菌链霉菌ROCHEI 7434AN4中布泰内酸型信号分子的分布及诱导活性研究翻译改进

Asahi Hirata^{1 2}, Miho Sumiyoshi³, Hazuki Fujita^{2 4}, Momoko Akimoto^{1 2}, Mary Hannah Rose A Padayao^{1 2}, Yuto Eguchi¹, Maki Matsuura⁵, Miyuki Otsuka⁵, Kuninobu Inada⁶, Aiko Teshima^{1 2}, Kenji Arakawa^{7 8 9 10}

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作者单位

¹ Program of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan.

² Hiroshima Research Center for Healthy Aging (HiHA), Hiroshima University, Hiroshima, Japan.

³ Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan.

⁴ Faculty of Engineering, Hiroshima University, Hiroshima, Japan.

⁵ College of Agriculture, Tamagawa University, Machida, Tokyo, Japan.

⁶ Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan.

⁷ Program of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan. karakawa@hiroshima-u.ac.jp.

⁸ Hiroshima Research Center for Healthy Aging (HiHA), Hiroshima University, Hiroshima, Japan. karakawa@hiroshima-u.ac.jp.

⁹ Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan. karakawa@hiroshima-u.ac.jp.

¹⁰ Faculty of Engineering, Hiroshima University, Hiroshima, Japan. karakawa@hiroshima-u.ac.jp.

DOI: 10.1038/s41429-025-00840-9 PMID: 40506574

摘要中英对照阅读

Streptomyces rochei 7434AN4 produces two structurally unrelated polyketide antibiotics, lankacidin (LC) and lankamycin (LM), and their biosynthesis is tightly controlled by 2,3-disubstituted butenolide-type signaling molecules SRB1 and SRB2. We here investigated the distribution of 2,3-disubstituted butenolides (SRB-type butenolides) among randomly selected 122 Streptomyces strains using two approaches; (1) feeding of their culture extracts into an srrX-de... ...点击完成人机验证后继续浏览

链霉菌7434AN4（Streptomyces rochei）产生两种结构上不相关的聚酮类抗生素，即兰卡丁酸（LC）和兰卡霉素（LM），它们的生物合成受到2,3-位取代的丁烯内酯型信号分子SRB1和SRB2的严格调控。我们在此通过两种方法研究了随机选择的122种链霉菌株中2,3-位取代的丁烯内酯（SRB型丁烯内酯）的分布情况：（1）将它们的文化提取物喂入S. rochei缺损srrX基因的菌株KA20；（2）与KA20菌株共发酵。除链霉菌纤维素产生者（一种LC和LM生产者）外，所有随机选择的供体菌株均未能在KA20菌株中恢复LC和LM的产生。这些发现强烈表明SRB型丁烯内酯分子在链霉菌种中的罕见分布情况。一种SRB型丁烯内酯，即SAB1（它是诱导产尼可霉素的Streptomyces ansochromogenes的诱导物），即使浓度高达1 mM也无法在KA20菌株中恢复抗生素产生。此外，我们注意到当向KA20菌株喂入SRB1时，4-去羟基-SRB1作为一种新型化合物积累起来。纯化的4-去羟基-SRB1在50 µM（相对于天然SRB1的最低诱导浓度40 nM高出1000倍）下对KA20菌株没有抗生素产生的诱导活性。这些发现表明，在S. rochei中，C-2位连接的碳氢链长度和C-4位羟基对于诱导活性的重要性。

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期刊名：Journal of antibiotics

缩写：J ANTIBIOT

ISSN：0021-8820

e-ISSN：1881-1469

IF/分区：2.7/Q2

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Rare distribution of butenolide-type signaling molecules among Streptomyces strains and functional importance as inducing factors for secondary metabolite production in Streptomyces rochei 7434AN4

土霉素产生菌链霉菌ROCHEI 7434AN4中布泰内酸型信号分子的分布及诱导活性研究 翻译改进

土霉素产生菌链霉菌ROCHEI 7434AN4中布泰内酸型信号分子的分布及诱导活性研究翻译改进